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Gast (NM_010257) Mouse Tagged ORF Clone
CAT＃: MR200311
- TrueORF®
Gast (Myc-DDK-tagged) - Mouse gastrin (Gast)
ORF Plasmid: tGFP
"NM_010257" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | G; GAS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR200311 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGACTGTGTGTGTACATGCTGGTCTTAGTGCTGGCTCTAGCTACCTTCTCGGAAGCTTCTTGGA AGCCCCGCTCCCAGCTACAGGATGCATCATCTGGACCAGGGACCAATGAGGACCTGGAACAGCGCCAGTT CAACAAGCTGGGCTCAGCCTCTCACCATCGAAGGCAGCTGGGGCTCCAGGGTCCTCAACACTTCATAGCA GACCTGTCCAAGAAGCAGAGGCCACGAATGGAGGAAGAAGAAGAGGCCTACGGATGGATGGACTTTGGCC GCCGCAGTGCTGAGGAAGACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR200311 protein sequence
Red=Cloning site Green=Tags(s) MPRLCVYMLVLVLALATFSEASWKPRSQLQDASSGPGTNEDLEQRQFNKLGSASHHRRQLGLQGPQHFIA DLSKKQRPRMEEEEEAYGWMDFGRRSAEEDQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010257 |
| ORF Size | 306 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010257.1, NM_010257.2, NM_010257.3, NM_010257.4, NP_034387.2 |
| RefSeq Size | 455 bp |
| RefSeq ORF | 306 bp |
| Locus ID | 14459 |
| UniProt ID | P48757 |
| MW | 11.6 kDa |
| Gene Summary | This gene encodes the peptide hormone gastrin, which stimulates gastric acid secretion, proliferation, cell migration and angiogenesis, as well as inhibits apoptosis. The encoded preproprotein undergoes proteolytic processing to generate multiple gastrin peptides differing in size. Mice lacking the encoded protein exhibit a decrease in the number of parietal cells, achlorohydria and a decrease in the colonic proliferation. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204768 | Gast (untagged) - Mouse gastrin (Gast), (10ug) |
CNY 1,200.00 |
|
| MG200311 | Gast (tGFP-tagged) - Mouse gastrin (Gast) |
CNY 2,850.00 |
|
| MR200311L3 | Lenti ORF clone of Gast (Myc-DDK-tagged) - Mouse gastrin (Gast) |
CNY 4,750.00 |
|
| MR200311L4 | Lenti ORF clone of Gast (mGFP-tagged) - Mouse gastrin (Gast) |
CNY 4,750.00 |
