Mstn (NM_010834) Mouse Tagged ORF Clone
CAT#: MG227629
- TrueORF®
Mstn (tGFP-tagged) - Mouse myostatin (Mstn), (10ug)
ORF Plasmid: DDK
"NM_010834" in other vectors (4)
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CNY 5,256.00
Cited in 2 publications. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Cmpt; Gdf8 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227629 representing NM_010834
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCAAAAACTGCAAATGTATGTTTATATTTACCTGTTCATGCTGATTGCTGCTGGCCCAGTGGATC TAAATGAGGGCAGTGAGAGAGAAGAAAATGTGGAAAAAGAGGGGCTGTGTAATGCATGTGCGTGGAGACA AAACACGAGGTACTCCAGAATAGAAGCCATAAAAATTCAAATCCTCAGTAAGCTGCGCCTGGAAACAGCT CCTAACATCAGCAAAGATGCTATAAGACAACTTCTGCCAAGAGCGCCTCCACTCCGGGAACTGATCGATC AGTACGACGTCCAGAGGGATGACAGCAGTGATGGCTCTTTGGAAGATGACGATTATCACGCTACCACGGA AACAATCATTACCATGCCTACAGAGTCTGACTTTCTAATGCAAGCGGATGGCAAGCCCAAATGTTGCTTT TTTAAATTTAGCTCTAAAATACAGTACAACAAAGTAGTAAAAGCCCAACTGTGGATATATCTCAGACCCG TCAAGACTCCTACAACAGTGTTTGTGCAAATCCTGAGACTCATCAAACCCATGAAAGACGGTACAAGGTA TACTGGAATCCGATCTCTGAAACTTGACATGAGCCCAGGCACTGGTATTTGGCAGAGTATTGATGTGAAG ACAGTGTTGCAAAATTGGCTCAAACAGCCTGAATCCAACTTAGGCATTGAAATCAAAGCTTTGGATGAGA ATGGCCATGATCTTGCTGTAACCTTCCCAGGACCAGGAGAAGATGGGCTGAATCCCTTTTTAGAAGTCAA GGTGACAGACACACCCAAGAGGTCCCGGAGAGACTTTGGGCTTGACTGCGATGAGCACTCCACGGAATCC CGGTGCTGCCGCTACCCCCTCACGGTCGATTTTGAAGCCTTTGGATGGGACTGGATTATCGCACCCAAAA GATATAAGGCCAATTACTGCTCAGGAGAGTGTGAATTTGTGTTTTTACAAAAATATCCGCATACTCATCT TGTGCACCAAGCAAACCCCAGAGGCTCAGCAGGCCCTTGCTGCACTCCGACAAAAATGTCTCCCATTAAT ATGCTATATTTTAATGGCAAAGAACAAATAATATATGGGAAAATTCCAGCCATGGTAGTAGACCGCTGTG GGTGCTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227629 representing NM_010834
Red=Cloning site Green=Tags(s) MMQKLQMYVYIYLFMLIAAGPVDLNEGSEREENVEKEGLCNACAWRQNTRYSRIEAIKIQILSKLRLETA PNISKDAIRQLLPRAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQADGKPKCCF FKFSSKIQYNKVVKAQLWIYLRPVKTPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMSPGTGIWQSIDVK TVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTES RCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPIN MLYFNGKEQIIYGKIPAMVVDRCGCS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010834 |
ORF Size | 1128 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010834.3 |
RefSeq Size | 2682 bp |
RefSeq ORF | 1131 bp |
Locus ID | 17700 |
UniProt ID | O08689 |
Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Homozygous knockout mice for this gene exhibit increased muscle mass and bone density, and reduced adiposity. [provided by RefSeq, Jul 2016] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Overexpression of Latent TGFβ Binding Protein 4 in Muscle Ameliorates Muscular Dystrophy through Myostatin and TGFβ
,null,
PLoS Genetics
,PubMed ID 27148972
[Mstn]
|
Retraction: Pid1 Induces Insulin Resistance in Both Human and Mouse Skeletal Muscle during Obesity
,null,
Molecular Endocrinology
,PubMed ID 23927930
[Mstn]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208981 | Mstn (untagged) - Mouse myostatin (Mstn), (10ug) |
CNY 3,656.00 |
|
MR227629 | Mstn (Myc-DDK-tagged) - Mouse myostatin (Mstn) |
CNY 3,656.00 |
|
MR227629L3 | Lenti ORF clone of Mstn (Myc-DDK-tagged) - Mouse myostatin (Mstn) |
CNY 6,056.00 |
|
MR227629L4 | Lenti ORF clone of Mstn (mGFP-tagged) - Mouse myostatin (Mstn) |
CNY 6,056.00 |