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Prx (NM_198048) Mouse Tagged ORF Clone
CAT＃: MG227574
- TrueORF®
Prx (tGFP-tagged) - Mouse periaxin (Prx) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_198048" in other vectors (2)
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CNY 16,824.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | L-Periaxin |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227574 representing NM_198048
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCAGGAGCCGCAGCGCTGAGGAGCTGAGACGGGCGGAGTTGGTGGAGATTATCGTGGAGACCG AGGCACAGACCGGGGTCAGCGGCTTCAACGTAGCAGGCGGCGGCAAAGAAGGAATCTTTGTCCGTGAGCT GCGAGAGGACTCACCGGCAGCTAAGAGCCTCAGCTTGCAAGAAGGGGACCAGCTGCTGAGTGCCCGTGTG TTCTTTGAGAACTTCAAATATGAGGATGCACTTCGCCTGCTGCAATGCGCAGAGCCCTACAAGGTCTCCT TCTGCTTGAAGCGCACTGTGCCCACCGGGGATCTGGCACTGAGGCCCGGGACGGTGTCTGGATACGAGAT GAAGGGCCCACGGGCCAAAGTGGCCAAGCTGAACATCCAGAGTCTGGCCCCTGTGAAGAAGAAGAAGATG GTGACTGGGGCCCTGGGGACCCCTGCAGATTTGGCCCCTGTTGACGTCGAGTTCTCTTTTCCCAAGTTCT CCCGACTGCGTCGGGGTCTCAAAGCCGAGGCTGTCAAGGGACCTGTCCCAGCTGCCCCAGCCCGTCGCCG CCTCCAGCTGCCTCGGCTGCGTGTCCGAGAAGTAGCTGAAGAGGCCCAGGTAGCCCGAATGGCTGCTGCT GCTCCTCCCCCAAGGAAGGCCAAGGCAGAAGCTGAGGCAGCCACAGGAGCTGGGTTCACAGCCCCTCAGT TAGAGCTAGTTGGGCCTCGGCTGCCTAGTGCCGAGGTGGGTGTCCCTCAGGTCTCAGTTCCCAAGGGGAC CCCATCAACAGAGGCAGCCAGCGGCTTTGCCCTTCACCTGCCAACCCTTGGGCTAGGTGCCCCAGCTGCA CCGGCTGTGGAGCCCCCAGCCACGGGAATCCAGGTTCCACAAGTGGAACTCCCCACCCTGCCCTCTCTAC CCACTCTTCCCACACTTCCATGCCTGGACACCCAGGAAGGAGCTGCAGTGGTAAAAGTCCCTACCCTGGA TGTGGCAGCTCCGTCTATGGGGGTGGACCTGGCTTTGCCGGGTGCAGAGGTGGAGGCCCAGGGAGAGGTT CCTGAAGTGGCCCTCAAGATGCCCCGGCTCAGTTTCCCCCGTTTTGGGATTCGGGGGAAGGAAGCCACTG AAGCCAAAGTAGTCAAGGGCAGCCCTGAGGCCAAAGCAAAGGGTCCCAGACTTCGAATGCCCACCTTTGG GCTTTCTCTCCTGGAACCCCGGCCCTCTGGCCCTGAAGCTGTTGCTGAGAGCAAGCTGAAGCTACCCACC CTCAAGATGCCCTCTTTCGGCATTGGTGTGACTGGGCCTGAGGTCAAGGCACCCAAGGGGCCCGAAGTAA AGCTCCCTAAGGTTCCTGAGGTCAAACTCCCGAAAGTGCCCGAGGCAGCCATTCCAGATGTGCAACTCCC TGAGGTACAGCTGCCCAAAATGTCAGACATGAAACTTCCAAAGATCCCTGAGATGGTTGTACCCGACGTT CGTCTTCCGGAAGTGCAGCTGCCCAAAGTCCCTGAGATGAAAGTCCCAGAGATGAAGCTCCCGAAGGTGC CCGAGATGGCCGTGCCCGATGTACACCTTCCAGATGTACAGCTCCCGAAAGTCCCAGAGATGAAGCTCCC GAAGGTGCCCGAGATGGCCGTGCCCGATGTACACCTTCCAGATGTACAGCTCCCGAAAGTTCCAGAGATG AAGCTACCAGAGATGAAGCTCCCGAAGGTGCCGGAGATGGCCGTGCCGGATGTACGACTCCCGGAAGTTC AGCTGCCCAAAGTGTCTGAGGTGAAGCTCCCAAAGATGCCTGAGATGGCCGTGCCTGATGTCCACCTCCC GGAGCTACAACTTCCCAAAATGTCCGAGGTGAAGCTCCCAAAGATGCCCGAGATGGCCGTGCCCGATGTT CGCCTCCCGGAAGTTCAGCTGCCCAAAGTGTCAGAGATGAAACTCCCTAAGATGCCAGAGATGACCATGC CCGACATTCGCCTCCCAGAAGTTCAGTTGCCCAAAGTGCCTGACATTAAACTTCCTGAAATGAAGCTTCC AGAAATAAAACTCCCCAAAGTGCCTGACATGGCAGTGCCTGATGTCCCCCTTCCAGAGCTGCAGCTGCCC AAAGTGTCGGACATTCGGCTGCCTGAAATGCAAGTGTCACAGGTCCCAGAGGTGCAGCTTCCCAAGATGC CAGAGATGAAGTTGTCCAAGGTTCCTGAGGTACAGAGGAAATCTGCAGGGGCGGAGCAGGCAAAAGGGAC TGAATTTAGTTTCAAGTTGCCCAAGATGACCATGCCCAAGTTGGGGAAAGTGGGCAAGCCTGGGGAGGCA AGTATTGAGGTTCCAGACAAACTCATGACACTTCCCTGTCTGCAGCCAGAGGTGGGCACTGAGGCATCCC ATGTTGGTGTCCCTTCCCTCTCTCTCCCCTCTGTGGAGCTTGACTTGCCTGGGGCCCTGGGCCTGGAGGG ACAAGTCCAAGAAGCTGTCCCAGGCAAAGTGGAGAAGCCAGAGGGCCCCAGGGTAGCAGTGGGTGTTGGA GAGGTGGGCTTTCGTGTGCCCTCTGTGGAGATTGTCACTCCTCAGCTGCCCACAGTTGAAGTTGAGAAAG AGCAGCTAGAGATGGTGGAGATGAAAGTCAAACCCTCTTCCAAGTTCTCTCTGCCCAAATTCGGACTTTC AGGGCCCAAAGCTGTCAAGGGAGAGGTGGAGGGGCCTGGGCGAGCCACCAAGCTGAAGGTTTCCAAGTTT ACCATCTCACTTCCCAAAGCTCGAGCAGGGACTGAGGCCGAAGCGAAGGGAGCTGGGGAAGCCGGGTTGC TGCCAGCGCTGGATCTGTCCATCCCACAGCTCAGCCTGGATGCCCAGCTGCCCTCAGGCAAGGTGGAAGT AGCTGATAGCAAGCCTAAATCGTCCAGATTTGCTCTGCCCAAGTTTGGGGTGAAAGGCCGGGACTCTGAG GCTGATGTACTGGTGGCAGGGGAGGCTGAGCTTGAGGGAAAGGGTTGGGGCTGGGATGGGAAGGTGAAGA TGCCCAAGCTGAAAATGCCATCTTTTGGGTTGTCCCGAGGAAAGGAAGCAGAAACTCAGGATGGACGTGT CAGCCCCGGGGAAAAGCTGGAGGCCATAGCTGGGCAGCTTAAGATCCCTGCGGTGGAATTGGTCACACCG GGAGCTCAGGAGACAGAGAAGGTCACCAGTGGAGTGAAGCCGTCAGGCCTCCAGGTGTCCACCACTGGGC AGGTGGTTGCAGAGGGCCAGGAGAGTGTGCAGAGGGTGTCCACACTAGGTATCTCTTTGCCCCAGGTGGA ATTGGCCAGCTTTGGGGAGGCAGGCCCTGAGATCGTAGCCCCTTCTGCAGAGGGCACAGCAGGCTCTAGG GTCCAGGTGCCACAGGTGATGCTGGAGCTACCTGGAACCCAGGTGGCAGGGGGTGATCTGTTAGTGGGTG AGGGCATCTTCAAGATGCCCACAGTGACAGTGCCCCAGCTAGAGCTGGATGTGGGGCTGGGCCATGAAGC CCAGGCTGGTGAAGCAGCCAAGAGTGAGGGTGGGATAAAGTTGAAGTTGCCCACACTGGGGACCGGAAGC AGAGGAGAGGGCGTTGAGCCCCAGGGCCCCGAGGCCCAGCGCACCTTCCACCTCTCATTGCCCGATGTGG AACTCACGTCACCAGTGAGTAGCCACGCTGAGTACCAGGTAGTTGAGGGTGATGGGGATGGTGGGCACAA ACTCAAGGTTCGGCTGCCCCTGTTTGGTCTGGCAAAGGCCAAGGAAGGGATAGAAGTTGGAGAAAAGGCT AAGAGTCCAAAGCTCAGGCTACCCCGAGTGGGCTTCAGCCAGAGTGAGTCGGTCTCCGGAGAAGGCTCTC CAAGTCCTGAGGAGGAGGAAGAAGGCAGTGGGGAAGGGGCTTCCAGTCGCCGGGGTAGGGTAAGGGTCCG CCTGCCTCGGGTAGGCTTGGCTTCCCCTTCTAAAGTCTCTAAGGGACAGGAGGGTGATGCAACCTCCAAG TCCCCAGTTGGGGAGAAGTCACCCAAATTCCGTTTTCCTAGGGTGTCCTTAAGCCCCAAGGCCCGGAGTG GGAGTAGGGACCGGGAAGAAGGTGGATTCAGGGTCCGACTGCCCAGTGTGGGATTTTCAGAAACAGCAGT TCCAGGTTCCACCAGGATTGAGGGAACCCAGGCTGCTGCCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227574 representing NM_198048
Red=Cloning site Green=Tags(s) MEARSRSAEELRRAELVEIIVETEAQTGVSGFNVAGGGKEGIFVRELREDSPAAKSLSLQEGDQLLSARV FFENFKYEDALRLLQCAEPYKVSFCLKRTVPTGDLALRPGTVSGYEMKGPRAKVAKLNIQSLAPVKKKKM VTGALGTPADLAPVDVEFSFPKFSRLRRGLKAEAVKGPVPAAPARRRLQLPRLRVREVAEEAQVARMAAA APPPRKAKAEAEAATGAGFTAPQLELVGPRLPSAEVGVPQVSVPKGTPSTEAASGFALHLPTLGLGAPAA PAVEPPATGIQVPQVELPTLPSLPTLPTLPCLDTQEGAAVVKVPTLDVAAPSMGVDLALPGAEVEAQGEV PEVALKMPRLSFPRFGIRGKEATEAKVVKGSPEAKAKGPRLRMPTFGLSLLEPRPSGPEAVAESKLKLPT LKMPSFGIGVTGPEVKAPKGPEVKLPKVPEVKLPKVPEAAIPDVQLPEVQLPKMSDMKLPKIPEMVVPDV RLPEVQLPKVPEMKVPEMKLPKVPEMAVPDVHLPDVQLPKVPEMKLPKVPEMAVPDVHLPDVQLPKVPEM KLPEMKLPKVPEMAVPDVRLPEVQLPKVSEVKLPKMPEMAVPDVHLPELQLPKMSEVKLPKMPEMAVPDV RLPEVQLPKVSEMKLPKMPEMTMPDIRLPEVQLPKVPDIKLPEMKLPEIKLPKVPDMAVPDVPLPELQLP KVSDIRLPEMQVSQVPEVQLPKMPEMKLSKVPEVQRKSAGAEQAKGTEFSFKLPKMTMPKLGKVGKPGEA SIEVPDKLMTLPCLQPEVGTEASHVGVPSLSLPSVELDLPGALGLEGQVQEAVPGKVEKPEGPRVAVGVG EVGFRVPSVEIVTPQLPTVEVEKEQLEMVEMKVKPSSKFSLPKFGLSGPKAVKGEVEGPGRATKLKVSKF TISLPKARAGTEAEAKGAGEAGLLPALDLSIPQLSLDAQLPSGKVEVADSKPKSSRFALPKFGVKGRDSE ADVLVAGEAELEGKGWGWDGKVKMPKLKMPSFGLSRGKEAETQDGRVSPGEKLEAIAGQLKIPAVELVTP GAQETEKVTSGVKPSGLQVSTTGQVVAEGQESVQRVSTLGISLPQVELASFGEAGPEIVAPSAEGTAGSR VQVPQVMLELPGTQVAGGDLLVGEGIFKMPTVTVPQLELDVGLGHEAQAGEAAKSEGGIKLKLPTLGTGS RGEGVEPQGPEAQRTFHLSLPDVELTSPVSSHAEYQVVEGDGDGGHKLKVRLPLFGLAKAKEGIEVGEKA KSPKLRLPRVGFSQSESVSGEGSPSPEEEEEGSGEGASSRRGRVRVRLPRVGLASPSKVSKGQEGDATSK SPVGEKSPKFRFPRVSLSPKARSGSRDREEGGFRVRLPSVGFSETAVPGSTRIEGTQAAAI TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198048 |
| ORF Size | 4173 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198048.2, NP_932165.2 |
| RefSeq Size | 4668 bp |
| RefSeq ORF | 4176 bp |
| Locus ID | 19153 |
| UniProt ID | O55103 |
| Gene Summary | Scaffolding protein that functions as part of a dystroglycan complex in Schwann cells, and as part of EZR and AHNAK-containing complexes in eye lens fiber cells (PubMed:11430802, PubMed:21745462, PubMed:22764250). Required for the maintenance of the peripheral myelin sheath that is essential for normal transmission of nerve impulses and normal perception of sensory stimuli (PubMed:10839370). Required for normal transport of MBP mRNA from the perinuclear to the paranodal regions (PubMed:15356632). Required for normal remyelination after nerve injury (PubMed:10839370). Required for normal elongation of Schwann cells and normal length of the internodes between the nodes of Ranvier. The demyelinated nodes of Ranvier permit saltatory transmission of nerve impulses; shorter internodes cause slower transmission of nerve impulses (PubMed:15356632, PubMed:23022068). Required for the formation of appositions between the abaxonal surface of the myelin sheath and the Schwann cell plasma membrane; the Schwann cell cytoplasm is restricted to regions between these appositions (PubMed:15356632, PubMed:23022068). Required for the formation of Cajal bands and of Schmidt-Lanterman incisures that correspond to short, cytoplasm-filled regions on myelinated nerves (PubMed:23022068, PubMed:22764250). Recruits DRP2 to the Schwann cell plasma membrane (PubMed:11430802, PubMed:23022068, PubMed:22764250). Required for normal protein composition of the eye lens fiber cell plasma membrane and normal eye lens fiber cell morphology (PubMed:21745462).[UniProtKB/Swiss-Prot Function] |
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