Btg2 (NM_007570) Mouse Tagged ORF Clone

CAT#: MG227532

  • TrueORF®

Btg2 (tGFP-tagged) - Mouse B-cell translocation gene 2 anti-proliferative (Btg2), (10ug)

ORF Plasmid: DDK tGFP



  "NM_007570" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,800.00

CNY 2,850.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AA959598; APRO1; Pc3; TIS21
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG227532 representing NM_007570
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCACGGGAAGAGAACCGACATGCTCCCGGAGATCGCCGCCGCCGTGGGTTTCCTCTCCAGTCTCC
TGAGGACTCGGGGCTGCGTGAGCGAGCAGAGACTCAAGGTTTTCAGTAGGGCGCTCCAGGACGCACTGAC
CGATCATTACAAACACCACTGGTTTCCAGAAAAACCATCCAAAGGTTCTGGCTATCGCTGTATCCGTATC
AACCACAAGATGGACCCCATCATCAGCAAGGTGGCCAGCCAGATTGGACTCAGCCAGCCCCAGCTGCACC
GGCTGCTGCCCAGTGAGCTGACCCTGTGGGTTGATCCCTATGAAGTGTCTTACCGCATCGGGGAGGATGG
CTCCATCTGTGTCCTGTATGAGGAGGCCCCGGTGGCTGCCTCCTATGGGCTCCTCACCTGCAAGAACCAG
ATGATGCTGGGCAGGAGCAGCCCCTCGAAGAACTATGTGATGGCCGTCTCCAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG227532 representing NM_007570
Red=Cloning site Green=Tags(s)

MSHGKRTDMLPEIAAAVGFLSSLLRTRGCVSEQRLKVFSRALQDALTDHYKHHWFPEKPSKGSGYRCIRI
NHKMDPIISKVASQIGLSQPQLHRLLPSELTLWVDPYEVSYRIGEDGSICVLYEEAPVAASYGLLTCKNQ
MMLGRSSPSKNYVMAVSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007570
ORF Size 474 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007570.2, NP_031596.1
RefSeq Size 3083 bp
RefSeq ORF 477 bp
Locus ID 12227
UniProt ID Q04211
Gene Summary Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Activates mRNA deadenylation in a CNOT6 and CNOT7-dependent manner. In vitro can inhibit deadenylase activity of CNOT7 and CNOT8. Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors. Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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