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Bbc3 (NM_133234) Mouse Tagged ORF Clone
CAT＃: MG227511
- TrueORF®
Bbc3 (tGFP-tagged) - Mouse BCL2 binding component 3 (Bbc3), (10ug)
ORF Plasmid: DDK
"NM_133234" in other vectors (6)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PUMA; PUMA/JFY1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227511 representing NM_133234
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGCGCACGCCAGGAGGGCAGCTCTCCGGAGCCCGTAGAGGGTCTAGCCCGCGACAGTCCGCGCC CCTTCCCGCTCGGCCGCCTGATGCCCTCCGCTGTATCCTGCAGCCTTTGCGAGCCCGGCCTGCCCGCCGC CCCTGCTGCCCCTGCCTTGCTGCCGGCCGCCTACCTCTGCGCCCCCACCGCTCCACCTGCCGTCACCGCC GCCCTGGGGGGCCCCCGCTGGCCTGGGGGTCACCGCAGCCGGCCCAGAGGCCCGCGCCCGGACGGTCCTC AGCCCTCCCTGTCACCAGCCCAGCAGCACTTAGAGTCGCCCGTGCCCAGCGCCCCGGAGGCCCTGGCAGG AGGCCCCACCCAAGCTGCCCCGGGAGTGCGTGTGGAGGAGGAGGAGTGGGCCCGGGAGATCGGGGCCCAG CTGCGGCGGATGGCGGACGACCTCAACGCGCAGTACGAGCGGCGGAGACAAGAAGAGCAGCATCGACACC GACCCTCACCCTGGAGGGTCATGTACAATCTCTTCATGGGACTCCTCCCCTTACCCAGGGATCCTGGAGC CCCAGAAATGGAGCCCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227511 representing NM_133234
Red=Cloning site Green=Tags(s) MARARQEGSSPEPVEGLARDSPRPFPLGRLMPSAVSCSLCEPGLPAAPAAPALLPAAYLCAPTAPPAVTA ALGGPRWPGGHRSRPRGPRPDGPQPSLSPAQQHLESPVPSAPEALAGGPTQAAPGVRVEEEEWAREIGAQ LRRMADDLNAQYERRRQEEQHRHRPSPWRVMYNLFMGLLPLPRDPGAPEMEPN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133234 |
| ORF Size | 579 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133234.2, NP_573497.1 |
| RefSeq Size | 1976 bp |
| RefSeq ORF | 582 bp |
| Locus ID | 170770 |
| UniProt ID | Q99ML1 |
| Gene Summary | Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53 (By similarity). Regulates ER stress-induced neuronal apoptosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212335 | Bbc3 (untagged) - Mouse BCL2 binding component 3 (Bbc3), (10ug) |
CNY 3,990.00 |
|
| MR227511 | Bbc3 (Myc-DDK-tagged) - Mouse BCL2 binding component 3 (Bbc3) |
CNY 3,600.00 |
|
| MR227511L1 | Lenti ORF clone of Bbc3 (Myc-DDK-tagged) - Mouse BCL2 binding component 3 (Bbc3) |
CNY 4,750.00 |
|
| MR227511L2 | Lenti ORF clone of Bbc3 (mGFP-tagged) - Mouse BCL2 binding component 3 (Bbc3) |
CNY 6,000.00 |
|
| MR227511L3 | Lenti ORF clone of Bbc3 (Myc-DDK-tagged) - Mouse BCL2 binding component 3 (Bbc3) |
CNY 4,750.00 |
|
| MR227511L4 | Lenti ORF clone of Bbc3 (mGFP-tagged) - Mouse BCL2 binding component 3 (Bbc3) |
CNY 4,750.00 |
