Tbx20 (NM_020496) Mouse Tagged ORF Clone

CAT＃: MG227295

TrueORF^®

Tbx20 (tGFP-tagged) - Mouse T-box 20 (Tbx20) transcript variant 2, (10ug)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_020496" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,230.00

货期*

4周

规格

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA363076

TBX20 Antibody - middle region

CNY 4,628.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	9430010M06Rik; AL022859; Tbx12
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG227295 representing NM_020496 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCACGGCGTCGCCCAAGCCCCAGCTCTCCTCTCGAGCCAATGCCTTCTCCATCGCCGCGCTTA TGTCCAGCGGCGGCCCCAAGGAGAAGGAGGCAGCAGAGAACACCATCAAACCCCTGGAACAATTTGTGGA GAAATCATCATGTGCCCAGCCACTGGGTGAGCTGACGAGTCTGGATGCTCACGCGGAGTTTGGCGGCGGG GGCGGCAGCCCATCCTCATCCTCTCTGTGCACAGAGCCACTGATACCCACCACCCCCATCATCCCCAGCG AAGAGATGGCTAAAATCGCCTGCAGCCTGGAAACGAAGGAGCTCTGGGACAAATTCCATGAACTGGGCAC GGAGATGATAATCACCAAGTCTGGCAGGAGGATGTTCCCCACCATCCGCGTGTCATTTTCTGGAGTGGAT CCTGAGTCCAAGTATATAGTCCTGATGGACATCGTCCCGGTGGACAACAAGAGATACCGCTATGCCTACC ACCGGTCATCCTGGCTGGTGGCTGGCAAAGCTGATCCCCCGCTGCCAGCCAGGCTCTACGTGCACCCAGA CTCCCCCTTTACTGGCGAGCAGCTCCTCAAACAGATGGTGTCTTTTGAAAAGGTGAAGCTCACCAACAAT GAACTGGATCAACACGGCCATATAATTTTGAATTCAATGCATAAGTACCAGCCACGGGTGCACATCATAA AGAAGAAAGACCACACGGCCTCCTTGCTCAATCTGAAGTCAGAAGAATTCAGGACGTTCATCTTTCCAGA GACAGTTTTCACAGCAGTCACAGCCTACCAGAACCAACTGATAACCAAGCTGAAAATAGACAGCAATCCG TTTGCCAAAGGATTCCGGGACTCCTCCAGGCTCACTGACATTGAGAGGGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG227295 representing NM_020496 Red=Cloning site Green=Tags(s) MEFTASPKPQLSSRANAFSIAALMSSGGPKEKEAAENTIKPLEQFVEKSSCAQPLGELTSLDAHAEFGGG GGSPSSSSLCTEPLIPTTPIIPSEEMAKIACSLETKELWDKFHELGTEMIITKSGRRMFPTIRVSFSGVD PESKYIVLMDIVPVDNKRYRYAYHRSSWLVAGKADPPLPARLYVHPDSPFTGEQLLKQMVSFEKVKLTNN ELDQHGHIILNSMHKYQPRVHIIKKKDHTASLLNLKSEEFRTFIFPETVFTAVTAYQNQLITKLKIDSNP FAKGFRDSSRLTDIERE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_020496
ORF Size	891 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020496.3, NP_065242.1
RefSeq Size	1800 bp
RefSeq ORF	894 bp
Locus ID	57246
UniProt ID	Q9ES03
Gene Summary	Acts as a transcriptional activator and repressor required for cardiac development and may have key roles in the maintenance of functional and structural phenotypes in adult heart.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC210116	Tbx20 (untagged) - Mouse T-box 20 (Tbx20), transcript variant 2, (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,990.00
MR227295	Tbx20 (Myc-DDK-tagged) - Mouse T-box 20 (Tbx20), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,950.00
MR227295L3	Lenti ORF clone of Tbx20 (Myc-DDK-tagged) - Mouse T-box 20 (Tbx20), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,850.00
MR227295L4	Lenti ORF clone of Tbx20 (mGFP-tagged) - Mouse T-box 20 (Tbx20), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,850.00

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