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Asic3 (NM_183000) Mouse Tagged ORF Clone
CAT＃: MG227272
- TrueORF®
Asic3 (tGFP-tagged) - Mouse amiloride-sensitive cation channel 3 (Accn3), (10ug)
ORF Plasmid: DDK
"NM_183000" in other vectors (4)
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CNY 5,544.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Accn3; AW742291; DRASIC; SLNAC1; TNAC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227272 representing NM_183000
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACCTCCCTCAGGACTGGAGGAGGCCCAGCGGCGACAGGCCTCAGACATCCGGGTGTTCGCCAACA GCTGCACGATGCATGGTTTGGGCCACATCTTTGGCCCTGGAGGCCTGACCCTGCGCCGTGGGCTGTGGGC CACAGCTGTACTCCTGTCGCTGGCGGCCTTCCTCTACCAGGTGGCTGAGCGGGTTCGCTACTATGGGGAG TTCCACCATAAGACCACCCTGGATGAGCGTGAGAGCCACCAGCTTACCTTCCCAGCTGTCACTTTGTGTA ACATCAATCCCCTGCGCCGCTCACGCCTCACACCCAATGACTTGCACTGGGCCGGAACGGCACTGCTGGG TCTGGACCCTGCTGAACATGCTGCCTACCTTCGTGCCCTGGGCCAGCCCCCTGCACCACCTGGCTTCATG CCCAGTCCGACTTTTGACATGGCACAACTCTACGCCAGAGCCGGCCACTCCCTTGAGGACATGTTGCTGG ACTGCCGATACCGTGGCCAGCCCTGTGGACCTGAGAACTTCACAGTGATTTTCACTCGAATGGGGCAATG CTACACCTTCAACTCTGGTGCCCAGGGGGCAGAGCTGCTCACCACTCCTAAGGGCGGTGCTGGCAATGGA CTGGAGATTATGCTGGATGTACAGCAGGAGGAGTATCTGCCCATCTGGAAGGACATGGAAGAGACCCCAT TTGAGGTGGGGATCCGAGTGCAGATCCACGGCCAGGAGGAACCCCCTGCCATTGACCAGCTGGGCTTCGG TGCTGCCCCAGGCCACCAGACTTTTGTGTCCTGCCAGCAACAGCAACTGAGTTTCCTGCCACCACCCTGG GGTGACTGCAATACCGCATCTGTGGATCCCGACTTTGATCCAGAGCCCTCTGATCCCCTGGGTTCCCCTA GCTCCAGCCCTCCTTATAGCTTAATAGGGTGTCGCCTGGCCTGTGAGTCACGCTATGTGGCTCGGAAGTG CGGATGTCGAATGATGCATATGCCTGGAAACTCCCCAGTGTGCAGCCCCCAGCAGTACAAGGACTGTGCC AGCCCAGCTCTGGACGCTATGCTGCGAAAGGACACTTGTGTCTGTCCCAACCCGTGCGCCACTACACGCT ATGCCAAGGAGCTCTCCATGGTGCGGATTCCCAGCCGCGCTTCAGCTCGCTACCTGGCCCGGAAATACAA CCGTAGCGAGACTTACATCACGGAGAATGTACTGGTTCTGGATATCTTCTTTGAAGCCCTCAACTATGAG GCCGTGGAACAAAAGGCAGCTTATGAAGTGTCGGAGTTGCTGGGAGACATTGGGGGACAGATGGGACTGT TTATCGGAGCCAGCCTGCTTACCATCCTCGAGATCCTCGACTACCTCTGTGAGGTTTTTCAAGACAGAGT CCTGGGGTACTTCTGGAACAGAAGGAGCTCTCAAAGGCGCTCTGGCAACACTCTGCTCCAGGAAGAGTTG AATGGGCATCGAACACATGTTCCCCATCTCAGCCTAGGCCCCAGGCCTCCTACCGCTCCCAGTGCTGTCA CCAAGACACTCGCTGCCTCCCACCGTACCTGTTACCTCGTGACAAGGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227272 representing NM_183000
Red=Cloning site Green=Tags(s) MKPPSGLEEAQRRQASDIRVFANSCTMHGLGHIFGPGGLTLRRGLWATAVLLSLAAFLYQVAERVRYYGE FHHKTTLDERESHQLTFPAVTLCNINPLRRSRLTPNDLHWAGTALLGLDPAEHAAYLRALGQPPAPPGFM PSPTFDMAQLYARAGHSLEDMLLDCRYRGQPCGPENFTVIFTRMGQCYTFNSGAQGAELLTTPKGGAGNG LEIMLDVQQEEYLPIWKDMEETPFEVGIRVQIHGQEEPPAIDQLGFGAAPGHQTFVSCQQQQLSFLPPPW GDCNTASVDPDFDPEPSDPLGSPSSSPPYSLIGCRLACESRYVARKCGCRMMHMPGNSPVCSPQQYKDCA SPALDAMLRKDTCVCPNPCATTRYAKELSMVRIPSRASARYLARKYNRSETYITENVLVLDIFFEALNYE AVEQKAAYEVSELLGDIGGQMGLFIGASLLTILEILDYLCEVFQDRVLGYFWNRRSSQRRSGNTLLQEEL NGHRTHVPHLSLGPRPPTAPSAVTKTLAASHRTCYLVTRL TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_183000 |
| ORF Size | 1590 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_183000.2, NP_892045.2 |
| RefSeq Size | 1978 bp |
| RefSeq ORF | 1593 bp |
| Locus ID | 171209 |
| UniProt ID | Q6X1Y6 |
| Gene Summary | Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217635 | Asic3 (untagged) - Mouse amiloride-sensitive cation channel 3 (Accn3), (10ug) |
CNY 6,840.00 |
|
| MR227272 | Asic3 (Myc-DDK-tagged) - Mouse amiloride-sensitive cation channel 3 (Accn3) |
CNY 3,944.00 |
|
| MR227272L3 | Lenti ORF clone of Asic3 (Myc-DDK-tagged) - Mouse amiloride-sensitive cation channel 3 (Accn3) |
CNY 6,344.00 |
|
| MR227272L4 | Lenti ORF clone of Asic3 (mGFP-tagged) - Mouse amiloride-sensitive cation channel 3 (Accn3) |
CNY 6,344.00 |
