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Best1 (NM_011913) Mouse Tagged ORF Clone
CAT＃: MG227104
- TrueORF®
Best1 (tGFP-tagged) - Mouse bestrophin 1 (Best1), (10ug)
ORF Plasmid: DDK
"NM_011913" in other vectors (4)
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CNY 6,080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Bmd; mBest1; Vmd2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227104 representing NM_011913
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTATCACCTACACAAACAAAGTAGCCAATGCCCGCCTCGGTTCGTTCTCGTCCCTCCTCCTGTGCT GGCGAGGCAGCATCTACAAGCTGCTGTATGGAGAATTCCTTGTCTTCATATTCCTCTACTATTCCATCCG TGGACTCTACAGAATGGTTCTCTCGAGTGATCAGCAGCTGTTGTTTGAGAAGCTGGCTCTGTACTGCGAC AGCTACATTCAGCTCATCCCTATATCCTTCGTTCTGGGTTTCTATGTTACATTGGTGGTGAGCCGCTGGT GGAGCCAGTACGAGAACTTGCCGTGGCCCGACCGCCTCATGATCCAGGTGTCTAGCTTCGTGGAGGGCAA GGATGAGGAAGGCCGTTTGCTGCGGCGCACGCTCATCCGCTACGCCATCCTGGGCCAAGTGCTCATCCTG CGCAGCATCAGCACCTCGGTCTACAAGCGCTTTCCCACTCTTCACCACCTGGTGCTAGCAGGTTTTATGA CCCATGGGGAACATAAGCAGTTGCAGAAGTTGGGCCTACCACACAACACATTCTGGGTGCCCTGGGTGTG GTTTGCCAACTTGTCAATGAAGGCCTATCTTGGAGGTCGAATCCGGGACACCGTCCTGCTCCAGAGCCTG ATGAATGAGGTGTGTACTTTGCGTACTCAGTGTGGACAGCTGTATGCCTACGACTGGATAAGTATCCCAT TGGTGTACACACAGGTGGTGACAGTGGCAGTATACAGCTTTTTCCTTGCATGCTTGATCGGGAGGCAGTT TCTGAACCCAAACAAGGACTACCCAGGCCATGAGATGGATCTGGTTGTGCCTGTCTTCACAATCCTGCAA TTCTTATTCTACATGGGCTGGCTGAAGGTGGCAGAACAGCTCATCAACCCCTTCGGGGAGGACGATGATG ATTTTGAGACTAACTGGATCATTGACAGAAACCTGCAGGTGTCCCTGTTGTCCGTGGATGGGATGCACCA GAACTTGCCTCCCATGGAACGTGACATGTACTGGAACGAGGCAGCGCCTCAGCCGCCCTACACAGCTGCT TCTGCCAGGTCTCGCCGGCATTCCTTCATGGGCTCCACCTTCAACATCAGCCTAAAGAAAGAAGACTTAG AGCTTTGGTCAAAAGAGGAGGCTGACACGGATAAGAAAGAGAGTGGCTATAGCAGCACCATAGGCTGCTT CTTAGGACTGCAACCCAAAAACTACCATCTTCCCTTGAAAGACTTAAAGACCAAACTATTGTGTTCTAAG AACCCCCTCCTCGAAGGCCAGTGTAAGGATGCCAACCAGAAAAACCAGAAAGATGTCTGGAAATTTAAGG GTCTGGACTTCTTGAAATGTGTTCCAAGGTTTAAGAGGAGAGGCTCCCATTGTGGCCCACAGGCACCCAG CAGCCACCCTACTGAGCAGTCAGCACCCTCCAGTTCAGACACAGGTGATGGGCCTTCCACAGATTACCAA GAAATCTGTCACATGAAAAAGAAAACTGTGGAGTTTAACTTGAACATTCCAGAGAGCCCCACAGAACATC TTCAACAGCGCCGTTTGGACCAGATGTCAACCAATATACAGGCTCTAATGAAGGAGCATGCAGAGTCCTA TCCCTACAGGGATGAAGCTGGCACCAAACCTGTTCTCTATGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227104 representing NM_011913
Red=Cloning site Green=Tags(s) MTITYTNKVANARLGSFSSLLLCWRGSIYKLLYGEFLVFIFLYYSIRGLYRMVLSSDQQLLFEKLALYCD SYIQLIPISFVLGFYVTLVVSRWWSQYENLPWPDRLMIQVSSFVEGKDEEGRLLRRTLIRYAILGQVLIL RSISTSVYKRFPTLHHLVLAGFMTHGEHKQLQKLGLPHNTFWVPWVWFANLSMKAYLGGRIRDTVLLQSL MNEVCTLRTQCGQLYAYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLNPNKDYPGHEMDLVVPVFTILQ FLFYMGWLKVAEQLINPFGEDDDDFETNWIIDRNLQVSLLSVDGMHQNLPPMERDMYWNEAAPQPPYTAA SARSRRHSFMGSTFNISLKKEDLELWSKEEADTDKKESGYSSTIGCFLGLQPKNYHLPLKDLKTKLLCSK NPLLEGQCKDANQKNQKDVWKFKGLDFLKCVPRFKRRGSHCGPQAPSSHPTEQSAPSSSDTGDGPSTDYQ EICHMKKKTVEFNLNIPESPTEHLQQRRLDQMSTNIQALMKEHAESYPYRDEAGTKPVLYE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011913 |
| ORF Size | 1653 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011913.2, NP_036043.2 |
| RefSeq Size | 2087 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 24115 |
| UniProt ID | O88870 |
| Gene Summary | Forms calcium-sensitive chloride channels. Permeable to bicarbonate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218825 | Best1 (untagged) - Mouse bestrophin 1 (Best1), (10ug) |
CNY 7,030.00 |
|
| MR227104 | Best1 (Myc-DDK-tagged) - Mouse bestrophin 1 (Best1) |
CNY 6,152.00 |
|
| MR227104L3 | Lenti ORF clone of Best1 (Myc-DDK-tagged) - Mouse bestrophin 1 (Best1) |
CNY 7,410.00 |
|
| MR227104L4 | Lenti ORF clone of Best1 (mGFP-tagged) - Mouse bestrophin 1 (Best1) |
CNY 7,410.00 |
