Mitf (NM_008601) Mouse Tagged ORF Clone
CAT#: MG227084
- TrueORF®
Mitf (tGFP-tagged) - Mouse microphthalmia-associated transcription factor (Mitf) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_008601" in other vectors (4)
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CNY 4,560.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BCC2; Bhlhe32; bw; Gsfbcc2; mi; vit; Vitiligo; Wh |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227084 representing NM_008601
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGAAATGCTAGAATACAGTCACTACCAGGTGCAGACCCACCTGGAAAACCCCACCAAGTACCACA TACAGCAAGCTCAGAGGCACCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATGCCAGCCA AGTCCTGAGCTCACCATGTCCAAACCAGCCTGGCGACCATGCCATGCCACCAGTGCCGGGGAGCAGCGCA CCCAACAGCCCTATGGCTATGCTCACTCTTAACTCCAACTGTGAAAAAGAGGCATTTTATAAGTTTGAGG AGCAGAGCAGGGCAGAGAGTGAGTGCCCAGGTATGAACACGCACTCTCGAGCGTCGTGCATGCAGATGGA TGATGTAATTGATGACATCATCAGCCTGGAATCAAGTTATAATGAAGAAATTTTGGGCTTGATGGATCCG GCCTTGCAAATGGCAAATACGTTACCCGTCTCTGGAAACTTGATCGACCTCTACAGCAACCAGGGCCTGC CACCGCCAGGCCTTACCATCAGCAACTCCTGTCCAGCCAACCTTCCCAACATAAAAAGGGAGCTCACAGC GTGTATTTTCCCCACAGAGTCTGAAGCAAGAGCATTGGCTAAAGAGAGGCAGAAAAAGGACAATCACAAC TTGATTGAACGAAGAAGAAGATTTAACATAAACGACCGCATTAAGGAGCTAGGTACTCTGATCCCCAAGT CAAATGATCCAGACATGCGGTGGAACAAGGGAACCATTCTCAAGGCCTCTGTGGACTACATCCGGAAGTT GCAACGGGAACAGCAACGAGCTAAGGACCTTGAAAACCGACAGAAGAAGCTGGAGCATGCGAACCGGCAC CTGCTGCTCAGAGTACAGGAGCTGGAGATGCAGGCTAGAGCGCATGGACTTTCCCTTATCCCATCCACCG GTCTCTGCTCGCCTGATCTGGTGAATCGGATCATCAAGCAAGAACCAGTTCTTGAGAACTGCAGCCAGGA ACTTGTACAGCACCAGGCAGACCTGACATGTACGACAACTCTGGATCTCACGGACGGTACCATCACCTTT ACCAACAACCTCGGCACCATGCCGGAGAGCAGCCCGGCCTACAGCATCCCCAGGAAGATGGGCTCCAACT TGGAAGACATCCTGATGGACGATGCCCTCTCACCTGTTGGAGTCACCGACCCACTGCTGTCATCAGTGTC GCCAGGAGCTTCAAAAACAAGCAGCCGGAGGAGCAGTATGAGCGCAGAAGAAACGGAGCATGCGTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227084 representing NM_008601
Red=Cloning site Green=Tags(s) MLEMLEYSHYQVQTHLENPTKYHIQQAQRHQVKQYLSTTLANKHASQVLSSPCPNQPGDHAMPPVPGSSA PNSPMAMLTLNSNCEKEAFYKFEEQSRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDP ALQMANTLPVSGNLIDLYSNQGLPPPGLTISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHN LIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKDLENRQKKLEHANRH LLLRVQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQELVQHQADLTCTTTLDLTDGTITF TNNLGTMPESSPAYSIPRKMGSNLEDILMDDALSPVGVTDPLLSSVSPGASKTSSRRSSMSAEETEHAC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008601 |
ORF Size | 1257 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008601.3, NP_032627.1 |
RefSeq Size | 4581 bp |
RefSeq ORF | 1260 bp |
Locus ID | 17342 |
UniProt ID | Q08874 |
Gene Summary | This transcription factor serves at a critical point between extracellular signaling and downstream targets in cell specification in early eye and neural crest development. Mutant alleles have been identified that generate distinct phenotypes. Some of these alleles are being used to model the human diseases Waardenburg syndrome IIa and Tietz syndrome. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208963 | Mitf (untagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 2, (10ug) |
CNY 4,024.00 |
|
MR227084 | Mitf (Myc-DDK-tagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 2 |
CNY 5,856.00 |
|
MR227084L3 | Lenti ORF clone of Mitf (Myc-DDK-tagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 2 |
CNY 6,080.00 |
|
MR227084L4 | Lenti ORF clone of Mitf (mGFP-tagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 2 |
CNY 8,256.00 |