Htr1a (NM_008308) Mouse Tagged ORF Clone
CAT#: MG227083
- TrueORF®
Htr1a (tGFP-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a), (10ug)
ORF Plasmid: DDK
"NM_008308" in other vectors (4)
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CNY 7,088.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Gpcr18 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227083 representing NM_008308
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATGTTCAGTCTTGGCCAGGGCAACAACACCACAACGTCCCTGGAGCCCTTCGGGACAGGCGGCA ACGATACTGGCCTCTCCAACGTGACCTTCAGCTACCAAGTGATCACCTCTCTTTTGCTGGGGACGCTCAT TTTCTGCGCGGTGCTCGGCAATGCCTGCGTGGTTGCTGCCATCGCCCTGGAGCGCTCCCTTCAGAATGTT GCCAACTATCTCATCGGCTCCTTGGCGGTCACCGATCTCATGGTGTCAGTGCTGGTGCTGCCCATGGCTG CTCTGTACCAGGTGCTCAACAAGTGGACTCTGGGTCAGGTCACCTGTGACCTGTTTATCGCCCTGGATGT GCTGTGCTGCACTTCGTCCATCCTGCACCTGTGCGCCATCGCGCTAGACAGGTACTGGGCAATCACCGAC CCTATAGACTACGTGAACAAGAGGACGCCCCGGCGCGCCGCTGCGCTGATCTCGCTCACTTGGCTCATTG GCTTTCTCATCTCCATCCCGCCTATGCTGGGCTGGCGCACCCCGGAAGACCGCTCGAACCCCAACGAGTG CACCATCAGCAAGGACCACGGGTACACCATCTACTCCACTTTCGGCGCTTTCTATATTCCGCTGCTGCTC ATGCTGGTCCTCTATGGGCGCATCTTCAGAGCCGCGCGCTTCCGAATCCGCAAGACGGTCAAGAAGGTGG AAAAGAAGGGAGCGGGCACCAGCTTCGGAACATCGTCGGCCCCGCCCCCCAAGAAGAGCCTGAATGGTCA GCCAGGTAGTGGGGACTGCAGGCGCAGTGCTGAGAACAGGGCGGTGGGGACTCCGTGCGCTAATGGGGCG GTGAGACAGGGTGAGGACGACGCCACCCTGGAGGTGATCGAGGTGCATCGAGTGGGCAACTCCAAAGGGC ACCTTCCTCTGCCCAGCGAATCAGGAGCTACCTCCTATGTCCCCGCCTGCTTGGAGAGAAAAAATGAGCG CACTGCTGAGGCAAAGCGCAAGATGGCCTTGGCCCGTGAGAGGAAGACAGTGAAGACACTGGGCATCATC ATGGGCACCTTCATCCTCTGCTGGCTGCCCTTTTTCATTGTGGCCCTGGTCCTACCTTTCTGTGAGAGCA GTTGCCACATGCCTGAGTTGTTGGGTGCCATAATTAACTGGCTAGGCTACTCCAACTCCCTGCTCAACCC AGTTATTTATGCTTATTTCAACAAAGACTTTCAAAACGCTTTTAAGAAGATCATCAAGTGCAAGTTCTGC CGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227083 representing NM_008308
Red=Cloning site Green=Tags(s) MDMFSLGQGNNTTTSLEPFGTGGNDTGLSNVTFSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNV ANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITD PIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSNPNECTISKDHGYTIYSTFGAFYIPLLL MLVLYGRIFRAARFRIRKTVKKVEKKGAGTSFGTSSAPPPKKSLNGQPGSGDCRRSAENRAVGTPCANGA VRQGEDDATLEVIEVHRVGNSKGHLPLPSESGATSYVPACLERKNERTAEAKRKMALARERKTVKTLGII MGTFILCWLPFFIVALVLPFCESSCHMPELLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFC R TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008308 |
ORF Size | 1263 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008308.4, NP_032334.2 |
RefSeq Size | 4441 bp |
RefSeq ORF | 1266 bp |
Locus ID | 15550 |
UniProt ID | Q64264 |
Gene Summary | G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208723 | Htr1a (untagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a), (10ug) |
CNY 5,488.00 |
|
MR227083 | Htr1a (Myc-DDK-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a) |
CNY 5,488.00 |
|
MR227083L3 | Lenti ORF clone of Htr1a (Myc-DDK-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a) |
CNY 6,080.00 |
|
MR227083L4 | Lenti ORF clone of Htr1a (mGFP-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 1A (Htr1a) |
CNY 6,080.00 |