Mki67 (NM_001081117) Mouse Tagged ORF Clone
CAT#: MG227041
- TrueORF®
Mki67 (tGFP-tagged) - Mouse antigen identified by monoclonal antibody Ki 67 (Mki67), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001081117" in other vectors (2)
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CNY 28,220.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D630048A14Rik; Ki-67; Ki67 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227041 representing NM_001081117, codon optimized.
Due to the complexity of NM_001081117, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCATCTGCCCACCTGGTAACAATAAAGAGGTCCGGCGACGACGGAGCCCATTTTCCTCTGTCTC TGAGTAGCTGCCTCTTCGGCAGGTCCATTGAGTGCGACATTAGAATCCAGCTGCCCGTGGTGAGCAAGAG GCACTGCAAGATAGAAGTGAAGGAACAGGAAGCCATCCTGTACAACTTTTCTTCAACAAACCCCACCCAG GTAAACGGCGTGACAATCGATGAGCCTGTAAGGCTGAGGCACGGCGATATCATCACCATAATCGACCGGT CCTTCAGATACGAAGATGGTAACCATGAAGACGGGTCTAAGCCTACCGAGTTCCCAGGCAAAAGCTTGGG TAAAGAACCCAGCCGGCGCGCTAGTAGGGATAGTTTTTGCGCGGACCCGGATGGCGAAGGTCAGGATACA AAAGCTAGCAAGATGACCGCCTCCCGAAGATCATTTGTGTATGCGAAAGGGCTCAGTGCAGACTCCCCAG CTTCCGACGGAAGTAAAAACAGTGTGTCTCAGGACTCATCTGGTCACGTGGAACAGCACACTGGCAGGAA CATCGTGGAGCCGACATCTGGGGATCTGTTCAAGAAGTCCCGGTCAACAGGTTCAAGCTACCGAGAGCCA AAATCTTCTCCTACTCAGAGCTTGTCTAATTCTAACGAAAAGGAATCTCCCTTCGAGAAGCTCTACCAGT CTATGAAAGAGGAGCTGGATGTCAAGTCTCAGAAGAGTTGCCGAAAGAGCGAACCCCAGCCGGATAGAGC AGCCGAGGAGAGCCGCGAGACTCAGCTGCTCGTGAGCGGCAGGGCCAGAGCGAAGTCATCAGGATCTACC CCTGTGACTGCCGCATCTTCCCCAAAAGTGGGAAAGATCTGGACTGAGCGCTGGAGGGGGGGGATGGTCC CAGTCCAGACCTCTACCGAAACAGCAAAGATGAAGACCCCAGTCCGCCACTCTCAGCAGCTCAAAGACGA GGATTCTAGAGTCACAGGGAGAAGGCACAGTGTCAACCTGGACGAGGGGGAGTCCGCACAGGCTGTGCAC AAGACAGTCACACCGGGTAAGCTGGCGACTAGGAATCAGGCAGCCGTTGAGGCTGGGGACGTGGCATCCC CCGCTGACACTCCCGAGCATTCAAGTTCCAAAAAGAGATCTATTCCCGCCAAGGTGGAAGCTCCTTCCGC CGAGACACAAAAGCGCCTGTCTCTCACGCAGCGCCTGGTACCCGGGGAGAAGAAGACACCAAAGGGCTCT TTCAGTAAGCCAGAGAAGCTGGCCACCGCAGCCGAACAGACCTGTTCCGGTCTGCCTGGGCTGAGCAGCG TGGACATTTCTAACTTCGGAGACAGTATCAACAAGAGCGAGGGAATGCCAATGAAGCGACGGAGGGTGAG CTTCGGTGGCCATCTGCGACCTGAGTTGTTCGATGAGAATCTTCCTCCTAACACCCCACTGAAGCGGGGC GAAACACCGACCAAACGGAAAAGCCTCGGGACCCACAGTCCCGCTGTGCTGAAGACGATCATCAAGGAAC GACCGCAAAGCCCTGGCAAACAGGAAAGCCCTGGTATTACTCCCCCAAGGACGAATGACCAGCGCAGGAG GAGCGGTCGGACCAGTAGTGGTTCTAAGTTCTTGTGTGAAACAGATATACCGAAGAAAGCCGGTAGGAAG AGTGGTAACCTCCCAGCAAAAAGAGCTTCTATCAGCCGGTCCCAGCACGGGATCCTCCAGATGATTTGTA GCAAGAGGCGCTCTGGCGCCAGCGAGGCCAACTTGATCGTCGCCAAGTCCTGGGCAGATGTTGTGAAGCT CGGGGTTAAACAGACCCAGACAAAGGTCGCGAAACACGTGCCCCAAAAACAGACTTCTAAGAGGCAGCGC AGGCCAAGTACACCAAAGAAGCCAACAAGCAATCTGCACAACCAGTTCACAACTGGCCACGCCAACTCTC CGTGCACTATCGTGGTGGGTAGAGCACAGATCGAGAAGGTGTCAGTGCCTGCAAGGCCTTATAAAATGCT GAACAACCTGATGCTCAATAGGAAAGTAGACTTCTCCGAAGACCTGAGCGGCCTGACCGAAATGTTCAAG ACACCCGTTAAGGAGAAGCAGCAGCAGATGTCCGACACAGGATCAGTGCTGTCAAATTCCGCCAACCTTT CTGAAAGACAGTTGCAAGTAACAAATTCTGGGGACATACCGGAGCCCATCACAACTGAAATCCTCGGCGA AAAAGTACTTTCAAGTACTCGAAATGCAGCGAAGCAACAATCAGATCGGTATAGCGCCAGCCCGACACTG CGGCGCCGATCAATAAAGCATGAGAATACCGTGCAGACCCCCAAGAACGTTCACAACATCACAGACCTTG AAAAGAAGACACCCGTCTCCGAAACAGAGCCACTTAAAACGGCTTCATCCGTGAGTAAGCTGCGCCGGAG CAGGGAACTTCGCCATACACTTGTGGAAACGATGAACGAGAAGACAGAGGCTGTGCTGGCTGAAAATACT ACCGCGCGACATCTGAGAGGGACATTTCGCGAGCAGAAGGTGGACCAACAAGTCCAGGACAACGAAAATG CCCCACAGCGGTGCAAGGAGTCTGGTGAGTTGTCAGAGGGAAGTGAGAAGACTAGTGCCAGACGCTCCTC TGCTCGCAAGCAGAAACCAACTAAGGACCTCCTGGGGAGCCAGATGGTCACGCAGACAGCTGATTACGCA GAGGAACTGCTGAGTCAGGGCCAGGGGACAATTCAGAATCTCGAGGAGTCCATGCATATGCAGAACACCT CTATCTCAGAGGATCAGGGGATAACAGAGAAGAAAGTGAACATCATCGTGTATGCTACTAAGGAAAAGCA CAGTCCAAAGACGCCAGGGAAAAAGGCGCAGCCTCTTGAAGGTCCCGCCGGATTGAAAGAGCACTTCGAG ACGCCGAACCCTAAAGACAAGCCTATCACCGAGGATCGCACCCGGGTCCTGTGTAAAAGCCCGCAGGTGA CAACCGAGAACATTACCACGAACACTAAGCCGCAGACATCTACAAGCGGCAAGAAGGTGGATATGAAGGA AGAATCCTCCGCACTGACGAAACGAATCCATATGCCCGGGGAGTCTCGACACAACCCCAAAATACTGAAG TTGGAGTGCGAAGATATAAAGGCTCTGAAGCAATCCGAAAATGAGATGCTGACCAGTACAGTGAATGGAT CAAAGCGAACCCTGGAAAAAAGCAAGAAAAAAGCTCAGCCCCTTGAGGACCTCACCTGCTTCCAGGAACT GTTCATCTCTCCCGTGCCAACTAACATCATAAAGAAGATTCCTTCCAAATCCCCACACACTCAGCCGGTG CGGACTCCTGCTAGCACAAAGAGGCTGTCCAAGACTGGCTTGAGTAAGGTGGATGTACGCCAGGAACCAA GCACTCTGGGAAAACGGACAAAGAGCCCTGGTAGGGCACCCGGTACTCCCGCCCCCGTGCAAGAGGAGAA CGATAGCACAGCTTTCATGGAAACGCCTAAGCAAAAACTGGACTTCGCAGGTAACAGCAGCGGCTCCAAA CGGAGATCTCGAACTTCAAAGAACAGAAGTCAACCCTTGGAGGACCTCGATGGCTTCCAGGAGCTGTTCC AGACGCCGGCCGGAGCATCTGACTCCGTGACCGTCGAGGAGTCAGCCAAAATAAGTCTCGAGAGTTCCCA AGCTGAACCGGTTAAGACTCCCGCTAGCACAAAGAGAAGATCTAAAATGTCTCTGATGAAAGTGGACATG AAGGAGCTGAGCATACTCGAAAAGCAAACGCAAAGTCGGGGTCGAGATGCAGGAACGCCAGCGCCCATGC AGGAAGGCAATGGAACAACAGCAATAATGGAGACACCTAAACAGAAGCTGGATTTTACCGGGAACAGCAC AGGACATAAGAGGAGGCCAAGAACGCCCAAAATCAGAGCTCAACCCCTGGAGGATCTTGATGGATTCCAG GAGCTGTTCCAAACCCCCGCAGGTGCTAACGACTCTGTGACAGTTGAAGAGTCTGCCAAGATGAGTCTTG AGAGTAGTCAGGCAGAACCAGTGAAGACACCAGCCTCAACCAAACGCTTGAGTAAAACTGATCTCAGTAA AGTGGACGTGCGAGAAGATCCCAGTATCCTGGGGAAGAAGACAAAGTCTCCCGGGAGGGCTCCAGGCACA CCAGCCCCAGTTCAGGAAGAGAACGACTGTACCGCTTACATGGAGACTCCCAAGCAGAAACTGGAGTCCA TAGAAAACCTGACAGGCCTTAGGAAGCAGTCAAGGACACCAAAGGACATCACCGGATTCCAAGATTCATT CCAGATCCCTGACCATGCAAATGGCCCTCTGGTCGTAGTCAAGACCAAGAAGATGTTCTTTAACTCACCA CAGCCAGAATCCGCGATAACACGCAAATCACGGGAAAGGCAGAGCAGGGCGAGTATCAGTAAGATAGATG TAAAAGAAGAGCTGCTCGAATCTGAAGAACATCTTCAGCTGGGCGAGGGCGTCGACACCTTTCAGGTGTC TACTAACAAGGTTATCCGGTCCTCCAGGAAACCTGCTAAAAGGAAGCTGGACAGTACTGCCGGTATGCCT AATTCTAAGAGGATGAGATGCTCCTCCAAGGATAACACCCCTTGTCTTGAAGATCTGAATGGGTTCCAGG AACTTTTCCAGATGCCAGGCTACGCTAATGACAGTCTTACGACCGGCATCTCTACGATGCTGGCGCGCTC ACCCCAGCTCGGTCCTGTAAGGACTCAGATTAACAAGAAGAGCCTTCCAAAAATCATTCTGCGCAAGATG GACGTGACCGAGGAGATTAGTGGTCTGTGGAAGCAGTCCCTTGGCAGGGTTCACACTACCCAGGAACAGG AGGACAACGCTATCAAAGCCATCATGGAAATTCCTAAAGAGACTCTCCAGACTGCAGCAGACGGCACTCG GCTGACCAGGCAGCCCCAGACCCCCAAAGAGAAAGTCCAACCTCTCGAGGACCACAGCGTGTTCCAGGAA CTCTTTCAGACTAGCCGATACTGCTCCGACCCTTTGATTGGTAATAAGCAGACTAGGATGAGCTTGCGCT CCCCCCAGCCTGGATTCGTGAGGACCCCACGCACCTCAAAAAGACTCGCCAAAACTTCTGTAGGCAACAT TGCCGTGAGAGAGAAAATATCTCCCGTGTCACTTCCCCAGTGCGCCACGGGTGAGGTAGTCCACATCCCC ATAGGCCCCGAAGATGATACGGAGAACAAGGGCGTTAAGGAATCAACACCACAGACTTTGGACTCATCCG CTAGTAGGACAGTGTCAAAGCGACAACAGGGAGCCCACGAGGAAAGACCACAATTCAGTGGGGACCTGTT CCATCCGCAAGAACTCTTTCAGACGCCCGCCTCAGGCAAAGATCCAGTGACCGTGGACGAAACAACGAAA ATCGCCCTTCAGTCCCCCCAGCCAGGACACATTATAAATCCAGCAAGTATGAAGCGGCAATCCAATATGA GTCTCAGGAAGGACATGCGCGAGTTCTCTATTCTCGAAAAGCAAACTCAGTCACGCGGAAGGGACGCCGG AACTCCCGCGCCTATGCAGGAGGAGAACGGGACAACAGCAATAATGGAAACCCCGAAACAGAAGCTCGAC TTTATCGGGAACTCCACAGGCCACAAGCGACGGCCTCGCACACCCAAAAATAGGGCTCAGCCCCTTGAGG ACCTTGACGGATTTCAGGAGCTGTTTCAGACGCCCGCTGGGGCTTCCGACCCTGTTTCCGTGGAGGAATC AGCGAAGATCTCCCTGGCATCTTCACAGGCCGAGCCAGTCAGGACCCCCGCCAGCACCAAGCGACGCAGC AAGACTGGACTTAGTAAGGTCGATGTTAGACAAGAGCCTAGCACGCTGGGAAAACGCATGAAATCTCTGG GAAGAGCTCCGGGTACGCCAGCACCCGTGCAGGAGGAGAACGACAGCACCGCCTTCATGGAGACTCCAAA ACAGAAGCTGGACTTCACCGGAAACAGTAGCGGTCATAAGCGACGACCACAGACGCCAAAGATTAGGGCA CAGCCTTTGGAAGACCTTGATGGCTTCCAGGAGCTGTTCCAGACGCCAGCGGGTGCTAACGATTCTGTTA CAGTCGAGGAGTCAGTGAAGATGTCACTGGAATCCTCACAAGCTGAGCCTGTGAAAACACCCGCCTCTAC CAAGAGATTGTCAAAAACTGGCTTGTCAAAGGTGGATGTGCGGGAAGATCCCAGCATCCTGGAAAAAAAG ACAAAGTCTCCCGGTACCCCCGCCCCCGTGCAGGAGGAAAATGATTGTACCGCCTTCATGGAGACACCAA AGCAGAAACTCGATTTTACCGGGAATAGCTCAGGTCACAAGCGACGGCCCAGGACCCCCAAGATCAGGGC TCAGCCCCTCGAAGACCTTGATGGGTTCCAGGAGCTGTTCCAGACCCCAGCAGGCGCTTCCGACTCCGTG ACCGTCGAAGAATCAGCAAAGATGTCCCTGGAGAGCAGTCAGGCTAAGCCTGTGAAGACTCCTGCTTCAA CCAAAAGGCTCAGCAAGACAGGGCTGAGCAAAGTCGATGTACGGGAGGACCCCAGCACCCTGGGGAAGAA AACGAAGTCACCCGGACGCGCACCTGGTACACCAGCACCTGTGCAAGAGGAGAACGATAGCACAGCCTTT ATGGAGACACCCAAGCAGAAGCTCGACTTCGCAGAAAACTCATCCGGATCAAAGCGACGGAGCAGAACCA GCAAGAATAGGAGCCAACCACTTGAAGACCTGGACGGGTTTCAGGAGCTGTTCCAGACACCCGCAGGAGC CTCCAATCCCGTATCCGTGGAAGAATCTGCTAAGATCTCACTTGAAAGCAGCCAAGCTGAGCCCGTACGC ACCAGGGCCTCCACAAAGCGACTTTCAAAGACCGGCTTGAACAAGATGGACGTCAGAGAGGGGCACTCTC CCCTGAGCAAATCCTCTTGCGCCAGTCAGAAGGTAATGCAGACTCTCACCCTGGGCGAGGATCATGGACG AGAAACTAAAGACGGAAAGGTCCTTCTTGCGCAGAAGCTGGAGCCAGCTATTTATGTCACGCGAGGCAAA CGACAGCAAAGGAGTTGCAAGAAGCGCAGCCAGTCACCAGAGGATTTGTCCGGTGTGCAGGAGGTTTTTC AGACATCTGGGCACAATAAAGATAGCGTCACTGTGGATAATTTGGCCAAGCTGCCCTCCTCTTCTCCACC TCTTGAACCTACCGATACTAGCGTGACCAGTAGGCGGCAAGCTCGCACCGGACTTCGGAAGGTCCATGTG AAGAACGAACTGAGTGGCGGGATAATGCACCCACAGATCAGCGGGGAGATAGTTGACCTGCCTCGGGAAC CGGAGGGCGAGGGTAAAGTGATTAAGACTCGGAAGCAGTCTGTGAAGCGCAAACTGGATACGGAGGTGAA CGTCCCACGCTCAAAGAGGCAGAGGATAACACGGGCTGAAAAGACGCTTGAAGACCTGCCAGGGTTCCAA GAACTGTGTCAGGCACCCTCTCTGGTAATGGACAGCGTAATCGTTGAAAAGACACCCAAGATGCCTGATA AGTCTCCCGAGCCCGTCGACACAACTTCTGAGACACAGGCGAGACGGAGACTGCGAAGACTTGTTGTCAC GGAAGAGCCTATCCCTCAGAGAAAAACAACACGAGTGGTCCGCCAAACTCGGAACACCCAGAAGGAGCCT ATCAGCGATAACCAAGGCATGGAAGAATTCAAGGAATCCTCCGTGCAGAAGCAGGACCCTTCCGTGAGCC TGACCGGTCGACGCAATCAACCCCGGACGGTCAAAGAAAAAACCCAGCCTCTGGAGGAGCTGACATCCTT CCAGGAGGAAACTGCAAAACGAATAAGTTCTAAAAGCCCCCAGCCCGAGGAGAAGGAGACACTTGCGGGA CTGAAGAGGCAGTTGCGGATTCAGTTGATCAACGACGGCGTGAAAGAGGAGCCTACTGCCCAGAGAAAGC AACCGAGCCGCGAGACTCGAAATACACTGAAAGAGCCTGTTGGCGACTCAATTAACGTGGAAGAGGTAAA GAAAAGCACCAAACAGAAGATCGATCCAGTTGCATCCGTGCCTGTGTCCAAGCGGCCCAGACGGGTGCCC AAGGAGAAGGCCCAGGCACTGGAGTTGGCCGGTCTGAAAGGCCCCATCCAGACCCTGGGACACACCGACG AGTCCGCGTCCGACAAAGGACCTACTCAAATGCCCTGTAACAGCTTGCAGCCCGAGCAGGTTGACAGCTT TCAAAGCAGTCCTAGACGGCCTAGGACAAGAAGGGGGAAAGTGGAAGCTGATGAAGAACCATCTGCTGTG AGAAAAACAGTCTCTACGTCCAGGCAGACCATGAGGAGCAGGAAGGTGCCCGAGATCGGGAATAATGGCA CACAGGTGTCAAAAGCCTCAATCAAGCAGACATTGGATACCGTTGCTAAGGTGACTGGGAGCAGACGACA GCTTAGGACACACAAGGACGGTGTACAACCACTCGAGGTCTTGGGAGACTCCAAGGAAATCACCCAGATT AGCGATCATTCCGAAAAGCTGGCCCACGATACGAGCATCCTGAAATCAACACAGCAGCAAAAGCCCGACT CTGTGAAACCGCTGCGGACCTGTCGGAGAGTGCTGCGGGCCTCCAAGGAAGACCCAAAGGAAGTGCTGGT GGACACACGGGACCACGCCACATTGCAGAGTAAGAGTAATCCCCTGCTCTCTCCCAAGAGGAAGTCCGCT CGCGACGGCTCTATCGTAAGAACCAGAGCACTGAGGTCCCTGGCCCCTAAACAGGAGGCCACTGATGAAA AGCCTGTCCCCGAAAAGAAGAGAGCAGCCTCCAGCAAAAGACATGTCAGTCCAGAGCCAGTGAAGATGAA GCACCTTAAGATCGTGAGCAATAAACTTGAGTCCGTGGAGGAGCAAGTGAGCACAGTTATGAAGACCGAG GAGATGGAGGCTAAACGCGAGAACCCTGTCACACCTGATCAGAACAGTCGGTACCGAAAGAAGACTAACG TGAAGCAGCCTCGGCCGAAGTTCGATGCGTCCGCGGAAAATGTGGGCATAAAGAAAAACGAGAAGACGAT GAAAACGGCTTCCCAGGAAACAGAGCTTCAGAATCCGGATGACGGTGCCAAAAAGTCTACGTCCAGAGGG CAGGTTTCAGGAAAGCGGACTTGTCTCAGATCAAGGGGGACAACCGAGATGCCACAGCCTTGCGAAGCAG AGGAAAAAACCAGTAAGCCTGCCGCCGAAATCCTGATTAAACCCCAGGAAGAGAAGGGAGTCAGTGGAGA ATCAGATGTCAGGTGCTTGAGGTCTAGAAAGACCAGGGTCGCCCTGGACAGCGAGCCAAAGCCCCGAGTG ACTAGAGGTACCAAAAAGGATGCCAAGACGCTGAAAGAGGATGAGGATATCGTCTGCACCAAGAAGCTGC GGACCAGGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227041 representing NM_001081117
Red=Cloning site Green=Tags(s) MASSAHLVTIKRSGDDGAHFPLSLSSCLFGRSIECDIRIQLPVVSKRHCKIEVKEQEAILYNFSSTNPTQ VNGVTIDEPVRLRHGDIITIIDRSFRYEDGNHEDGSKPTEFPGKSLGKEPSRRASRDSFCADPDGEGQDT KASKMTASRRSFVYAKGLSADSPASDGSKNSVSQDSSGHVEQHTGRNIVEPTSGDLFKKSRSTGSSYREP KSSPTQSLSNSNEKESPFEKLYQSMKEELDVKSQKSCRKSEPQPDRAAEESRETQLLVSGRARAKSSGST PVTAASSPKVGKIWTERWRGGMVPVQTSTETAKMKTPVRHSQQLKDEDSRVTGRRHSVNLDEGESAQAVH KTVTPGKLATRNQAAVEAGDVASPADTPEHSSSKKRSIPAKVEAPSAETQKRLSLTQRLVPGEKKTPKGS FSKPEKLATAAEQTCSGLPGLSSVDISNFGDSINKSEGMPMKRRRVSFGGHLRPELFDENLPPNTPLKRG ETPTKRKSLGTHSPAVLKTIIKERPQSPGKQESPGITPPRTNDQRRRSGRTSSGSKFLCETDIPKKAGRK SGNLPAKRASISRSQHGILQMICSKRRSGASEANLIVAKSWADVVKLGVKQTQTKVAKHVPQKQTSKRQR RPSTPKKPTSNLHNQFTTGHANSPCTIVVGRAQIEKVSVPARPYKMLNNLMLNRKVDFSEDLSGLTEMFK TPVKEKQQQMSDTGSVLSNSANLSERQLQVTNSGDIPEPITTEILGEKVLSSTRNAAKQQSDRYSASPTL RRRSIKHENTVQTPKNVHNITDLEKKTPVSETEPLKTASSVSKLRRSRELRHTLVETMNEKTEAVLAENT TARHLRGTFREQKVDQQVQDNENAPQRCKESGELSEGSEKTSARRSSARKQKPTKDLLGSQMVTQTADYA EELLSQGQGTIQNLEESMHMQNTSISEDQGITEKKVNIIVYATKEKHSPKTPGKKAQPLEGPAGLKEHFE TPNPKDKPITEDRTRVLCKSPQVTTENITTNTKPQTSTSGKKVDMKEESSALTKRIHMPGESRHNPKILK LECEDIKALKQSENEMLTSTVNGSKRTLEKSKKKAQPLEDLTCFQELFISPVPTNIIKKIPSKSPHTQPV RTPASTKRLSKTGLSKVDVRQEPSTLGKRTKSPGRAPGTPAPVQEENDSTAFMETPKQKLDFAGNSSGSK RRSRTSKNRSQPLEDLDGFQELFQTPAGASDSVTVEESAKISLESSQAEPVKTPASTKRRSKMSLMKVDM KELSILEKQTQSRGRDAGTPAPMQEGNGTTAIMETPKQKLDFTGNSTGHKRRPRTPKIRAQPLEDLDGFQ ELFQTPAGANDSVTVEESAKMSLESSQAEPVKTPASTKRLSKTDLSKVDVREDPSILGKKTKSPGRAPGT PAPVQEENDCTAYMETPKQKLESIENLTGLRKQSRTPKDITGFQDSFQIPDHANGPLVVVKTKKMFFNSP QPESAITRKSRERQSRASISKIDVKEELLESEEHLQLGEGVDTFQVSTNKVIRSSRKPAKRKLDSTAGMP NSKRMRCSSKDNTPCLEDLNGFQELFQMPGYANDSLTTGISTMLARSPQLGPVRTQINKKSLPKIILRKM DVTEEISGLWKQSLGRVHTTQEQEDNAIKAIMEIPKETLQTAADGTRLTRQPQTPKEKVQPLEDHSVFQE LFQTSRYCSDPLIGNKQTRMSLRSPQPGFVRTPRTSKRLAKTSVGNIAVREKISPVSLPQCATGEVVHIP IGPEDDTENKGVKESTPQTLDSSASRTVSKRQQGAHEERPQFSGDLFHPQELFQTPASGKDPVTVDETTK IALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLD FIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRS KTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRA QPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKK TKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTPAGASDSV TVEESAKMSLESSQAKPVKTPASTKRLSKTGLSKVDVREDPSTLGKKTKSPGRAPGTPAPVQEENDSTAF METPKQKLDFAENSSGSKRRSRTSKNRSQPLEDLDGFQELFQTPAGASNPVSVEESAKISLESSQAEPVR TRASTKRLSKTGLNKMDVREGHSPLSKSSCASQKVMQTLTLGEDHGRETKDGKVLLAQKLEPAIYVTRGK RQQRSCKKRSQSPEDLSGVQEVFQTSGHNKDSVTVDNLAKLPSSSPPLEPTDTSVTSRRQARTGLRKVHV KNELSGGIMHPQISGEIVDLPREPEGEGKVIKTRKQSVKRKLDTEVNVPRSKRQRITRAEKTLEDLPGFQ ELCQAPSLVMDSVIVEKTPKMPDKSPEPVDTTSETQARRRLRRLVVTEEPIPQRKTTRVVRQTRNTQKEP ISDNQGMEEFKESSVQKQDPSVSLTGRRNQPRTVKEKTQPLEELTSFQEETAKRISSKSPQPEEKETLAG LKRQLRIQLINDGVKEEPTAQRKQPSRETRNTLKEPVGDSINVEEVKKSTKQKIDPVASVPVSKRPRRVP KEKAQALELAGLKGPIQTLGHTDESASDKGPTQMPCNSLQPEQVDSFQSSPRRPRTRRGKVEADEEPSAV RKTVSTSRQTMRSRKVPEIGNNGTQVSKASIKQTLDTVAKVTGSRRQLRTHKDGVQPLEVLGDSKEITQI SDHSEKLAHDTSILKSTQQQKPDSVKPLRTCRRVLRASKEDPKEVLVDTRDHATLQSKSNPLLSPKRKSA RDGSIVRTRALRSLAPKQEATDEKPVPEKKRAASSKRHVSPEPVKMKHLKIVSNKLESVEEQVSTVMKTE EMEAKRENPVTPDQNSRYRKKTNVKQPRPKFDASAENVGIKKNEKTMKTASQETELQNPDDGAKKSTSRG QVSGKRTCLRSRGTTEMPQPCEAEEKTSKPAAEILIKPQEEKGVSGESDVRCLRSRKTRVALDSEPKPRV TRGTKKDAKTLKEDEDIVCTKKLRTRS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001081117 |
ORF Size | 9531 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001081117.2, NP_001074586.2 |
RefSeq Size | 10098 bp |
RefSeq ORF | 9534 bp |
Locus ID | 17345 |
UniProt ID | E9PVX6 |
Gene Summary | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.[UniProtKB/Swiss-Prot Function] |
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FAQs |
SDS |