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Lpcat1 (NM_145376) Mouse Tagged ORF Clone
CAT＃: MG226965
- TrueORF®
Lpcat1 (tGFP-tagged) - Mouse lysophosphatidylcholine acyltransferase 1 (Lpcat1), (10ug)
ORF Plasmid: DDK
"NM_145376" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2900035H07Rik; Aytl2; BB137372; BC005662; C87117; LPCAT; rd11 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226965 representing NM_145376
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGCGGGGCCGCGGGCCGCGGGCCGCCCCCTCCTCCAGCTCTGGGGCAGGCGACGCGCGGCGCC TAGCGCCCCCGGGGCGGAACCCCTTCGTGCACGAGCTGCGACTGAGCGCCCTGCAGAAGGCCCAGGTGGC TTTCATGACGTTGACGCTGTTCCCCATCCGGCTCCTGTTCGCTGCTTTCATGATGCTGCTGGCCTGGCCC TTTGCACTCCTGGCTTCCCTGGGACCTCCTGATAAGGAGCCAGAGCAGCCCCTGGCCTTATGGAGGAAGG TCGTGGACTTCCTGCTCAAGGCCATCATGCGCACCATGTGGTTTGCTGGCGGCTTCCACCGTGTAGCTGT GAAGGGGCGGCAGGCCCTGCCTACTGAGGCTGCTATCCTCACCTTGGCTCCACATTCCTCCTACTTTGAT GCCATCCCGGTCACCATGACCATGTCCTCCATTGTGATGAAGGCAGAGAGCAGAGACATCCCAATCTGGG GAACTCTGATAAGATACATCAGGCCAGTGTTCGTGTCCCGCTCTGACCAGGACTCGCGAAGGAAGACAGT GGAGGAGATCAAGCGACGGGCACAGTCGAATGGAAAGTGGCCTCAGATAATGATTTTTCCAGAAGGAACT TGTACAAATAGGACCTGCCTCATTACCTTCAAACCTGGTGCGTTCATTCCTGGAGTTCCTGTCCAGCCCG TGGTGCTACGCTACCCAAACAAATTGGACACCATCACATGGACGTGGCAAGGACCTGGAGCGTTGAAAAT CCTGTGGCTCACTCTGTGCCAGTTTCAAAACCAAGTGGAAATTGAATTTCTGCCTGTGTATTGCCCTTCT GAAGAGGAGAAGAGGAATCCTGCCCTGTATGCCAGCAATGTGAGGCGTGTCATGGCCAAGGCCCTGGGTG TCTCAGTAACCGACTATACATTTGAGGACTGCCAGCTGGCTCTGGCAGAAGGACAACTTCGCTTGCCTGC TGACACCTGCCTGCTAGAGTTTGCCAGGCTTGTGAGGGGCCTCGGACTAAAACCAGAAAATCTTGAAAAA GATCTAGACAAATATTCAGAAAGTGCGAGGATGAAGAGAGGAGAGAAAATCCGCCTTCCAGAGTTTGCGG CCTACTTGGAAGTTCCTGTCTCAGATGCACTGGAAGACATGTTCTCGCTTTTTGACGAGAGTGGTGGTGG TGAGATTGACCTTCGTGAATATGTGGTCGCCTTGTCTGTGGTGTGCAGGCCATCCCAGACCTTAGCCACC ATCCAGCTGGCATTCAAGATGTACGGATCTCCTGAGGATGGCAGCATAGATGAGGCCAACCTGTCCTGCA TCCTCAAGACTGCACTGGGTGTATCAGAATTAACAGTGACCGACTTGTTCCAGGCTATTGACCAAGAGGA CAAGGGAAGAATCACCTTTGATGACTTCTGCGGGTTTGCGGAAATGTACCCCGACTACGCAGAGGACTAC TTGTACCCTGATCAGACACATTTCGACAGCTGTGCACAGACACCCCCAGCACCAACTCCCAATGGCTTCT GCATTGACTTCAGCCCTGAGAACTCAGACTTTGGGAGAAAGAATTCTTGTAAGAAAGCGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226965 representing NM_145376
Red=Cloning site Green=Tags(s) MRLRGRGPRAAPSSSSGAGDARRLAPPGRNPFVHELRLSALQKAQVAFMTLTLFPIRLLFAAFMMLLAWP FALLASLGPPDKEPEQPLALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFD AIPVTMTMSSIVMKAESRDIPIWGTLIRYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGT CTNRTCLITFKPGAFIPGVPVQPVVLRYPNKLDTITWTWQGPGALKILWLTLCQFQNQVEIEFLPVYCPS EEEKRNPALYASNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPENLEK DLDKYSESARMKRGEKIRLPEFAAYLEVPVSDALEDMFSLFDESGGGEIDLREYVVALSVVCRPSQTLAT IQLAFKMYGSPEDGSIDEANLSCILKTALGVSELTVTDLFQAIDQEDKGRITFDDFCGFAEMYPDYAEDY LYPDQTHFDSCAQTPPAPTPNGFCIDFSPENSDFGRKNSCKKAD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145376 |
| ORF Size | 1602 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145376.6 |
| RefSeq Size | 1605 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 210992 |
| UniProt ID | Q3TFD2 |
| Gene Summary | Possesses both acyltransferase and acetyltransferase activities (PubMed:16704971). Activity is calcium-independent (PubMed:18285344). Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC) (PubMed:16704971, PubMed:18156367). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (PubMed:16704971, PubMed:18285344). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (PubMed:16704971). Involved in the regulation of lipid droplet number and size (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217637 | Lpcat1 (untagged) - Mouse lysophosphatidylcholine acyltransferase 1 (Lpcat1), (10ug) |
CNY 6,840.00 |
|
| MR226965 | Lpcat1 (Myc-DDK-tagged) - Mouse lysophosphatidylcholine acyltransferase 1 (Lpcat1) |
CNY 3,976.00 |
|
| MR226965L3 | Lenti ORF clone of Lpcat1 (Myc-DDK-tagged) - Mouse lysophosphatidylcholine acyltransferase 1 (Lpcat1) |
CNY 6,460.00 |
|
| MR226965L4 | Lenti ORF clone of Lpcat1 (mGFP-tagged) - Mouse lysophosphatidylcholine acyltransferase 1 (Lpcat1) |
CNY 6,460.00 |
