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Capn8 (NM_001145806) Mouse Tagged ORF Clone
CAT＃: MG226929
- TrueORF®
Capn8 (tGFP-tagged) - Mouse calpain 8 (Capn8) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001145806" in other vectors (4)
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CNY 4,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | nCL-2; nCL-2' |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226929 representing NM_001145806
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCCTGGCAGCTGGAATATCCAAGCAACGTGCAGCTGCTCAGGGCCTTGGCTCTAACCAGAATG CTGTGAAGTATCTGGGCCAGGACTTCGAGACCCTGAGAAAGCAATGCTTGAACTCAGGGGTCCTATTTAA AGACCCGGAATTCCCGGCATGTCCGTCAGCTTTGGGCTACAGGGATCTTGGACCAGGCTCTGCAGAAACT CAAGGCATCATATGGAAGCGACCCACGGAATTGTGTTCCAACCCTCAGTTTATCGTTGGTGGAGCCACAC GCACAGACATCCGCCAGGGGGGTCTTGGAGACTGTTGGCTTCTGGCAGCCATTGCCTCCCTCACCTTGAA TGAAAAGCTGCTTTATCGAGTGGTTCCTAGAGACCAGAGCTTCCAGAAGAACTATGCTGGCATTTTTCAT TTCCAGTTCTGGCAGTATGGAGAGTGGGTGGAGGTGGTCATTGATGACCGGCTCCCCACCAAGAATGGAC AGCTGCTCTTCCTACACTCCGAAGAAGGCAATGAGTTCTGGAGTGCTCTGCTGGAGAAAGCCTATGCCAA GCTCAATGGTTCGTATGAGGCTCTTGCTGGAGGCTCCACAATTGAGGGGTTTGAGGATTTCACGGGTGGC ATCTCTGAGTTTTATGACTTGAGGAAGCCTCCGGGCAATCTGTACTACACCATCCAGAAGGCCCTGCGCA AAGGCTCTCTGCTGGGCTGCTCCATTGATGTCTCAAACGCAGCCGAAGCAGAAGCCACCACCAGGCAAAA GCTGGTTAAGGGCCACGCATACTCTGTGACTGGAGTCGAAGAGGTGGATTTCCGTGGCCTCCCAGAGAAG CTGATCAGGTTGAGGAACCCGTGGGGTGAAGTGGAGTGGACGGGAGCCTGGAGTGATAGTGCACCTGAGT GGAATTACATAGATCCACAGAAGAAGGGAGAACTGGACAAGAGAGCAGAAGATGGGGAGTTCTGGATGTC CTTTTCGGATTTCTTGAAGCAGTTCTCCCGACTAGAGATATGCAACCTGTCCCCAGACTCTCTGAGCAGT GAGGAGATACACAAATGGAACCTGGTACTCTTCAACGGCCGCTGGACACGGGGTTCTACAGCCGGGGGCT GCCAGAACTACCCAGGCTCCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226929 representing NM_001145806
Red=Cloning site Green=Tags(s) MAALAAGISKQRAAAQGLGSNQNAVKYLGQDFETLRKQCLNSGVLFKDPEFPACPSALGYRDLGPGSAET QGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQSFQKNYAGIFH FQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGG ISEFYDLRKPPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRGLPEK LIRLRNPWGEVEWTGAWSDSAPEWNYIDPQKKGELDKRAEDGEFWMSFSDFLKQFSRLEICNLSPDSLSS EEIHKWNLVLFNGRWTRGSTAGGCQNYPGSF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145806 |
| ORF Size | 1143 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145806.1, NP_001139278.1 |
| RefSeq Size | 2483 bp |
| RefSeq ORF | 1146 bp |
| Locus ID | 170725 |
| UniProt ID | Q91VA3 |
| Gene Summary | Calcium-regulated non-lysosomal thiol-protease (By similarity). Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212327 | Capn8 (untagged) - Mouse calpain 8 (Capn8), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MR226929 | Capn8 (Myc-DDK-tagged) - Mouse calpain 8 (Capn8), transcript variant 2 |
CNY 3,800.00 |
|
| MR226929L3 | Lenti ORF clone of Capn8 (Myc-DDK-tagged) - Mouse calpain 8 (Capn8), transcript variant 2 |
CNY 5,700.00 |
|
| MR226929L4 | Lenti ORF clone of Capn8 (mGFP-tagged) - Mouse calpain 8 (Capn8), transcript variant 2 |
CNY 5,700.00 |
