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Sirpa (NM_007547) Mouse Tagged ORF Clone
CAT＃: MG226909
- TrueORF®
Sirpa (tGFP-tagged) - Mouse signal-regulatory protein alpha (Sirpa) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_007547" in other vectors (5)
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CNY 5,510.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI835480; Bit; CD172a; P84; Ptpns1; SHP-1; SHPS-1; SIRP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226909 representing NM_007547
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCCGGCCCGGCCCCTGGCCGCCTAGGGCCGCTGCTGCTCTGCCTGCTGCTCTCCGCGTCCT GTTTCTGTACAGGAGCCACGGGGAAGGAACTGAAGGTGACTCAGCCTGAGAAATCAGTGTCTGTTGCTGC TGGGGATTCGACCGTTCTGAACTGCACTTTGACCTCCTTGTTGCCGGTGGGACCCATTAGGTGGTACAGA GGAGTAGGGCCAAGCCGGCTGTTGATCTACAGTTTCGCAGGAGAATACGTTCCTCGAATTAGAAATGTTT CAGATACTACTAAGAGAAACAATATGGACTTTTCCATCCGTATCAGTAATGTCACCCCAGCAGATGCTGG CATCTACTACTGTGTGAAGTTCCAGAAAGGATCATCAGAGCCTGACACAGAAATACAATCTGGAGGGGGA ACAGAGGTCTATGTACTCGCCAAACCTTCTCCACCGGAGGTATCCGGCCCAGCAGACAGGGGCATACCTG ACCAGAAAGTGAACTTCACCTGCAAGTCTCATGGCTTCTCTCCCCGGAATATCACCCTGAAGTGGTTCAA AGATGGGCAAGAACTCCACCCCTTGGAGACCACCGTGAACCCTAGTGGAAAGAATGTCTCCTACAACATC TCCAGCACAGTCAGGGTGGTACTAAACTCCATGGATGTTAATTCTAAGGTCATCTGCGAGGTAGCCCACA TCACCTTGGATAGAAGCCCTCTTCGTGGGATTGCTAACCTGTCTAACTTCATCCGAGTTTCACCCACCGT GAAGGTCACCCAACAGTCCCCGACGTCAATGAACCAGGTGAACCTCACCTGCCGGGCTGAGAGGTTCTAC CCCGAGGATCTCCAGCTGATCTGGCTGGAGAATGGAAACGTATCACGGAATGACACGCCCAAGAATCTCA CAAAGAACACGGATGGGACCTATAATTACACAAGCTTGTTCCTGGTGAACTCATCTGCTCATAGAGAGGA CGTGGTGTTCACGTGCCAGGTGAAGCACGACCAACAGCCAGCGATCACCCGAAACCATACCGTGCTGGGA TTTGCCCACTCGAGTGATCAAGGGAGCATGCAAACCTTCCCTGATAATAATGCTACCCACAACTGGAATG TCTTCATCGGTGTGGGCGTGGCGTGTGCTTTGCTCGTAGTCCTGCTGATGGCTGCTCTCTACCTCCTCCG GATCAAACAGAAGAAAGCCAAGGGGTCAACATCTTCCACACGGTTGCACGAGCCCGAGAAGAACGCCAGG GAAATAACCCAGATCCAGGACACAAATGACATCAACGACATCACATACGCAGACCTGAATCTGCCCAAAG AGAAGAAGCCCGCACCCCGGGCCCCTGAGCCTAACAACCACACAGAATATGCAAGCATTGAGACAGGCAA AGTGCCTAGGCCAGAGGATACCCTCACCTATGCTGACCTGGACATGGTCCACCTCAGCCGGGCACAGCCA GCCCCCAAGCCTGAGCCATCTTTCTCAGAGTATGCTAGTGTCCAGGTCCAGAGGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226909 representing NM_007547
Red=Cloning site Green=Tags(s) MEPAGPAPGRLGPLLLCLLLSASCFCTGATGKELKVTQPEKSVSVAAGDSTVLNCTLTSLLPVGPIRWYR GVGPSRLLIYSFAGEYVPRIRNVSDTTKRNNMDFSIRISNVTPADAGIYYCVKFQKGSSEPDTEIQSGGG TEVYVLAKPSPPEVSGPADRGIPDQKVNFTCKSHGFSPRNITLKWFKDGQELHPLETTVNPSGKNVSYNI SSTVRVVLNSMDVNSKVICEVAHITLDRSPLRGIANLSNFIRVSPTVKVTQQSPTSMNQVNLTCRAERFY PEDLQLIWLENGNVSRNDTPKNLTKNTDGTYNYTSLFLVNSSAHREDVVFTCQVKHDQQPAITRNHTVLG FAHSSDQGSMQTFPDNNATHNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAR EITQIQDTNDINDITYADLNLPKEKKPAPRAPEPNNHTEYASIETGKVPRPEDTLTYADLDMVHLSRAQP APKPEPSFSEYASVQVQRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007547 |
| ORF Size | 1527 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007547.4, NP_031573.2 |
| RefSeq ORF | 1530 bp |
| Locus ID | 19261 |
| UniProt ID | P97797 |
| Gene Summary | Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217145 | Sirpa (untagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 1, (10ug) |
CNY 6,560.00 |
|
| MG208194 | Sirpa (tGFP-tagged) - Mouse signal-regulatory protein alpha (Sirpa) |
CNY 7,288.00 |
|
| MR226909 | Sirpa (Myc-DDK-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 1 |
CNY 5,688.00 |
|
| MR226909L3 | Lenti ORF clone of Sirpa (Myc-DDK-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 1 |
CNY 6,940.00 |
|
| MR226909L4 | Lenti ORF clone of Sirpa (mGFP-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 1 |
CNY 8,088.00 |
