Taf9b (NM_001001176) Mouse Tagged ORF Clone
CAT#: MG226816
- TrueORF®
Taf9b (tGFP-tagged) - Mouse TAF9B RNA polymerase II TATA box binding protein (TBP)-associated factor (Taf9b) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001001176" in other vectors (4)
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CNY 5,440.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BC066223; Taf9l |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226816 representing NM_001001176
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGCCAAGATGGCGCCTATCAAGAACGCTCCGAGAGATGCCTTGGTGATGGCACAGATCCTGA AGGATATGGGAATCACAGAGTATGAGCCAAGGGTTATAAATCAAATGTTGGAATTTGCTTTCCGATATGT GACTACAATTCTGGACGATGCAAAGATTTATTCGAGTCATGCTAAGAAACCCACTGTTGATGCCGATGAC GTGAGACTGGCCATCCAGTGTCGGGCTGACCAGTCCTTTACCTCTCCTCCCCCAAGAGATTTTTTACTAG ATATTGCAAGGCAGAAGAATCAAACCCCTTTGCCGCTGATTAAGCCATATGCAGGACCCAGACTGCCACC TGATAGATACTGCTTAACAGCTCCAAACTATAGGCTGAAGTCCTTAGTTAAAAAGGGACCTAACCAAGGA AGACTAGTGCCTAGATTAAGTGCGGTCAGTAGTAGACCAACTACTCCTCCTGTAGCACCCCCACAAGCAG TATCTGGCCCAAATAAGGCTGCAACTCCGGTGTCAGTGACAAGCCAAAGATTTGCTGTGCAGATTCCACC TTCTCAGTCCACACCTGCCAAGCCAGCTCCTGCAGCAACTGCAGTCCAAAATGTTCTGATTAATCCTTCA ATGATTGGGCCCAAAAATATTCTCATTACCACCAGCATGGTTTCCTCACAGAACACAGCCACCGACTCAA ACCCACTGAAGAGGAAGCATGATGACGACGATGACAATGACACCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226816 representing NM_001001176
Red=Cloning site Green=Tags(s) MEPAKMAPIKNAPRDALVMAQILKDMGITEYEPRVINQMLEFAFRYVTTILDDAKIYSSHAKKPTVDADD VRLAIQCRADQSFTSPPPRDFLLDIARQKNQTPLPLIKPYAGPRLPPDRYCLTAPNYRLKSLVKKGPNQG RLVPRLSAVSSRPTTPPVAPPQAVSGPNKAATPVSVTSQRFAVQIPPSQSTPAKPAPAATAVQNVLINPS MIGPKNILITTSMVSSQNTATDSNPLKRKHDDDDDNDTM TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001001176 |
ORF Size | 747 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001001176.2, NP_001001176.2 |
RefSeq Size | 2602 bp |
RefSeq ORF | 750 bp |
Locus ID | 407786 |
UniProt ID | Q6NZA9 |
Gene Summary | Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202724 | Taf9b (untagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf9b), transcript variant 2, (10ug) |
CNY 2,000.00 |
|
MR226816 | Taf9b (Myc-DDK-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf9b), transcript variant 2 |
CNY 2,470.00 |
|
MR226816L3 | Lenti ORF clone of Taf9b (Myc-DDK-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf9b), transcript variant 2 |
CNY 4,370.00 |
|
MR226816L4 | Lenti ORF clone of Taf9b (mGFP-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf9b), transcript variant 2 |
CNY 4,370.00 |