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Ppargc1b (NM_133249) Mouse Tagged ORF Clone
CAT＃: MG226760
- TrueORF®
Ppargc1b (tGFP-tagged) - Mouse peroxisome proliferative activated receptor gamma coactivator 1 beta (Ppargc1b), (10ug)
ORF Plasmid: DDK
"NM_133249" in other vectors (4)
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CNY 9,690.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4631412G21Rik; Perc |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226760 representing NM_133249
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGAACGACTGCGGCGCGCTGCTGGATGAAGAGCTCTCGTCCTTCTTCCTCAACTATCTCTCTG ACACGCAGGGTGGGGACTCTGGAGAGGAACAGCTGTGTGCTGACTTGCCAGAGCTTGACCTCTCCCAGCT GGACGCCAGTGACTTTGACTCAGCCACGTGCTTTGGGGAGCTGCAGTGGTGCCCGGAGACCTCAGAGACA GAGCCCAGCCAGTACAGCCCCGATGACTCCGAGCTCTTCCAGATTGACAGTGAGAATGAAGCTCTCTTGG CTGCGCTTACGAAGACCCTGGATGACATCCCCGAAGACGATGTGGGGCTGGCTGCCTTCCCAGAACTGGA TGAAGGCGACACACCATCCTGCACCCCAGCCTCACCTGCCCCCTTATCTGCACCCCCCAGCCCCACCCTG GAGAGGCTTCTGTCCCCAGCGTCTGACGTGGACGAGCTTTCACTGCTACAGAAGCTCCTCCTGGCCACAT CCTCCCCAACAGCAAGCTCTGACGCTCTGAAGGACGGGGCCACCTGGTCCCAGACCAGCCTCAGTTCCAG AAGTCAGCGGCCTTGTGTCAAGGTGGATGGCACCCAGGATAAGAAGACCCCCACACTGCGGGCTCAGAGC CGGCCTTGTACGGAACTGCATAAGCACCTCACTTCGGTGCTGCCCTGTCCCAGAGTGAAAGCCTGCTCCC CAGCTCCGCACCCGAGCCCTCGGCTCCTCTCCAAAGAGGAGGAGGAGGAGGTGGGGGAGGATTGCCCAAG CCCTTGGCCGACTCCAGCCTCGCCCCAAGACTCCCTAGCACAGGACACGGCCAGCCCCGACAGTGCCCAG GCTCCCGAGGAGGATGTGAGGGCCATGGTACAGCTCATTCGCTACATGCATACCTACTGCCTGCCTCAGA GGAAGCTGCCCCAACGGGCCCCAGAGCCAATCCCCCAGGCCTGCAGCAGCCTCTCCAGGCAGGTTCAACC CCGATCCCGGCATCCCCCCAAAGCCTTCTGGACTGAGTTCTCTATCCTAAGGGAACTTCTGGCCCAAGAT ATCCTCTGTGATGTTAGCAAGCCCTACCGCCTGGCCATACCTGTCTATGCTTCCCTCACACCTCAGTCCA GGCCCAGGCCCCCCAAGGACAGTCAGGCCTCCCCTGCCTACTCTGCCATGGCAGAAGAGGTGAGAATCAC TGCTTCCCCCAAGAGCACCGGGCCTAGACCCAGCCTGCGTCCTCTGAGGCTGGAGGTGAAACGGGATGTT AACAAGCCTACAAGGCAAAAGCGGGAGGAAGATGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAAGAAG AAAAAGAAGAGGAAGAAGAGGAGTGGGGCAGGAAGAGACCAGGTCGTGGCCTGCCATGGACCAAACTAGG GAGGAAGATGGACAGCTCCGTGTGCCCCGTGCGGCGCTCCAGGAGACTGAATCCAGAGCTGGGTCCCTGG CTGACATTCACTGATGAGCCCTTAGGTGCTCTGCCCTCGATGTGCCTGGATACAGAGACCCACAACCTGG AGGAAGACCTGGGCAGCCTCACAGACAGTAGTCAAGGCCGGCAGCTCCCCCAGGGATCCCAGATCCCCGC CCTGGAAAGCCCCTGTGAGAGTGGGTGCGGAGACACAGATGAAGATCCAAGCTGCCCACAGCCCACTTCC AGAGACTCCTCCAGGTGCCTCATGCTGGCCTTGTCACAAAGCGACTCTCTTGGCAAGAAGAGCTTTGAGG AGTCCCTGACGGTGGAGCTTTGCGGCACGGCAGGACTCACGCCACCCACCACACCTCCATACAAGCCAAT GGAGGAGGACCCCTTCAAGCCAGACACCAAGCTCAGCCCAGGCCAAGACACAGCTCCCAGCCTTCCCTCC CCCGAGGCTCTTCCGCTCACAGCCACCCCAGGAGCTTCCCACAAGCTGCCCAAGAGGCACCCAGAGCGAA GCGAGCTCCTGTCCCATTTGCAGCATGCCACAACCCAACCAGTCTCACAGGCTGGCCAGAAGCGCCCCTT CTCCTGCTCCTTTGGAGACCACGACTACTGCCAGGTGCTCAGGCCAGAGGCTGCCCTGCAGAGGAAGGTG CTGCGGTCCTGGGAGCCAATCGGGGTCCACCTTGAAGACTTGGCCCAGCAGGGTGCCCCTCTGCCAACGG AAACAAAGGCCCCTAGGAGGGAGGCAAACCAGAACTGTGACCCTACCCACAAGGACAGCATGCAGCTAAG AGACCATGAGATCCGTGCCAGTCTCACAAAGCACTTTGGGCTGCTGGAGACTGCTCTGGAAGGTGAAGAC CTGGCGTCCTGTAAAAGCCCGGAGTATGACACCGTATTTGAGGACAGCAGCAGCAGCAGTGGCGAGAGTA GCTTCCTGCTTGAGGAGGAGGAGGAAGAGGAGGAGGGAGGGGAAGAGGACGATGAAGGAGAGGACTCAGG GGTCAGCCCTCCCTGCTCTGATCACTGCCCCTACCAGAGCCCACCCAGTAAGGCCAGTCGGCAGCTCTGC TCCCGAAGCCGCTCCAGTTCCGGCTCCTCGTCCTGCAGCTCCTGGTCACCAGCCACCCGGAAGAACTTCA GACGTGAGAGCAGAGGGCCCTGTTCAGATGGAACCCCAAGCGTCCGGCATGCCAGGAAGCGGCGGGAAAA GGCCATCGGTGAAGGCCGTGTGGTATACATTCGAAATCTCTCCAGTGACATGAGCTCTCGGGAACTAAAG AAGCGCTTTGAGGTGTTCGGTGAGATTGTAGAGTGCCAGGTGCTGACGAGAAGTAAAAGAGGCCAGAAGC ACGGTTTTATCACCTTCCGGTGTTCAGAGCACGCTGCCCTGTCCGTGAGGAACGGCGCCACCCTGAGAAA GCGCAATGAGCCCTCCTTCCACCTGAGCTATGGAGGGCTCCGGCACTTCCGTTGGCCCAGATACACTGAC TATGATCCCACATCTGAGGAGTCCCTTCCCTCATCTGGGAAAAGCAAGTACGAAGCCATGGATTTTGACA GCTTACTGAAAGAGGCCCAGCAGAGCCTGCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226760 representing NM_133249
Red=Cloning site Green=Tags(s) MAGNDCGALLDEELSSFFLNYLSDTQGGDSGEEQLCADLPELDLSQLDASDFDSATCFGELQWCPETSET EPSQYSPDDSELFQIDSENEALLAALTKTLDDIPEDDVGLAAFPELDEGDTPSCTPASPAPLSAPPSPTL ERLLSPASDVDELSLLQKLLLATSSPTASSDALKDGATWSQTSLSSRSQRPCVKVDGTQDKKTPTLRAQS RPCTELHKHLTSVLPCPRVKACSPAPHPSPRLLSKEEEEEVGEDCPSPWPTPASPQDSLAQDTASPDSAQ APEEDVRAMVQLIRYMHTYCLPQRKLPQRAPEPIPQACSSLSRQVQPRSRHPPKAFWTEFSILRELLAQD ILCDVSKPYRLAIPVYASLTPQSRPRPPKDSQASPAYSAMAEEVRITASPKSTGPRPSLRPLRLEVKRDV NKPTRQKREEDEEEEEEEEEEEEEKEEEEEEWGRKRPGRGLPWTKLGRKMDSSVCPVRRSRRLNPELGPW LTFTDEPLGALPSMCLDTETHNLEEDLGSLTDSSQGRQLPQGSQIPALESPCESGCGDTDEDPSCPQPTS RDSSRCLMLALSQSDSLGKKSFEESLTVELCGTAGLTPPTTPPYKPMEEDPFKPDTKLSPGQDTAPSLPS PEALPLTATPGASHKLPKRHPERSELLSHLQHATTQPVSQAGQKRPFSCSFGDHDYCQVLRPEAALQRKV LRSWEPIGVHLEDLAQQGAPLPTETKAPRREANQNCDPTHKDSMQLRDHEIRASLTKHFGLLETALEGED LASCKSPEYDTVFEDSSSSSGESSFLLEEEEEEEEGGEEDDEGEDSGVSPPCSDHCPYQSPPSKASRQLC SRSRSSSGSSSCSSWSPATRKNFRRESRGPCSDGTPSVRHARKRREKAIGEGRVVYIRNLSSDMSSRELK KRFEVFGEIVECQVLTRSKRGQKHGFITFRCSEHAALSVRNGATLRKRNEPSFHLSYGGLRHFRWPRYTD YDPTSEESLPSSGKSKYEAMDFDSLLKEAQQSLH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133249 |
| ORF Size | 3042 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133249.2, NP_573512.1 |
| RefSeq Size | 3656 bp |
| RefSeq ORF | 3045 bp |
| Locus ID | 170826 |
| UniProt ID | Q8VHJ7 |
| Gene Summary | Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be part of the pathways regulating the elevation of gluconeogenesis, beta-oxidation of fatty acids and ketogenesis during fasting. Stimulates SREBP-mediated lipogenic gene expression in the liver. Induces energy expenditure and antagonizes obesity when overexpressed. Induces also the expression of mitochondrial genes involved in oxidative metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223214 | Ppargc1b (untagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b), (10ug) |
CNY 13,110.00 |
|
| MR226760 | Ppargc1b (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b) |
CNY 7,072.00 |
|
| MR226760L3 | Lenti ORF clone of Ppargc1b (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b) |
CNY 10,740.00 |
|
| MR226760L4 | Lenti ORF clone of Ppargc1b (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b) |
CNY 10,740.00 |
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