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Cbln1 (NM_019626) Mouse Tagged ORF Clone
CAT＃: MG226558
- TrueORF®
Cbln1 (tGFP-tagged) - Mouse cerebellin 1 precursor protein (Cbln1), (10ug)
ORF Plasmid: DDK
"NM_019626" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI323299 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226558 representing NM_019626
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGCGTCGTGGAGCTGCTGCTGTTGGGGACTGCGTGGCTGGCAGGCCCAGCCCGCGGGCAGAATG AGACAGAGCCCATCGTACTGGAGGGCAAGTGCCTGGTGGTGTGTGACTCCAACCCCACGTCTGACCCTAC GGGCACTGCTCTGGGCATCTCTGTGCGCTCCGGCAGCGCCAAGGTGGCTTTCTCTGCCATCAGGAGCACC AACCATGAGCCGTCCGAGATGAGTAATCGCACCATGATCATCTACTTCGACCAGGTACTAGTGAACATCG GGAACAACTTTGACTCAGAACGCAGCACTTTCATCGCCCCGCGCAAAGGCATCTACAGTTTTAACTTCCA CGTGGTGAAAGTCTACAACAGACAGACCATCCAGGTGAGCCTCATGTTGAACGGGTGGCCGGTGATTTCA GCCTTCGCCGGTGACCAAGACGTGACACGCGAGGCCGCCAGCAACGGCGTCCTCATCCAGATGGAGAAAG GCGACCGAGCATACCTCAAGCTGGAGCGGGGGAACTTGATGGGGGGCTGGAAGTACTCAACCTTCTCTGG ATTCCTCGTGTTTCCCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226558 representing NM_019626
Red=Cloning site Green=Tags(s) MLGVVELLLLGTAWLAGPARGQNETEPIVLEGKCLVVCDSNPTSDPTGTALGISVRSGSAKVAFSAIRST NHEPSEMSNRTMIIYFDQVLVNIGNNFDSERSTFIAPRKGIYSFNFHVVKVYNRQTIQVSLMLNGWPVIS AFAGDQDVTREAASNGVLIQMEKGDRAYLKLERGNLMGGWKYSTFSGFLVFPL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019626 |
| ORF Size | 579 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019626.3, NP_062600.2 |
| RefSeq Size | 2345 bp |
| RefSeq ORF | 582 bp |
| Locus ID | 12404 |
| UniProt ID | Q9R171 |
| Gene Summary | Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the matching and maintenance of pre- and post-synaptic elements at parallel fiber-Purkinje cell synapses, the establishment of the proper pattern of climbing fiber-Purkinje cell innervation, and induction of long-term depression at parallel fiber-Purkinje cell synapses (PubMed:16234806). Plays a role as a synaptic organizer that acts bidirectionally on both pre- and post-synaptic components (PubMed:20395510). On the one hand induces accumulation of synaptic vesicles in the pre-synaptic part by binding with NRXN1 and in other hand induces clustering of GRID2 and its associated proteins at the post-synaptic site through association of GRID2 (PubMed:21410790). NRXN1-CBLN1-GRID2 complex directly induces parallel fiber protrusions that encapsulate spines of Purkinje cells leading to accumulation of GRID2 and synaptic vesicles (PubMed:23141067). Required for CBLN3 export from the endoplasmic reticulum and secretion (PubMed:17030622, PubMed:17331201). NRXN1-CBLN1-GRID2 complex mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208223 | Cbln1 (untagged) - Mouse cerebellin 1 precursor protein (Cbln1), (10ug) |
CNY 3,990.00 |
|
| MR226558 | Cbln1 (Myc-DDK-tagged) - Mouse cerebellin 1 precursor protein (Cbln1) |
CNY 2,400.00 |
|
| MR226558L3 | Lenti ORF clone of Cbln1 (Myc-DDK-tagged) - Mouse cerebellin 1 precursor protein (Cbln1) |
CNY 4,750.00 |
|
| MR226558L4 | Lenti ORF clone of Cbln1 (mGFP-tagged) - Mouse cerebellin 1 precursor protein (Cbln1) |
CNY 4,750.00 |
