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Usp46 (NM_177561) Mouse Tagged ORF Clone
CAT＃: MG226508
- TrueORF®
Usp46 (tGFP-tagged) - Mouse ubiquitin specific peptidase 46 (Usp46), (10ug)
ORF Plasmid: DDK
"NM_177561" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1190009E20Rik; 2410018I08Rik; AI451644 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226508 representing NM_177561
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGTCCGAAACATCGCCTCCATCTGTAATATGGGCACCAATGCCTCTGCTCTGGAAAAAGACATTG GACCAGAGCAGTTTCCGATCAATGAACACTACTTCGGATTGGTCAATTTTGGAAACACATGCTACTGTAA CTCCGTGCTTCAAGCGCTGTACTTCTGCCGGCCGTTCCGGGAGAATGTGTTGGCATACAAGGCCCAGCAA AAAAAGAAGGAAAACCTGTTGACATGCCTGGCAGACCTTTTCCACAGCATTGCCACGCAGAAGAAGAAGG TTGGCGTCATCCCACCAAAGAAGTTCATTTCAAGGCTGAGGAAAGAGAACGATCTCTTTGATAACTACAT GCAGCAGGATGCTCACGAGTTCTTAAATTATTTGCTAAACACTATTGCGGACATCCTGCAGGAAGAGAAG AAACAGGAAAAACAAAACGGGAAGTTAAAAAACGGCAACATGAACGAACCAGCCGAGAACAATAAGCCAG AACTCACCTGGGTCCACGAGATTTTTCAGGGAACACTCACTAACGAAACTCGATGCTTGAACTGTGAAAC TGTGAGTAGCAAAGATGAAGATTTTCTTGACCTCTCTGTCGATGTGGAACAGAACACCTCCATCACCCAC TGTCTGAGAGACTTCAGCAACACAGAAACGCTGTGTAGTGAACAAAAATATTATTGTGAAACATGTTGCA GCAAACAGGAGGCTCAGAAGAGGATGAGGGTAAAAAAGCTGCCCATGATTTTGGCCCTGCACCTCAAGAG GTTCAAGTACATGGAACAGCTCCACAGGTACACCAAGCTGTCTTACCGGGTGGTCTTCCCTCTGGAACTC CGGCTCTTCAACACCTCCAGCGATGCTGTGAACCTGGACCGCATGTATGACCTGGTTGCTGTGGTCGTTC ACTGTGGAAGTGGTCCTAACCGTGGGCATTACATCACCATCGTAAAAAGTCATGGCTTCTGGCTGTTGTT TGATGACGACATTGTAGAGAAAATAGACGCTCAAGCCATTGAGGAATTCTATGGTCTAACGTCAGATATA TCAAAAAATTCAGAATCTGGATATATTTTATTCTATCAATCAAGAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226508 representing NM_177561
Red=Cloning site Green=Tags(s) MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQ KKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEK KQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITH CLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLEL RLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDI SKNSESGYILFYQSRE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177561 |
| ORF Size | 1098 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177561.3, NP_808229.1 |
| RefSeq Size | 3225 bp |
| RefSeq ORF | 1101 bp |
| Locus ID | 69727 |
| UniProt ID | P62069 |
| Gene Summary | Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67 (PubMed:19465912). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210996 | Usp46 (untagged) - Mouse ubiquitin specific peptidase 46 (Usp46), (10ug) |
CNY 3,990.00 |
|
| MR226508 | Usp46 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 46 (Usp46) |
CNY 3,656.00 |
|
| MR226508L3 | Lenti ORF clone of Usp46 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 46 (Usp46) |
CNY 4,750.00 |
|
| MR226508L4 | Lenti ORF clone of Usp46 (mGFP-tagged) - Mouse ubiquitin specific peptidase 46 (Usp46) |
CNY 4,750.00 |
