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Dhx9 (NM_007842) Mouse Tagged ORF Clone
CAT＃: MG226503
- TrueORF®
Dhx9 (tGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 9 (Dhx9), (10ug)
ORF Plasmid: DDK
"NM_007842" in other vectors (4)
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CNY 15,200.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI326842; Ddx9; HEL-5; mHEL-5; NDHII; RHA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226503 representing NM_007842
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGACATTAAAAATTTTCTGTATGCCTGGTGTGGCAAAAGGAAGATGACTCCAGCCTATGAAATTA GAGCAGTAGGAAACAAAAACAGACAGAAATTCATGTGTGAGGTTCGAGTAGAAGGGTTTAATTATGCTGG TATGGGCAATTCCACCAATAAAAAGGATGCACAGAGCAATGCTGCCAGAGACTTTGTTAACTATTTGGTT CGAATAAATGAAGTAAAGAGTGAAGAAGTTCCAGCTGTTGGGATTGTACCACCCCCACCCATACTTAGCG ACACCTCTGATAGCACAGCAAGTGCTGCAGAAGGGTTACCAGCACCAATGGGAGGACCACTTCCTCCTCA TCTGGCTCTCAAAGCAGAAGAAAATAATTCTGGAGTTGAGTCTTCTGGCTATGGCTCTCCTGGACCTACT TGGGACCGAGGAGCCAACCTTAAAGATTACTACTCAAGAAAGGAAGAACAAGAAGTACAAGCGACTCTAG AGTCAGAAGAAGTGGATTTAAATGCTGGGCTTCATGGAAATTGGACACTGGAAAATGCTAAGGCTCGTCT GAACCAGTATTTCCAAAAAGAAAAGATCCAAGGAGAATATAAGTACACCCAAGTGGGTCCCGACCACAAC AGGAGCTTTATTGCAGAAATGACCATTTATATCAAGCAGCTTGGCAGAAGGATTTTCGCACGTGAACATG GATCTAATAAGAAATTAGCAGCACAGTCATGTGCCCTCTCACTTGTCCGACAGCTCTATCATCTTGGAGT GATTGAGGCTTACTCTGGGCTTACAAAGAAGAAAGAAGGAGAGAGAGTGGAGCCTTACAAAGTTTTCCTT TCTCCAGATTTGGAGCTTCAGCTGCAAAATGTGGTTCAAGAGCTAGATCTTGAAATTGTGCCCCCACCTG TTGATCCTTCTATGCCAGTTATACTTAACATTGGGAAATTGGCTCATTTTGAACCATCTCAGAGACAAAA TGCAGTGGGAGTGGTTCCTTGGTCACCTCCACAATCCAACTGGAATCCTTGGACAAGTAGCAACATTGAT GAGGGACCCCTTGCTTATGCTTCTACAGAACAAATAAGCATGGACCTTAAGAATGAATTAACATACCAGA TGGAACAAGATCATAATTTACAAAGTGTGTTACAAGAGAGAGAGTTGCTGCCTGTGAAGAAATTTGAAGC TGAAATTCTGGAAGCCATTAGTTCAAATTCAGTTGTGATTATCCGAGGGGCTACTGGTTGTGGTAAAACC ACACAAGTTCCACAGTACATTTTGGATGACTTTATCCAGAATGACAGAGCAGCCGAATGCAATATTGTTG TGACACAGCCCAGAAGGATCAGTGCAGTTGCAGTGGCAGAGCGAGTTGCTTATGAGAGAGGAGAAGAGCC TGGAAAAAGCTGTGGTTACAGTGTTCGATTTGAGTCTATACTTCCACGCCCTCATGCCAGTATAATGTTT TGTACTGTAGGTGTGCTTCTAAGAAAACTAGAAGCCGGCATTCGAGGAATCAGTCATGTTATTGTGGATG AAATTCATGAAAGAGATATTAATACTGACTTCCTTTTGGTAGTACTGCGTGATGTGGTTTTGGCTTATCC TGAAGTCCGTATTGTTCTTATGTCTGCAACTATTGATACCACCATGTTCTGTGAATATTTCTTCAATTGC CCCATCATTGAGGTTTATGGAAGGACTTTCCCAGTTCAAGAGTATTTTCTGGAAGACTGCATTCAGATGA CTCAATTCATTCCTCCTCCAAAGGACAAGAAAAAGAAGGATAAGGAGGATGATGGTGGTGAAGATGATGA TGCAAATTGCAACCTAATATGTGGTGACGAATATGGGCCAGAAACAAAGTTGAGCATGTCTCAGTTGAAT GAAAAGGAAACTCCTTTTGAACTTATTGAGGCGCTTCTTAAATACATTGAAACCCTTAATGTTCCTGGAG CTGTGTTGGTATTTTTACCTGGCTGGAACTTGATTTATACTATGCAAAAGCATTTAGAAAATAATTCGCA TTTCGGAAGTCACCGGTATCAGATTCTACCTCTCCATTCTCAGATTCCTCGTGAGGAACAGCGCAAAGTG TTTGATCCAGTACCAGATGGAGTAACCAAGGTTATTTTGTCCACAAACATTGCTGAAACAAGCATTACCA TAAATGATGTTGTTTACGTCATTGACTCCTGCAAACAAAAAGTGAAACTATTTACTGCTCACAACAATAT GACCAACTATGCTACTGTATGGGCATCAAAAACAAACCTTGAGCAGAGGAAAGGGCGAGCTGGCCGTGTA CGACCTGGGTTCTGTTTCCACCTTTGTAGTCGAGCTCGTTTTGACAGACTAGAGACCCACATGACACCAG AGATGTTTAGAACACCATTGCATGAAATTGCTCTGAGCATAAAACTTCTGCGTCTAGGAGGAATTGGCCA ATTCCTTGCAAAGGCAATCGAACCTCCACCTTTGGATGCTGTAATTGAAGCAGAGCATACTCTAAGAGAA CTTGATGCTTTAGATGCCAATGATGAGTTGACTCCTCTTGGACGAATCCTGGCTAAACTCCCCATTGAAC CTCGTTTTGGAAAAATGATGATAATGGGGTGTATTTTCTATGTGGGAGATGCTGTCTGTACCATCTCTGC TGCTACCTGCTTCCCAGAACCCTTTATCAGCGAAGGAAAACGCCTGGGATACATACACCGGAATTTTGCT GGAAACAGATTTTCTGATCACGTGGCTCTTTTATCAGTATTCCAAGCCTGGGATGATGCTAGGATGAGTG GAGAAGAAGCAGAAATACGTTTTTGTGAGCAGAAGAGACTCAATATGGCTACACTGAGAATGACTTGGGA GGCCAAAGTACAGCTTAAAGAGATTCTGATTAACTCTGGATTTCCCGAAGATTGTTTATTGACACAAGTG TTTACTAACACTGGACCAGATAATAATTTGGATGTTGTTATCTCTCTGCTGGCTTTTGGTGTGTACCCCA ATGTATGCTATCATAAGGAGAAGAGAAAGATTCTTACCACTGAAGGGCGCAATGCACTTATCCACAAGTC ATCTGTGAACTGTCCTTTCAGCAGCCAAGACATGAAGTACCCATCTCCTTTCTTTGTTTTTGGTGAAAAG ATTAGAACCCGAGCCATCTCTGCTAAAGGCATGACCTTAGTGACCCCCTTACAGCTACTTCTCTTTGCCT CCAAGAAAGTCCAGTCGGATGGGCAGATTGTGTTTATAGATGACTGGATCAGACTGCAAATATCTCATGA AGCTGCAGCATGCATCACAGCCCTCCGGGCAGCCATGGAAGCTCTGGTTGTTGAAGTGTCCAAACAACCA AATATTATCAGCCAATTGGACCCTGTAAATGAACATATGCTAAACACAATCCGACAGATTTCTAGGCCCT CAGCTGCTGGTATCAACCTTATGATTGGAAGTGTAAGGTACGGCGATGGTCCACGACCTCCCAAGATGGC CCGATATGATAATGGAAGTGGATATAGAAGGGGATATGGTGGGGGAGGATATGGTGGCGGAGGATATGGT GGCGGATACGGTAGTGGAGGCTTTGGAGGAGGCTTTGGAAGTGGGGGAGGCTTTGGAGGAGGCTTTAACA GCGGTGGGGGAGGCTTTGGTAGTGGTGGGGGAGGCTTTGGCAGTGGTGGAGGTGGATTTGGTGGTGGTGG AGGAGGCTTCAGTGGTGGAGGAGGTGGTGGATTTGGTGGTGGCAGAGGAGGTGGAGGTGGCGGATTTGGG GGTAGTGGGGGCTTTGGAAGTGGTGGGGGAGGGTATGGTGTTGGAGGTGGTGGCTATGGAGGAGGTGGTG GTGGTGGCTATGGAGGAGGCAGTGGAGGATATGGAGGTGGGGGAGGGGGTTATGGTGGTGGGGAAGGTTA TAGTATAAGCCCCAACTCTTACCGTGGGAATTATGGTGGTGGCGGGGGAGGGTATAGAGGAGGTTCCCAA GGAGGCTACAGAAACAACTTTGGTGGAGACTACAGAGGGTCCAGTGGAGACTACCGGGGATCCGGTGGGG GCTATAGAGGATCTGGAGGATTCCAGCGAAGGGGTTATGGAGGTGGTTATTTTGGACAAGGAAGAGGAGG AGGGGGTGGTGGTGGCTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226503 representing NM_007842
Red=Cloning site Green=Tags(s) MGDIKNFLYAWCGKRKMTPAYEIRAVGNKNRQKFMCEVRVEGFNYAGMGNSTNKKDAQSNAARDFVNYLV RINEVKSEEVPAVGIVPPPPILSDTSDSTASAAEGLPAPMGGPLPPHLALKAEENNSGVESSGYGSPGPT WDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHN RSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVIEAYSGLTKKKEGERVEPYKVFL SPDLELQLQNVVQELDLEIVPPPVDPSMPVILNIGKLAHFEPSQRQNAVGVVPWSPPQSNWNPWTSSNID EGPLAYASTEQISMDLKNELTYQMEQDHNLQSVLQERELLPVKKFEAEILEAISSNSVVIIRGATGCGKT TQVPQYILDDFIQNDRAAECNIVVTQPRRISAVAVAERVAYERGEEPGKSCGYSVRFESILPRPHASIMF CTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVLAYPEVRIVLMSATIDTTMFCEYFFNC PIIEVYGRTFPVQEYFLEDCIQMTQFIPPPKDKKKKDKEDDGGEDDDANCNLICGDEYGPETKLSMSQLN EKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLENNSHFGSHRYQILPLHSQIPREEQRKV FDPVPDGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRV RPGFCFHLCSRARFDRLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLRE LDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAVCTISAATCFPEPFISEGKRLGYIHRNFA GNRFSDHVALLSVFQAWDDARMSGEEAEIRFCEQKRLNMATLRMTWEAKVQLKEILINSGFPEDCLLTQV FTNTGPDNNLDVVISLLAFGVYPNVCYHKEKRKILTTEGRNALIHKSSVNCPFSSQDMKYPSPFFVFGEK IRTRAISAKGMTLVTPLQLLLFASKKVQSDGQIVFIDDWIRLQISHEAAACITALRAAMEALVVEVSKQP NIISQLDPVNEHMLNTIRQISRPSAAGINLMIGSVRYGDGPRPPKMARYDNGSGYRRGYGGGGYGGGGYG GGYGSGGFGGGFGSGGGFGGGFNSGGGGFGSGGGGFGSGGGGFGGGGGGFSGGGGGGFGGGRGGGGGGFG GSGGFGSGGGGYGVGGGGYGGGGGGGYGGGSGGYGGGGGGYGGGEGYSISPNSYRGNYGGGGGGYRGGSQ GGYRNNFGGDYRGSSGDYRGSGGGYRGSGGFQRRGYGGGYFGQGRGGGGGGGY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007842 |
| ORF Size | 4149 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007842.2, NP_031868.2 |
| RefSeq Size | 4623 bp |
| RefSeq ORF | 4152 bp |
| Locus ID | 13211 |
| UniProt ID | O70133 |
| Gene Summary | Multifunctional ATP-dependent nucleic acid helicase that unwinds DNA and RNA in a 3' to 5' direction and that plays important roles in many processes, such as DNA replication, transcriptional activation, post-transcriptional RNA regulation, mRNA translation and RNA-mediated gene silencing. Requires a 3'-single-stranded tail as entry site for acid nuclei unwinding activities as well as the binding and hydrolyzing of any of the four ribo- or deoxyribo-nucleotide triphosphates (NTPs). Unwinds numerous nucleic acid substrates such as double-stranded (ds) DNA and RNA, DNA:RNA hybrids, DNA and RNA forks composed of either partially complementary DNA duplexes or DNA:RNA hybrids, respectively, and also DNA and RNA displacement loops (D- and R-loops), triplex-helical DNA (H-DNA) structure and DNA- and RNA-based G-quadruplexes. Binds dsDNA, single-stranded DNA (ssDNA), dsRNA, ssRNA and poly(A)-containing RNA. Binds also to circular dsDNA or dsRNA of either linear and/or circular forms and stimulates the relaxation of supercoiled DNAs catalyzed by topoisomerase TOP2A. Plays a role in DNA replication at origins of replication and cell cycle progression. Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors, such as BRCA1, CREBBP, RELA and SMN1. Binds to the CDKN2A promoter. Plays several roles in post-transcriptional regulation of gene expression. In cooperation with NUP98, promotes pre-mRNA alternative splicing activities of a subset of genes (By similarity). As component of a large PER complex, is involved in the negative regulation of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms (PubMed:22767893). Acts also as a nuclear resolvase that is able to bind and neutralize harmful massive secondary double-stranded RNA structures formed by inverted-repeat Alu retrotransposon elements that are inserted and transcribed as parts of genes during the process of gene transposition (PubMed:28355180). Involved in the positive regulation of nuclear export of constitutive transport element (CTE)-containing unspliced mRNA. Component of the coding region determinant (CRD)-mediated complex that promotes cytoplasmic MYC mRNA stability. Plays a role in mRNA translation. Positively regulates translation of selected mRNAs through its binding to post-transcriptional control element (PCE) in the 5'-untranslated region (UTR). Involved with LARP6 in the translation stimulation of type I collagen mRNAs for CO1A1 and CO1A2 through binding of a specific stem-loop structure in their 5'-UTRs. Stimulates LIN28A-dependent mRNA translation probably by facilitating ribonucleoprotein remodeling during the process of translation. Plays also a role as a small interfering (siRNA)-loading factor involved in the RNA-induced silencing complex (RISC) loading complex (RLC) assembly, and hence functions in the RISC-mediated gene silencing process. Binds preferentially to short double-stranded RNA, such as those produced during rotavirus intestinal infection (PubMed:28636595). This interaction may mediate NLRP9 inflammasome activation and trigger inflammatory response, including IL18 release and pyroptosis (PubMed:28636595). Finally, mediates the attachment of heterogeneous nuclear ribonucleoproteins (hnRNPs) to actin filaments in the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224368 | Dhx9 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 9 (Dhx9), (10ug) |
CNY 17,670.00 |
|
| MR226503 | Dhx9 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 9 (Dhx9) |
CNY 14,192.00 |
|
| MR226503L3 | Lenti ORF clone of Dhx9 (Myc-DDK-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 9 (Dhx9) |
CNY 15,680.00 |
|
| MR226503L4 | Lenti ORF clone of Dhx9 (mGFP-tagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 9 (Dhx9) |
CNY 16,592.00 |
