Usp4 (NM_011678) Mouse Tagged ORF Clone
CAT#: MG226489
- TrueORF®
Usp4 (tGFP-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), (10ug)
ORF Plasmid: DDK
"NM_011678" in other vectors (4)
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CNY 9,310.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | F730026I20Rik; mKIAA4155; Unp |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226489 representing NM_011678
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGGCCGGGGCAGCCGTGAGCGACCGGATGTGGAGACCCAGAAGACGGAGCTCGGAGCCTTGA TGGGGACCACGCTCCAACGTGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAA GTATGTGGGCTTTGACAGCTGGGACATGTACAATGTCGGGGAGCATAACCTGTTTCCTGGACCTATTGAC AACTCCGGACTCTTCTCAGATCCTGAGAGTCAGACCTTGAAGGAGCACTTAATCGATGAGCTGGACTATG TGCTGGTCCCAGCCGAAGCCTGGAATAAATTGCTGAATTGGTATGGCTGTGTGGAGGGCCAGCAGCCTAT TGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTGGAAGTGTATTTGCTGGAGCTG AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTAAGTTGCCATTTTAGCAAAGCAGACACCATTGCAA CTATTGAGAAGGAGATGAGGAAGCTCTTCAACATCCCTGCAGAACGTGAAACACGGCTTTGGAACAAATA CATGAGCAACACCTATGAGCAGTTGAGCAAGCTAGACAACACTATCCAGGATGCTGGGCTGTACCAGGGT CAGGTGCTAGTAATTGAGCCCCAAAATGAAGATGGCACATGGCCCCGGCAGAGCCTGCAGTCAAAATCAA GCACTGCACCTAGCAGAAATTTCACTACCTCTTCAAAACCATCCGCAAGTCCCTATTGCTCAGTGTCTGC CTCTCTCATTGCAAATGGTGATAGCACTAACAGCTCTGGGATGCACAGCTCCGGTGTCAGCAGGGGTGGA TCTGGCTTCTCTGCTTCGTATAATTGCCAGGAGCCCCCATCACCTCATATACAGCCTGGCCTCTGTGGAC TTGGAAACCTGGGGAACACTTGCTTCATGAACTCTGCTCTGCAGTGCCTGAGCAACACTGCCCCACTGAC TGAGTACTTTCTCAAAGATGAGTATGAGGCCGAGATCAACCGAGACAACCCTCTGGGGATGAAAGGGGAG ATTGCAGAGGCCTATGCAGAGCTCATCAAGCAGATGTGGTCTGGAAGGGACACTCACGTGGCACCACGGA TGTTCAAGACGCAAGTGGGACGTTTTGCCCCTCAGTTTTCTGGCTACCAACAGCAAGACTCCCAGGAGCT GCTAGCCTTTATTCTAGACGGACTGCACGAGGACCTGAACCGCGTAAAGAAGAAGCCTTACCTGGAGCCC AAGGACGCCAATGGGCGACCAGATGCGGTGGTAGCAAAGGAAGCCTGGGAAAACCACAGGCTGAGGAATG ATTCTGTGATTGTGGATACTTTCCATGGCCTTTTCAAATCGACTTTGGTTTGCCCAGAATGTGCTAAAGT TTCTGTGACCTTTGACCCATTTTGCTATCTAACTCTCCCACTGCCTTTGAAGAAGGATCGGATTATGGAG GTCTTCCTGGTTCCTGCTGACCCTCAGTGCAGACCTATCCAGTACCGTGTGACTGTGCCATTGATGGGGG CCATTTCTGACCTGTGTGAAGCACTCTCCAAGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCACTGA TGTATATAATCACCGCTTCCACAAAATTTTCCAAATGGATGAAGGTTTAAGCCACATCACGCCTCGAGAT GACATTTTTGTGTATGAGGTCTGCAACACGTCCATGGATGGCTCAGAGTGTATCACTCTTCCAGTCTACT TCAGAGAGAAGAAGTCCAGGCCGTCGAGTGCTTCCTCGGGGGCTGTGCTCTATGGACAGCCCCTTCTTGT CTCTGTCCCTAAGCATAAGCTAACCCTAGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTAC ATAAAACAGCCTTTGCCTGATGAGTTTCTCAGCTCACCCTTAGAGCCTGGGGCCTGCAATGGCTCTAGGA GTAGCTATGAAGGAGATGAAGAGGAAGAAATGGATCATCAAGAGGAAGGAAAAGAGCAGCTTTCCGAAGT GGAAGGCAGTGGTGAGGACGATCAGGGAGATGACCATAGCGAGAGCGCCCAAAAGGTGAAAGGCCAGCCG AGGCACAAGAGGCTCTTTACCTTCAGCCTCGTGAACTCCTGTGGAACTGCTGATATCAATTCACTGGCAA CTGACGGGAAACTCCTCAAACTCAACTCTCGATCCACACTGGCCATTGACTGGGACAGTGAGACCCGAAG CCTTTACTTTGATGAGCAAGAATCTGAGGCCTGTGAGAAGCACCTGAGCATGTCACAGCCGCAGAAGAAG AAGAAGGCGGCAGTAGCCCTGCGAGAGTGCATCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATG ACCCCTGGTACTGTCCCACCTGTAAGAAGCACCAGCAGGCAACAAAGAAGTTTGACTTGTGGTCTTTGCC CAAGATCCTGGTGGTTCACCTCAAGCGTTTCTCCTATAACAGATACTGGCGGGATAAACTTGACACCGTG GTGGAGTTCCCAGTCAGAGCTCTGAACATGTCCGAGTTTGTCTGTGACCGGTCAGCAAGGCCTTATGTTT ATGACCTAATTGCTGTGTCCAATCACTATGGAGCCATGGGGGTTGGTCACTACACTGCATATGCGAAGAA CAGACTGAACGGGAAATGGTATTACTTTGATGATAGCAGTGTGTCCCTGGCCTCTGAGGACCAGATAGTG ACGAAAGCCGCCTACGTGTTGTTCTATCAGCGTCGGGATGACGAATGCTCCAGCACATCTTCGCTCGGCA GTTTCCCCGGTTCTGATGGAGGGGTGAAGCTGAGCAGCTCACACCAGGGCATGGGGGATGAGGAGGCTTA CAACATGGACACCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226489 representing NM_011678
Red=Cloning site Green=Tags(s) MAEGRGSRERPDVETQKTELGALMGTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPID NSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLEL KLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQG QVLVIEPQNEDGTWPRQSLQSKSSTAPSRNFTTSSKPSASPYCSVSASLIANGDSTNSSGMHSSGVSRGG SGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTEYFLKDEYEAEINRDNPLGMKGE IAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKPYLEP KDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIME VFLVPADPQCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRD DIFVYEVCNTSMDGSECITLPVYFREKKSRPSSASSGAVLYGQPLLVSVPKHKLTLESLYQAVCDRISRY IKQPLPDEFLSSPLEPGACNGSRSSYEGDEEEEMDHQEEGKEQLSEVEGSGEDDQGDDHSESAQKVKGQP RHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSLYFDEQESEACEKHLSMSQPQKK KKAAVALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTV VEFPVRALNMSEFVCDRSARPYVYDLIAVSNHYGAMGVGHYTAYAKNRLNGKWYYFDDSSVSLASEDQIV TKAAYVLFYQRRDDECSSTSSLGSFPGSDGGVKLSSSHQGMGDEEAYNMDTN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011678 |
ORF Size | 2886 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011678.2, NP_035808.2 |
RefSeq Size | 3686 bp |
RefSeq ORF | 2889 bp |
Locus ID | 22258 |
UniProt ID | P35123 |
Gene Summary | Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222871 | Usp4 (untagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), (10ug) |
CNY 12,350.00 |
|
MR226489 | Usp4 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (Usp4) |
CNY 7,048.00 |
|
MR226489L3 | Lenti ORF clone of Usp4 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (Usp4) |
CNY 10,360.00 |
|
MR226489L4 | Lenti ORF clone of Usp4 (mGFP-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (Usp4) |
CNY 10,360.00 |