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Usp19 (NM_001168371) Mouse Tagged ORF Clone
CAT＃: MG226425
- TrueORF®
Usp19 (tGFP-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001168371" in other vectors (4)
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CNY 12,730.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 8430421I07Rik; AI047774; Zmynd9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226425 representing NM_001168371
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCAGGGGCCAGTGCTACAGGGCCCAGGAGGGGGCCGCCAGGACTGGAAGAGGCCACTAGTAAGA AGAAACAGAAGGATCGAGCAAACCTGGAAAGTAAAGATGGAGATGCTAGGAGAGTGTCCCTTCCTCGAAA GGAACCAACCAAAGATGAATTGTTGCTCGATTGGAGGCAGAGTGCAGATGAGGTGATTGTTAAGCTGCGC GTGGGAACAGGTCCCGTACGTCTGGAGGATGTAGATGCTGCGTTCACAGACACGGACTGTGTGGTGAGGC TTCCAGATGGTCGGCAGTGGGGTGGTGTCTTCTTTGCTGAAATACAAAGTTCTTGCACCAAAGTGCAGGC TCGCAAGGGTGGTCTTCTACAGCTAGTACTACCCAAGAAGGTGCCTCTGCTCACGTGGCCCTCTCTCCTG AAGAAACCTCTGGGAACCCAAGAGCTGGTGCCAGGTTTGCAGTGCCAGGAGAACGGGCAAGAGCTGTCTC CCATTGCCCTGGAGCCAGGCTCTGAGCCCCGCAGAGCTAAACAGGAAGCCCGAAACCAGAAGCGGGCCCA GGGCCGTGGTGAGGTAGGCTCGGGGGCTGGCCCTGGGACACAGGCAGGGCCCAGCGCCAAGAGGGCTGTT CACCTCCGCAGAGGGCCAGAAGGGGAAGGGTCCATGGATGGCCCCGGCCCCCAGGGTGATGCCCCGTCTT TCCTGTCTGACTCAGCTACCCAGGTTGAGGCTGAGGAGAAGCTCTGTGCTCCACCAATGAACACTCAAAC AAGTCTCTTGAGCTCAGAGAAGAGTTTAGCCCTTCTGACAGTAGAGAAGACAGTGTCCCCCAGGAATGAC CCAGTCGCCCCGGTTATGGTCCAGGACAGAGACCCTGAGCCTGAGCAAGAAGACCAAGTCAAAGAGGAGA TGGCACTTGGGGCTGATCCTACAGCCTTGGTGGAGGAACCAGAGTCTATGGTGAACCTGGCATTTGTCAA GAACGACTCGTATGAGAAGGGCCCGGATTCGGTGGTGGTGCACGTGTACGTGAAGGAGATCCGCAGGGAC AGCTCCCGAGTGCTCTTCCGAGAGCAGGACTTCACACTGATCTTCCAGACCAGGGACGGAAACTTTCTGA GGCTGCATCCGGGCTGTGGGCCCCACACCATCTTCCGATGGCAGGTGAAGCTCAGAAACTTGATTGAACC AGAGCAGTGTACGTTCTGTTTCACGGCCTCTCGAATCGATATCTGCCTCCGGAAGCGGCAGAGTCAGCGC TGGGGGGGACTGGAGGCCCCTGCTACACGAGGTGCAGTGGGTGGTGCAAAGGTTGCCGTGCCGACAGGCC CAACCCCTTTGGATTCAACCCCTCCAGGAGGTGGCCCCCACCCCCTGACAGGCCAGGAGGAAGCCAGGGC TGTGGAGAAGGAAAAACCCAAGGCTCGATCAGAGGACTCAGGGCTGGATGGTGTGGTGGCCCGCACCCCC TTGGAGCATGTAGCCCCAAAGCCAGACCCACACTTGGCCTCGCCCAAACCCACGTGTATGGTGCCTCCAA TGCCGCACAGTCCAGTTAGTGGGGATAGTGTGGAGGAGGACGAAGAGGAAGAGAAGAAGGTGTGCCTGCC AGGCTTCACTGGCCTTGTCAACTTAGGGAACACCTGCTTCATGAATAGCGTCATTCAGTCTTTGTCCAAC ACTCGGGAACTTCGTGACTTCTTTCACGACCGATCCTTTGAAGCTGAGATTAACTACAATAACCCATTGG GGACTGGTGGGCGCCTCGCCATTGGCTTTGCTGTGCTGCTCCGGGCCCTATGGAAGGGTACTCACCAAGC CTTTCAGCCCTCCAAGCTAAAGGCCATTGTGGCAAGCAAGGCCAGCCAGTTCACAGGCTATGCACAGCAT GATGCTCAAGAGTTCATGGCTTTCTTGTTGGATGGGCTACATGAAGACCTCAATCGAATCCAAAACAAAC CCTACACAGAGACTGTGGACTCGGACGGGCGGCCCGATGAGGTGGTAGCCGAGGAAGCATGGCAGCGGCA CAAGATGAGAAATGATTCATTCATTGTGGACCTGTTTCAGGGCCAGTACAAGTCAAAGCTGGTGTGCCCT GTGTGTGCCAAGGTCTCCATCACTTTTGACCCGTTCCTTTATCTGCCGGTACCCTTGCCACAAAAGCAAA AGGTTCTCCCCATATTTTATTTTGCCAGGGAGCCCCACAGCAAGCCCATCAAGTTCCTGGTGAGTGTCAG CAAGGAGAACTCCAGCGCGAGTGAAGTGTTGGACTCCCTCTCTCAGAGTGTCCACGTGAAGCCTGAGAAC CTGCGCCTAGCCGAGGTAATTAAGAACCGTTTCCACCGTGTTTTCTTGCCCTCCCACTCACTGGACGCTG TGTCCCCCACGGACGTGCTCCTCTGCTTTGAGCTGCTCTCCCCAGAACTGGCTAAGGAGCGGGTAGTAGT GCTGGAGGTGCAGCAGCGCCCCCAGGTACCCAGCATCCCTATCTCCAAGTGCGCAGCCTGCCAGCGGAAG CAGCAATCAGAAGAAGAAAAGCTGAAGCGCTGTACCCGTTGCTACCGTGTGGGCTACTGCAACCAGTTCT GCCAGAAAACCCATTGGCCTGACCACAAAGGCCTCTGCCGCCCTGAGAACATTGGCTACCCCTTCCTGGT CAGTGTGCCTGCTTCACGCCTCACTTATGCCCGTCTTGCTCAGCTACTAGAAGGTTATGCCCGGTACTCT GTGAGTGTATTCCAACCGCCCTTCCAGCCTGGCCGCATGGCTTTGGAATCGCAGAGCCCTGGCTGTACCA CGTTGCTTTCAACCAGCTCTCTGGAGGCTGGGGACAGTGAAAGAGAACCCATTCAGCCTTCTGAGCTCCA GCTGGTGACCCCTGTGGCTGAAGGGGATACAGGGGCTCACCGAGTATGGCCGCCTGCTGATAGGGGTCCT GTGCCTAGCACCAGTGGACTCTCTTCTGAGATGCTGGCCAGTGGGCCTATCGAAGGTTGTCCCTTGCTTG CTGGTGAGAGGGTATCTCGGCCTGAAGCTGCTGTGCCTGGGTACCAACACTCAAGTGAATCTGTGAATAC CCACACGCCCCAGTTCTTCATCTATAAAATTGATGCATCAAACCGTGAGCAGCGGCTGGAGGACAAAGGG GAGACACCATTGGAGCTAGGTGATGACTGTAGCCTGGCTCTGGTGTGGCGGAACAATGAACGCCTGCAGG AGTTTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAAGATCCAGGCTCTGCTGGTGAGGCTGCCCG TGCTGGCCACTTCACCCTGGACCAGTGCCTCAACCTCTTTACACGGCCTGAAGTGCTGGCACCTGAGGAG GCCTGGTACTGCCCACAGTGCAAACAGCATCGTGAGGCCTCCAAACAGCTGCTGTTGTGGCGCCTACCGA ACGTGCTGATTGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTCATTTGGCGAGACAAGATCAATGACTT GGTGGAGTTTCCTGTTCGGAACCTGGACTTGAGCAAGTTCTGTATCGGTCAGAAAGAGGAGCAGCTGCCT AGCTATGACCTGTATGCTGTCATCAACCACTACGGAGGCATGATCGGTGGCCACTATACTGCTTGTGCAC GGCTGCCCAATGATCGCAGTAGCCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACGGTGAC AACAGTAGACGAAAGCCAGGTCGTGACGCGCTATGCCTATGTTCTCTTCTACCGTCGTCGGAACTCTCCT GTGGAGAGACCCCCCAGGGCAAGTCACTCTGAACACCACCCAGACCTAGGCCCTGCAGCTGAGGCTGCTG CCAGCCAGGGACTAGGCCCTGGCCAGGCCCCCGAGGTGGCCCCCACGCGGACAGCCCCTGAACGCTTCGC CCCCCCTGTGGACCGCCCAGCCCCCACGTACAGCAACATGGAGGAGGTCGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226425 representing NM_001168371
Red=Cloning site Green=Tags(s) MSAGASATGPRRGPPGLEEATSKKKQKDRANLESKDGDARRVSLPRKEPTKDELLLDWRQSADEVIVKLR VGTGPVRLEDVDAAFTDTDCVVRLPDGRQWGGVFFAEIQSSCTKVQARKGGLLQLVLPKKVPLLTWPSLL KKPLGTQELVPGLQCQENGQELSPIALEPGSEPRRAKQEARNQKRAQGRGEVGSGAGPGTQAGPSAKRAV HLRRGPEGEGSMDGPGPQGDAPSFLSDSATQVEAEEKLCAPPMNTQTSLLSSEKSLALLTVEKTVSPRND PVAPVMVQDRDPEPEQEDQVKEEMALGADPTALVEEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEIRRD SSRVLFREQDFTLIFQTRDGNFLRLHPGCGPHTIFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQR WGGLEAPATRGAVGGAKVAVPTGPTPLDSTPPGGGPHPLTGQEEARAVEKEKPKARSEDSGLDGVVARTP LEHVAPKPDPHLASPKPTCMVPPMPHSPVSGDSVEEDEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSN TRELRDFFHDRSFEAEINYNNPLGTGGRLAIGFAVLLRALWKGTHQAFQPSKLKAIVASKASQFTGYAQH DAQEFMAFLLDGLHEDLNRIQNKPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCP VCAKVSITFDPFLYLPVPLPQKQKVLPIFYFAREPHSKPIKFLVSVSKENSSASEVLDSLSQSVHVKPEN LRLAEVIKNRFHRVFLPSHSLDAVSPTDVLLCFELLSPELAKERVVVLEVQQRPQVPSIPISKCAACQRK QQSEEEKLKRCTRCYRVGYCNQFCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYS VSVFQPPFQPGRMALESQSPGCTTLLSTSSLEAGDSEREPIQPSELQLVTPVAEGDTGAHRVWPPADRGP VPSTSGLSSEMLASGPIEGCPLLAGERVSRPEAAVPGYQHSSESVNTHTPQFFIYKIDASNREQRLEDKG ETPLELGDDCSLALVWRNNERLQEFVLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEE AWYCPQCKQHREASKQLLLWRLPNVLIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLP SYDLYAVINHYGGMIGGHYTACARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSP VERPPRASHSEHHPDLGPAAEAAASQGLGPGQAPEVAPTRTAPERFAPPVDRPAPTYSNMEEVD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001168371 |
| ORF Size | 3972 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001168371.2, NP_001161843.1 |
| RefSeq Size | 4728 bp |
| RefSeq ORF | 3975 bp |
| Locus ID | 71472 |
| UniProt ID | Q3UJD6 |
| Gene Summary | Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates (By similarity). Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing thier ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Exhibits a preference towards 'Lys-63'-linked ubiquitin chains (By similarity). Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224240 | Usp19 (untagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 2, (10ug) |
CNY 17,010.00 |
|
| MR226425 | Usp19 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 2 |
CNY 9,216.00 |
|
| MR226425L3 | Lenti ORF clone of Usp19 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 2 |
CNY 13,490.00 |
|
| MR226425L4 | Lenti ORF clone of Usp19 (mGFP-tagged) - Mouse ubiquitin specific peptidase 19 (Usp19), transcript variant 2 |
CNY 13,490.00 |
