Il15ra (NM_008358) Mouse Tagged ORF Clone

CAT#: MG226376

  • TrueORF®

Il15ra (tGFP-tagged) - Mouse interleukin 15 receptor alpha chain (Il15ra) transcript variant 1, (10ug)

ORF Plasmid: DDK tGFP



  "NM_008358" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,200.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Il15ra Antibody - N-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AA690181; IL-15RA
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG226376 representing NM_008358
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCGCCGCAGCTCCGGGGCTATGGAGTCCAGGCCATTCCTGTGTTGCTGCTGCTGCTGTTGCTAC
TGTTGCTCCCGCTGAGGGTGACGCCGGGCACCACGTGTCCACCTCCCGTATCTATTGAGCATGCTGACAT
CCGGGTCAAGAATTACAGTGTGAACTCCAGGGAGAGGTATGTCTGTAACTCTGGCTTTAAGCGGAAAGCT
GGAACATCCACCCTGATTGAGTGTGTGATCAACAAGAACACAAATGTTGCCCACTGGACAACTCCCAGCC
TCAAGTGCATCAGAGACCCCTCCCTAGCTCACTACAGTCCAGTGCCAACAGTAGTGACACCAAAGGTGAC
CTCACAGCCAGAGAGCCCCTCCCCCTCTGCAAAAGAGCCAGAAGCTTTCTCTCCCAAATCAGATACCGCA
ATGACCACAGAGACAGCTATTATGCCTGGCTCCAGGCTGACACCATCCCAAACAACTTCTGCAGGAACTA
CAGGGACAGGCAGTCACAAGTCCTCCCGAGCCCCATCTCTTGCAGCAACAATGACCTTGGAGCCTACAGC
CTCCACCTCCCTCAGGATAACAGAGATTTCTCCCCACAGTTCCAAAATGACGAAAGTGGCCATCTCTACA
TCGGTCCTCTTGGTTGGTGCAGGGGTTGTGATGGCTTTCCTGGCCTGGTACATCAAATCAAGGCAGCCTT
CTCAGCCGTGCCGTGTTGAGGTGGAAACCATGGAAACAGTACCAATGACTGTGAGGGCCAGCAGCAAGGA
GGATGAAGACACAGGAGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG226376 representing NM_008358
Red=Cloning site Green=Tags(s)

MASPQLRGYGVQAIPVLLLLLLLLLLPLRVTPGTTCPPPVSIEHADIRVKNYSVNSRERYVCNSGFKRKA
GTSTLIECVINKNTNVAHWTTPSLKCIRDPSLAHYSPVPTVVTPKVTSQPESPSPSAKEPEAFSPKSDTA
MTTETAIMPGSRLTPSQTTSAGTTGTGSHKSSRAPSLAATMTLEPTASTSLRITEISPHSSKMTKVAIST
SVLLVGAGVVMAFLAWYIKSRQPSQPCRVEVETMETVPMTVRASSKEDEDTGA

TRTRPLE - GFP Tag - V
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_008358
ORF Size 789 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008358.2
RefSeq Size 792 bp
RefSeq ORF 792 bp
Locus ID 16169
UniProt ID Q60819
Gene Summary High-affinity receptor for interleukin-15 (PubMed:17947230). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (PubMed:17947230). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (By similarity). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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