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Pag1 (NM_001195031) Mouse Tagged ORF Clone
CAT＃: MG226334
- TrueORF®
Pag1 (tGFP-tagged) - Mouse phosphoprotein associated with glycosphingolipid microdomains 1 (Pag1) transcript variant A, (10ug)
ORF Plasmid: DDK
"NM_001195031" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cbp; F730007C19Rik; Pag |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226334 representing NM_001195031
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCTGCAGGAAGCGTACTGAGCAGTGGGCAGATGCAGATGCAGATGGTCCTGTGGGGAAGTCTGG CTGCCGTGGCCATGTTCTTCCTCATCACCTTCCTCGTCCTCCTGTGTTCCACTTGCGACAGAGAGAAGAA GCCACGGCAGCATAGCGGGGACCATGAGAACCTGATGAATGTGCCTTCCGACAAGGACATGTTCAGCCAT TCGGCAACCAGCCTGACAACAGATGCACTGGCTAGCAGCGAACAGAACGGGGTGCTCACCAATGGCGACA TTCTTTCAGAAGACAGCACGCTGACCTGCATGCAGCACTATGAGGAAGTCCAGACCTCGGCTTCCGATCT CCTGGACTCCCAGGACAGCACAGGAAAGGCCAAGTGCCACCAGAGCCGGGAGCTGCCCAGGATCCCGCCT GAGAACGCCGTGGACGAGATTCTCACAGCCAGGGCTGCAGACACGGAACTGGGGCCCGGAGTGGAGGGGC CCTACGAGGTGCTCAAGGACAGCTCCTCCCAGGAGAACATGGTGGAGGACTGTCTGTATGAAACAGTGAA GGAGATCAAGGAAGTGGCAGACAAAGGCCAGGGTGGCAAGTCCAAGTCCACTTCTGCCTTGAAGGAGCTT CAGGGGGCTCCCATGGAGGGCAAGGCCGACTTTGCTGAATATGCCTCTGTGGACAGAAACAAAAAGTGCC GCCACAGCGCGAACGCAGAGAGCATTCTGGGAACTTGCAGTGACCTGGACGAGGAAAGCCCACCTCCTGT CCCGGTTAAGCTTCTGGATGAGAATGCCAACCTTCCCCAGGAGGGAGGAGGACAGGCAGAGGAGCAAGCT GCAGAAGGGACCGGTGGACACAGCAAGAGATTTAGTTCCTTGTCATACAAGTCTCGGGAAGAAGACCCGA CTCTTACAGAAGAGGAGATCTCAGCAATGTATTCCTCAGTGAATAAGCCTGGCCAGTCGGCACACAAACC TGGACCATGTATGAAAGGGCCAGAATCTGCCTGCCACTCCATGAAGGGCCTCCCTCAGAGGTCGTCATCT TCCTGCAATGACCTCTATGCCACTGTGAAAGACTTTGAGAAAACTCCCAACAGCATCAGCACACTGCCCC CGGCAAGGAGACCCAGTGAGGAGCCAGAGCCTGACTATGAAGCCATACAGACTCTAAACAGAGAGGATGA GAAGGTCCCTCTGGAGACCAATGGCCACCATGTCCCCAAGGAGAGTGACTACGAGAGCATTGGGGACTTG CAGCAATGCAGAGATGTCACCAGGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226334 representing NM_001195031
Red=Cloning site Green=Tags(s) MGPAGSVLSSGQMQMQMVLWGSLAAVAMFFLITFLVLLCSTCDREKKPRQHSGDHENLMNVPSDKDMFSH SATSLTTDALASSEQNGVLTNGDILSEDSTLTCMQHYEEVQTSASDLLDSQDSTGKAKCHQSRELPRIPP ENAVDEILTARAADTELGPGVEGPYEVLKDSSSQENMVEDCLYETVKEIKEVADKGQGGKSKSTSALKEL QGAPMEGKADFAEYASVDRNKKCRHSANAESILGTCSDLDEESPPPVPVKLLDENANLPQEGGGQAEEQA AEGTGGHSKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQSAHKPGPCMKGPESACHSMKGLPQRSSS SCNDLYATVKDFEKTPNSISTLPPARRPSEEPEPDYEAIQTLNREDEKVPLETNGHHVPKESDYESIGDL QQCRDVTRL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001195031 |
| ORF Size | 1287 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001195031.1, NP_001181960.1 |
| RefSeq Size | 8256 bp |
| RefSeq ORF | 1290 bp |
| Locus ID | 94212 |
| UniProt ID | Q3U1F9 |
| Gene Summary | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212009 | Pag1 (untagged) - Mouse phosphoprotein associated with glycosphingolipid microdomains 1 (Pag1), transcript variant A, (10ug) |
CNY 3,990.00 |
|
| MR226334 | Pag1 (Myc-DDK-tagged) - Mouse phosphoprotein associated with glycosphingolipid microdomains 1 (Pag1), transcript variant A |
CNY 3,656.00 |
|
| MR226334L3 | Lenti ORF clone of Pag1 (Myc-DDK-tagged) - Mouse phosphoprotein associated with glycosphingolipid microdomains 1 (Pag1), transcript variant A |
CNY 4,750.00 |
|
| MR226334L4 | Lenti ORF clone of Pag1 (mGFP-tagged) - Mouse phosphoprotein associated with glycosphingolipid microdomains 1 (Pag1), transcript variant A |
CNY 4,750.00 |
