Psmd10 (NM_001164177) Mouse Tagged ORF Clone
CAT#: MG226301
- TrueORF®
Psmd10 (tGFP-tagged) - Mouse proteasome (prosome macropain) 26S subunit non-ATPase 10 (Psmd10) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001164177" in other vectors (3)
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CNY 2,090.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AW554874 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226301 representing NM_001164177
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGTGTGTGTCTAACATAATGATCTGTAACCTGGCCTACAGTGGGAAGCTGGATGAGTTGAAGG AGCGCATTTTGGCTGATAAATCTCTGGCTACTAGAACTGATCAGGACAGCAGAACAGCTTTGCACTGGGC ATGCTCAGCTGGCCATACAGAAATTGTTGAATTCTTGCTGCAACTTGGAGTGCCAGTGAATGATAAAGAT GACGCAGGTTGGTCTCCTCTTCATATTGCTGCCTCCGCTGGCCGGGATGAGATTGTAAAAGCCCTTCTGG TGAAAGGTGCACATGTGAATGCTGTCAATCAAAACGGCTGCACTCCACTCCATTATGCAGCTTCGAAGAA TAGGCATGAGATTGCTGTTATGTTACTAGAAGGTGGGGCTAACCCAGATGCGAAGGACCATTACGATGCT ACAGCAATGCACCGGGCAGCAGCCAAGGACACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226301 representing NM_001164177
Red=Cloning site Green=Tags(s) MEGCVSNIMICNLAYSGKLDELKERILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKD DAGWSPLHIAASAGRDEIVKALLVKGAHVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYDA TAMHRAAAKDT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001164177 |
ORF Size | 453 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001164177.1, NP_001157649.1 |
RefSeq Size | 1377 bp |
RefSeq ORF | 456 bp |
Locus ID | 53380 |
UniProt ID | Q9Z2X2 |
Gene Summary | Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity). Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR226301 | Psmd10 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10), transcript variant 2 |
CNY 1,900.00 |
|
MR226301L3 | Lenti ORF clone of Psmd10 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10), transcript variant 2 |
CNY 3,800.00 |
|
MR226301L4 | Lenti ORF clone of Psmd10 (mGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (Psmd10), transcript variant 2 |
CNY 3,800.00 |