Rtn4 (NM_194051) Mouse Tagged ORF Clone
CAT#: MG226113
- TrueORF®
Rtn4 (tGFP-tagged) - Mouse reticulon 4 (Rtn4) transcript variant 5, (10ug)
ORF Plasmid: DDK
"NM_194051" in other vectors (4)
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CNY 11,500.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; mKIAA0886; mKIAA4153; NgA |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226113 representing NM_194051
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCCCCGCTGGCGGGAGGCGGGCAGAAGGGTGGGGCGGCCTCGGAAGCCTGGGTGCCTTCATTGT TTGTCGGGGTTTCGGGGAGCACGTGCACCGCTGCCAAGTCGCTGGTTCCCATTCCAGCACGAAGTTCTCG CCTATCAGCAGCTAGGAATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGATACCCTCCTCT GCAGAAAAAATTATGGATTTGAAGGAGCAGCCAGGTAACACTGTTTCGTCTGGTCAAGAGGATTTCCCAT CTGTCCTGTTTGAAACTGCTGCCTCTCTTCCTTCTCTATCTCCTCTCTCAACTGTTTCTTTTAAAGAACA CGGATACCTTGGTAACTTATCAGCAGTGGCATCCACAGAAGGAACTATTGAAGAAACTTTAAATGAAGCT TCTAGAGAATTGCCAGAGAGGGCAACAAATCCATTTGTAAATAGAGAGTCAGCAGAGTTTTCAGTATTAG AATACTCAGAAATGGGATCATCTTTCAATGGCTCCCCAAAAGGAGAGTCAGCCATGTTAGTAGAAAACAC TAAGGAAGAAGTAATTGTGAGGAGTAAAGACAAAGAGGATTTAGTTTGTAGTGCAGCCCTTCATAATCCA CAAGAGTCACCTGCGACCCTTACTAAAGTGGTTAAAGAAGACGGAGTTATGTCTCCAGAAAAGACAATGG ACATTTTTAATGAAATGAAAATGTCAGTGGTAGCACCTGTGAGGGAAGAGTATGCAGATTTTAAGCCATT TGAACAAGCATGGGAAGTGAAAGATACTTATGAGGGAAGTAGGGATGTGCTGGCTGCTAGAGCTAATATG GAAAGTAAAGTGGACAAAAAATGCTTTGAAGATAGCCTGGAGCAAAAAGGTCATGGGAAGGATAGTGAAA GCAGAAATGAGAATGCTTCTTTCCCCAGGACCCCAGAACTTGTGAAGGACGGCTCCAGAGCGTACATCAC CTGTGATTCCTTTAGCTCAGCAACCGAGAGTACTGCAGCAAACATTTTCCCTGTGCTAGAAGATCACACT TCAGAAAACAAAACAGATGAAAAAAAAATAGAAGAAAGGAAGGCCCAAATTATAACAGAGAAGACTAGCC CCAAAACGTCAAATCCTTTCCTTGTAGCAATACATGATTCTGAGGCAGATTATGTCACAACAGATAATTT ATCAAAGGTGACTGAGGCAGTAGTGGCAACCATGCCTGAAGGTCTAACGCCAGATTTAGTTCAGGAAGCA TGTGAAAGTGAACTGAACGAAGCCACAGGTACAAAGATTGCTTATGAAACAAAAGTGGACTTGGTCCAGA CATCAGAAGCTATACAAGAGTCAATTTACCCCACAGCACAGCTTTGCCCATCATTTGAGGAAGCTGAAGC AACTCCGTCACCAGTTTTGCCTGATATTGTTATGGAAGCGCCATTAAATTCTCTCCTTCCAAGCACTGGT GCTTCTGTAGCGCAGCCCAGTGCATCCCCACTAGAAGTACCGTCTCCAGTTAGTTATGACGGTATAAAGC TTGAGCCTGAAAATCCCCCACCATATGAAGAAGCCATGAGTGTAGCACTAAAAACATCGGACTCAAAGGA AGAAATTAAAGAGCCTGAAAGTTTTAATGCAGCTGCTCAGGAAGCAGAAGCTCCTTATATATCCATTGCA TGTGATTTAATTAAAGAAACAAAGCTCTCCACTGAGCCAAGTCCAGAGTTCTCTAATTATTCAGAAATAG CAAAATTTGAGAAGTCGGTGCCTGATCACTGTGAGCTCGTGGATGATTCCTCACCCGAATCTGAACCAGT TGACTTATTTAGTGATGATTCAATTCCTGAAGTCCCACAAACACAAGAGGAGGCTGTGATGCTAATGAAG GAGAGTCTCACTGAAGTGTCTGAGACAGTAACACAACACAAACATAAGGAGAGACTTAGTGCTTCACCTC AGGAGGTAGGAAAGCCATATTTAGAGTCTTTTCAGCCCAATTTACATATTACAAAAGATGCTGCATCTAA TGAAATTCCAACATTGACCAAAAAGGAGACAATTTCTTTGCAAATGGAAGAGTTTAATACTGCAATTTAT TCCAATGATGACTTACTTTCTTCTAAGGAAGACAAAATGAAAGAAAGTGAAACATTTTCCGATTCATCTC CCATTGAGATAATAGATGAGTTTCCCACATTTGTCAGTGCTAAAGATGATTCTCCTAAGGAGTACACTGA CCTAGAAGTATCCAACAAAAGTGAAATTGCTAATGTCCAGAGCGGGGCCAATTCGTTGCCTTGCTCAGAA TTGCCCTGTGACCTTTCTTTCAAGAATACATATCCTAAAGATGAAGCACATGTCTCAGATGAATTCTCCA AAAGTAGGTCCAGTGTATCTAAGGTGCCCTTATTGCTTCCAAATGTTTCTGCTTTGGAATCTCAAATAGA AATGGGCAACATAGTTAAACCCAAAGTACTTACGAAAGAAGCAGAGGAAAAACTTCCTTCTGATACAGAG AAAGAGGACAGATCCCTGACAGCTGTATTGTCAGCAGAGCTGAATAAAACTTCAGTTGTTGACCTCCTGT ACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCTTATTCCTGCTGCTGTCTCTGACAGT GTTCAGCATTGTCAGTGTAACGGCCTACATTGCCTTGGCCCTGCTCTCTGTGACTATCAGCTTTAGGATA TATAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATTTGGAATCTG AAGTTGCCATATCAGAGGAATTGGTTCAGAAATATAGTAATTCTGCTCTTGGTCATGTGAACAGCACAAT AAAAGAATTGAGGCGTCTCTTCTTAGTTGATGATTTAGTTGATTCCCTGAAGTTTGCAGTGTTGATGTGG GTATTTACTTACGTTGGTGCCTTGTTCAATGGTTTGACACTACTGATTTTAGCTCTGATCTCACTCTTCA GTATTCCTGTTATATATGAACGGCATCAGGCGCAGATAGATCATTATCTAGGACTTGCAAACAAGAGCGT TAAGGATGCCATGGCCAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226113 representing NM_194051
Red=Cloning site Green=Tags(s) MAPPLAGGGQKGGAASEAWVPSLFVGVSGSTCTAAKSLVPIPARSSRLSAARNETLFALPAASEPVIPSS AEKIMDLKEQPGNTVSSGQEDFPSVLFETAASLPSLSPLSTVSFKEHGYLGNLSAVASTEGTIEETLNEA SRELPERATNPFVNRESAEFSVLEYSEMGSSFNGSPKGESAMLVENTKEEVIVRSKDKEDLVCSAALHNP QESPATLTKVVKEDGVMSPEKTMDIFNEMKMSVVAPVREEYADFKPFEQAWEVKDTYEGSRDVLAARANM ESKVDKKCFEDSLEQKGHGKDSESRNENASFPRTPELVKDGSRAYITCDSFSSATESTAANIFPVLEDHT SENKTDEKKIEERKAQIITEKTSPKTSNPFLVAIHDSEADYVTTDNLSKVTEAVVATMPEGLTPDLVQEA CESELNEATGTKIAYETKVDLVQTSEAIQESIYPTAQLCPSFEEAEATPSPVLPDIVMEAPLNSLLPSTG ASVAQPSASPLEVPSPVSYDGIKLEPENPPPYEEAMSVALKTSDSKEEIKEPESFNAAAQEAEAPYISIA CDLIKETKLSTEPSPEFSNYSEIAKFEKSVPDHCELVDDSSPESEPVDLFSDDSIPEVPQTQEEAVMLMK ESLTEVSETVTQHKHKERLSASPQEVGKPYLESFQPNLHITKDAASNEIPTLTKKETISLQMEEFNTAIY SNDDLLSSKEDKMKESETFSDSSPIEIIDEFPTFVSAKDDSPKEYTDLEVSNKSEIANVQSGANSLPCSE LPCDLSFKNTYPKDEAHVSDEFSKSRSSVSKVPLLLPNVSALESQIEMGNIVKPKVLTKEAEEKLPSDTE KEDRSLTAVLSAELNKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRI YKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMW VFTYVGALFNGLTLLILALISLFSIPVIYERHQAQIDHYLGLANKSVKDAMAKIQAKIPGLKRKAE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_194051 |
ORF Size | 3138 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_194051.3, NP_918940.1 |
RefSeq Size | 5558 bp |
RefSeq ORF | 3141 bp |
Locus ID | 68585 |
UniProt ID | Q99P72 |
Gene Summary | Required to induce the formation and stabilization of endoplasmic reticulum (ER) tubules. They regulate membrane morphogenesis in the ER by promoting tubular ER production. They influence nuclear envelope expansion, nuclear pore complex formation and proper localization of inner nuclear membrane proteins. However each isoform have specific functions mainly depending on their tissue expression specificities.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223370 | Rtn4 (untagged) - Mouse reticulon 4 (Rtn4), transcript variant 5, (10ug) |
CNY 13,490.00 |
|
MR226113 | Rtn4 (Myc-DDK-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 5 |
CNY 10,450.00 |
|
MR226113L3 | Lenti ORF clone of Rtn4 (Myc-DDK-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 5 |
CNY 12,350.00 |
|
MR226113L4 | Lenti ORF clone of Rtn4 (mGFP-tagged) - Mouse reticulon 4 (Rtn4), transcript variant 5 |
CNY 12,350.00 |