Fbln1 (NM_010180) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG225912 representing NM_010180 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCCCCGTGCCGTCGCGCCTCGTCCCGCTGCCGCTGCTGCTGCTCAGCAGCCTCTCGCTGCTGG CAGCCCGAGCGAATGCAGACATCTCCATGGAGGCTTGCTGCACGGATGGAAATCAGATGGCTAACCAGCA CAGGGACTGCTCGCTGCCGTACACCTCAGAATCCAAGGAGTGCAGGATGGTCCAGGAGCAATGTTGTCAC AACCAACTGGAAGAGCTGCACTGTGCCACGGGCATCAACCTGGCCAGCGAGCCAGAAGGCTGCGCCTCGC TCCACAGCTACAACAGTAGCCTTGAGACCATCTTCATAAAGAGGTGCTGCCACTGTTGCATGCTGGGAAA GGCATCCCTGGCCCGAGACCAGACCTGTGAACCCATTGTCATGATAAGCTACCAGTGTGGGCTGGTGTTC CGTGCCTGCTGTGTGAAGGCCCGGGAGAATTCAGACTTTGTCCAAGGCAACGGTGCAGACCTTCAGGACC CAGCTAAGATTCCTGACGAGGAGGACCAAGAAGACCCGTACCTGAATGACCGCTGTCGAGGTGGCGGGCC CTGTAAGCAGCAGTGCCGTGACACTGGGGACGAGGTGATCTGCTCTTGCTTTGTGGGCTACCAGCTGCAG TCGGATGGTGTCTCCTGCGAAGATATCAATGAATGCATCACAGGCAGCCATAACTGCCGGCTGGGAGAAT CCTGCATCAATACAGTGGGCTCTTTCCGCTGCCAGCGGGACAGCAGCTGTGGGACTGGCTATGAGCTCAC AGAGGATAATAACTGCAAAGATATTGACGAATGTGAGACTGGTATTCATAACTGCCCCCCCGATTTTATC TGTCAGAATACTCTGGGATCCTTCCGTTGCAGACCGAAGCTGCAGTGCAAGAGCGGCTTCATACAGGATG CTCTAGGCAACTGCATTGATATCAATGAGTGTTTAAGTATCAGTGCTCCATGCCCTGTGGGGCAGACATG CATCAATACAGAGGGCTCCTACACATGCCAGAAGAATGTGCCCAACTGTGGCCGTGGTTATCATCTCAAT GAAGAGGGGACCCGCTGTGTTGATGTGGATGAGTGCTCCCCACCAGCGGAGCCCTGTGGGAAGGGACACC ACTGCCTGAACTCCCCCGGCAGCTTCCGCTGCGAGTGCAAGGCTGGCTTCTATTTTGATGGCATCAGCAG GACCTGCGTGGATATCAACGAGTGCCAGCGCTATCCCGGGCGCCTGTGTGGCCACAAGTGTGAGAACACG CCAGGCTCCTTCCACTGCAGCTGCTCCGCCGGCTTCCGGCTGTCTGTGGACGGCCGGTCTTGTGAAGATG TGAACGAGTGTCTCAACAGCCCTTGCAGCCAGGAGTGTGCTAATGTCTATGGCTCCTACCAGTGCTATTG CCGACGAGGTTACCAGCTCAGTGACGTGGATGGGGTCACCTGCGAAGATATTGATGAGTGTGCCCTGCCC ACTGGAGGTCACATCTGCTCCTACCGCTGCATCAACATCCCCGGAAGCTTCCAGTGCAGCTGCCCCTCAT CTGGCTACAGGCTAGCTCCCAATGGCCGCAACTGCCAAGACATTGATGAGTGTGTGACCGGCATCCATAA CTGTTCCATCAATGAGACTTGCTTCAACATCCAGGGCAGCTTCCGCTGTCTGTCCTTTGAATGCCCCGAG AACTATCGCCGCTCCGCAGACACCTTCCGCCAAGAGAAAACAGACACCGTCCGCTGCATCAAGTCTTGCC GTCCCAACGATGAGGCCTGCGTGCGGGACCCTGTACATACCGTCTCCCACACCGTCATCTCGCTGCCCAC CTTTCGAGAGTTCACCCGTCCTGAGGAGATCATCTTTCTGAGGGCTGTCACACCGCTGTACCCCGCCAAC CAGGCCGACATCATCTTCGACATCACAGAAGGGAACCTTCGAGACTCCTTTGATATCATCAAGCGCTATG AGGACGGCATGACTGTGGGTGTCGTGCGCCAAGTGCGGCCCATCGTGGGCCCGTTTTACGCTGTCCTGAA GCTGGAGATGAACTACGTGTTGGGAGGCGTAGTTTCCCACCGGAACGTCGTCAATGTACACATCTTCGTC TCCGAGTACTGGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG225912 representing NM_010180 Red=Cloning site Green=Tags(s) MERPVPSRLVPLPLLLLSSLSLLAARANADISMEACCTDGNQMANQHRDCSLPYTSESKECRMVQEQCCH NQLEELHCATGINLASEPEGCASLHSYNSSLETIFIKRCCHCCMLGKASLARDQTCEPIVMISYQCGLVF RACCVKARENSDFVQGNGADLQDPAKIPDEEDQEDPYLNDRCRGGGPCKQQCRDTGDEVICSCFVGYQLQ SDGVSCEDINECITGSHNCRLGESCINTVGSFRCQRDSSCGTGYELTEDNNCKDIDECETGIHNCPPDFI CQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPVGQTCINTEGSYTCQKNVPNCGRGYHLN EEGTRCVDVDECSPPAEPCGKGHHCLNSPGSFRCECKAGFYFDGISRTCVDINECQRYPGRLCGHKCENT PGSFHCSCSAGFRLSVDGRSCEDVNECLNSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALP TGGHICSYRCINIPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGSFRCLSFECPE NYRRSADTFRQEKTDTVRCIKSCRPNDEACVRDPVHTVSHTVISLPTFREFTRPEEIIFLRAVTPLYPAN QADIIFDITEGNLRDSFDIIKRYEDGMTVGVVRQVRPIVGPFYAVLKLEMNYVLGGVVSHRNVVNVHIFV SEYWF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_010180
ORF Size	2115 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_010180.2, NP_034310.2
RefSeq Size	3359 bp
RefSeq ORF	2118 bp
Locus ID	14114
UniProt ID	Q08879
Gene Summary	Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.[UniProtKB/Swiss-Prot Function]

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