Mylk3 (NM_175441) Mouse Tagged ORF Clone

CAT＃: MG225821

TrueORF^®

Mylk3 (tGFP-tagged) - Mouse myosin light chain kinase 3 (Mylk3), (10ug)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_175441" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,370.00

货期*

4周

规格

Product images

经常一起买 (4)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	D830007F02Rik; MLCK
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG225821 representing NM_175441 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGAGTTTCAGAGGAGGACCCAGAGGGTCTGGCCCCCCAGGGTCTGCCAGCATTGGGCGGAGCCT GCTTAGCCACCATGGACAAAAAACTTAATGTGCTGACTGAGAAGGTCGACAGACTCTTGCATTTCCAAGA AGATGTCACAGAGAAGCTGCAGTGTGTGTGCCAAGGCATGGATCACCTGGAACAAGATCTGCATCGGCTG GAGGCCTCCCGGGAGTTGAGTCTGGCAGGGTCTGGCAGCACTCCCCCAACCACGGCTCAGGCCGCATGGC CTGAGGTCCTGGAGCTGGTGAGGGCTGTGCGGCAGGAGGGTGCCCAGCATGGTGCCAGGCTCGAAGCCCT CTTCAAGATGGTGGTGGCTGTGGACAGGGCTATTACTTTGGTAGGGTCCACATTCCAGAATTCCAAGGTG GCTGATTTCATCATGCAAGGAACCGTGCCCGGGAGAAAAGGCAGTCTGGCCGATGGCCCGGAGGAGAACA AGGAGCAAGCAGAAGTTGCTGGAGTGAAGCCAAACCATGTACTGACTACAGGAGGTGTGCAAGCTGACGC CTCTAGGACGCTGTGGGAAGAGAGCCAAAAGGAGGACATACCTGTGCGAACAGTGGAGGGGCTGCCTCTC ATCATCAATACGTCACTGAAGGGAGCTGACCTAACCCAGGCAGGAGCCTCACTGAGGCAGGGAGTTGAAG TTCTTGGCCCAGGCCAAGTACCCCTACCCACAGAAGCAGAATCCAGGCTTCCTGAGACAGCCAGTGAGAA CACTGGAGCCACCCTGGAATTGTCCGTAGCAATCGACAGAATCAGCGAGGTCCTCACTAGCCTCAAGATG TCACAAGGTGGTGGTCAAGAAACCTCATCCAGCAAGCCTGACTGTTGGCTTTCAGAAGAGGCCATGAGGC TGAGTTCAGGGCCTCTCCCTCAGCCCCTAGGACCACTAACTCCAGACAGTGACATTCACAGTGGTGATGC ACTTCCCAGGATCCCTATCAATATGCAAGAGATGGCTACTCCTGGGGAGTTGCTTGAGACCCAAAGTGGC AGTCCCATTGGCTCTGCAGAAGCTCCAGGCCTTGGGACTGTGTTAGAAGACCAGATCCCTAAAGGAGCCA GACCATTTCCACCCCTGCCAAAGAGGAGCAGCAACAATGGTGGCATGAGTGCAGAGGAGGAGATAGGGTC TGGGGCTGAGCCTATGAGAGGACCAAGCTTGGCTACAAGGGACTGGAGAGATGAGACTGTTGGGACCACA GACCTGCAGCAAGGCATAGACCCAGGAGCAGTGAGCCCTGAGCCTGGGAAGGACCACGCAGCCCAGGGCC CAGGAAGAACTGAAGCTGGAAGGCTATCTTCTGCTGCAGAGGCTGCCATTGTGGTTCTAGATGACAGCGC AGCACCCCCAGCCCCTTTTGAACACCGGGTAGTGAGCATCAAAGATACCCTGATCTCAGCAGGCTACACG GTATCCCAACATGAAGTCTTAGGAGGGGGTCGGTTTGGCCAGGTGCACAGGTGTACAGAGAGGTCTACAG GCCTTGCACTGGCAGCCAAGATCATCAAAGTGAAGAACGTAAAGGACCGGGAGGATGTGAAGAATGAGGT CAACATCATGAACCAGCTCAGCCACGTAAACTTGATCCAACTTTATGATGCGTTTGAGAGCAAGAGCAGC TTCACTCTGATCATGGAGTATGTGGATGGAGGCGAACTCTTTGACCGGATCACGGATGAGAAGTACCACC TCACTGAGTTGGATGTGGTCTTGTTCACGAGGCAGATCTGTGAGGGTGTGCATTACCTGCATCAGCACTA TATCCTGCACCTGGACCTCAAGCCTGAGAACATATTGTGTGTCAGCCAGACAGGGCATCAAATTAAGATC ATTGACTTTGGGCTGGCTAGAAGATACAAGCCTCGGGAGAAGCTAAAGGTGAACTTTGGTACTCCGGAGT TCCTGGCCCCAGAAGTTGTTAACTATGAGTTTGTGTCATTTCCAACAGACATGTGGAGTGTGGGAGTTAT CACCTACATGCTACTCAGTGGTTTGTCCCCATTTCTAGGGGAGACAGATGCAGAGACCATGAATTTTATT GTGAACTGCAGCTGGGATTTCGATGCTGATACCTTCAAAGGGCTGTCGGAGGAAGCCAAGGACTTTGTTT CCCGGTTACTGGTCAAAGAGAAGAGCTGTAGGATGAGCGCCACACAGTGCCTGAAACACGAGTGGTTAAG TCACCTGCCTGCCAAAGCCTCGGGCTCCAACGTTCGCCTCAGATCCCAACAACTGCTGCAGAAATATATG GCTCAGAGTAAATGGAAGAAACATTTCCACGTGGTGACTGCAGTCAACAGGCTACGGAAATTTCCAACGT GTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG225821 representing NM_175441 Red=Cloning site Green=Tags(s) MSGVSEEDPEGLAPQGLPALGGACLATMDKKLNVLTEKVDRLLHFQEDVTEKLQCVCQGMDHLEQDLHRL EASRELSLAGSGSTPPTTAQAAWPEVLELVRAVRQEGAQHGARLEALFKMVVAVDRAITLVGSTFQNSKV ADFIMQGTVPGRKGSLADGPEENKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVRTVEGLPL IINTSLKGADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISEVLTSLKM SQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPINMQEMATPGELLETQSG SPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAEEEIGSGAEPMRGPSLATRDWRDETVGTT DLQQGIDPGAVSPEPGKDHAAQGPGRTEAGRLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISAGYT VSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYDAFESKSS FTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKI IDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFI VNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLSHLPAKASGSNVRLRSQQLLQKYM AQSKWKKHFHVVTAVNRLRKFPTCP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_175441
ORF Size	2385 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175441.5, NP_780650.2
RefSeq Size	3070 bp
RefSeq ORF	2388 bp
Locus ID	213435
UniProt ID	Q3UIZ8
Gene Summary	Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221728	Mylk3 (untagged) - Mouse myosin light chain kinase 3 (Mylk3), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 10,260.00
MR225821	Mylk3 (Myc-DDK-tagged) - Mouse myosin light chain kinase 3 (Mylk3)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,824.00
MR225821L3	Lenti ORF clone of Mylk3 (Myc-DDK-tagged) - Mouse myosin light chain kinase 3 (Mylk3)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
MR225821L4	Lenti ORF clone of Mylk3 (mGFP-tagged) - Mouse myosin light chain kinase 3 (Mylk3)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00

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