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Rictor (NM_030168) Mouse Tagged ORF Clone
CAT＃: MG225754
- TrueORF®
Rictor (tGFP-tagged) - Mouse RPTOR independent companion of MTOR complex 2 (Rictor), (10ug)
ORF Plasmid: DDK
"NM_030168" in other vectors (2)
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CNY 20,620.00
货期*
9周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225754 representing NM_030168
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGATCGGCCGCGGCCGCTCTCTGAAGAACCTCCGAATACGAGGGCGGAATGACAGCGGCGAGG AGAACGTCCCGCTCGATCTGACCCGAGAACCTTCTGATAACTTGAGGGAGATTCTCCAAAATGTGGCCAA ATTGCAAGGAGTATCAAATATGAGAAAGCTGGGCCATCTGAATAACTTCACTAAGCTTCTTTGCGATATT GGCCATAGTGAAGAAAAACTGGGCTTTAATTATGAAGACATCATAATTTGTTTGCGCTTAGCTTTATTAA ATGAGGCAAAAGAAGTAAGAGCAGCCGGGTTGCGAGCGCTGCGCTATCTCATCCAAGATTCCAGTATTCT CCAGAAGGTGCTAAAACTGAAAGTGGACTATTTAATAGCTAGGTGCATTGACATTCAGCAGAGCAACGAG GTGGAGAGGACACAAGCCCTTCGCTTAGTCAGAAAGATGATTACTGTGAATGCTTCCTTGTTTCCTAGTT CTGTGGCCAACTCATTAATTGCGGTTGGAAATGATGGGCTTCAGGAACGAGACAGAATGGTCCGGGCTTG CATCGCCATCATCTGTGAACTAGCACTTCAGAACCCAGAGGTGGTAGCCCTTCGAGGAGGACTAAACACC ATACTGAAAAATGTGATTGATTGCCAATTAAGTCGAATAAATGAGGCTCTCATTACTACAATTTTGCACC TTCTTAATCATCCAAAGACCCGGCAGTATGTGCGAGCTGATGTAGAATTAGAGCGAATTTTAGCACCCTA TACTGATTTCCACTACAGACACAGTCCAGATACGGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGA TTTCTAGCCAGTAAGATGGGAATCATTGCGACATTCCGCTCGTGGGCAGGTATTATTAATTTATGTAAGC CTGGAAATTCTGGGATACAGTCTCTAATTGGAGTACTTTGCATACCGAATATGGAAATAAGGCGAGGTCT GCTCGAAGTGCTCTATGACATCTTTCGTCTCCCTCTGCCCGTTGTGACGGATGAGTTCATAGAAGCCCTG CTCAGTGTAGATCCAGGAAGATTCCAGGACAGTTGGAGGCTTTCCGATGGCTTTGTGGCAGCTGAGGCAA AAACTATTCTTCCCCATCGTGCTAGATCCAGGCCAGACCTCATGGACAATTATTTGGCTCTGATACTCTC TGCATTTATTCGTAATGGACTTTTAGAGGGCTTGGTCGAGGTGATAACGAACAGTGACGACCACATCTCA GTGAGGGCGACCATCCTTTTAGGAGAGCTCCTGCATATGGCAAACACCATCCTGCCTCACTCGCATAGCC ACCATCTACACTGCTTGCCAACCCTCATGAACATGGCTGCGTCCTTCGATATTCCCAAGGAAAAGAGGCT GCGAGCCAGTGCAGCCCTGAACTGTTTGAACCGCTTCCATGAGATGAAGAAGCGAGGACCTAAGCCCTAC AGCCTTCATTTAGATCACATCATTCAGAAAGCAATCGCAACTCACCACAAGCGGGATCAGTATCTTCGAG TTCAGAAAGATATATTTGTTCTTAAGGATACAGAGGAAGCTCTTTTAATAAACCTTAGAGACAGCCAAGT CCTTCAGCATAAAGAGAATCTTGACTGGGATTGGAATCTGATTGGGACCATCCTTAAGTGGCCAAATGTA AATCTAAGAAACTATAAAGATGAGCAGTTGCACAGGTTTGTGCGCAGACTTCTTTACTTTTACAAGCCCA GCAGCAAACTGTACGCTAGTCTGGATCTGGACTTGGCCAAGTCCAAGCAGCTCACAGTTGTTGGTTGTCA GTTTACAGAATTTCTGCTCGAGTCTGAAGAGGATGGGCAAGGATACTTAGAAGATCTCGTGAAAGATATT GTTCAGTGGCTCAATGCTTCATCTGGAGTGAAACCTGAGAGAAGCCTTCAGAATAATGGCTTACTGACCA CCCTTAGTCAACACTACTTCTTATTTATCGGAACACTCTCTTGTCATCCTCATGGAGTCAAAATGCTGGA AAAGTGCAGTGTCTTTCAGTGTCTCCTTAATCTTTGTTCCTTGAAAAACCAAGATCATCTGATAAAACTC ACTGTTTCCAGCCTAGACTATAGCAGAGATGGATTAGCCAGAGTCATTCTTTCAAAAATCCTCACGGCAG CCACAGATGCTTGCAGACTGTATGCAACAAAACATTTAAGGGTTTTATTGAGAGCTAACGTTGAATTCTT CAACAATTGGGGAATTGAGTTACTAGTAACTCAGCTACATGATAAAAACAAAACAATTTCTTCTGAAGCG CTGGATATTCTTGATGAAGCTTGTGAAGACAAGGCCAATCTTCATGCTCTTATTCAGATGAAGCCAGCCT TATCCCACCTTGGAGACAAGGGCTTGCTTCTACTGCTGAGATTTCTTTCCATTCCGAAAGGATTTTCTTA CCTGAATGAAAGGGGTTATGTTGCAAAACAGTTGGAAAAGTGGCACAAGGAATATAATTCAAAGTATGTT GACTTGATTGAGGAACAACTTAATGAAGCCCTCACTACATACAGGAAGCCTATTGATGGTGATAACTACG TTCGTCGCAGTAATCAAAGACTACAGCGACCTCATGTCTACCTGCCCGTACACCTTTATGGACAACTGGT ACACCACAAAACAGGCTGCCATTTACTGGAAGTACAGAGTATCATCACAGAGCTCTGTCACAATGTTCGC ACTCCAGACTTGGACAAATGGGAAGACATTAAGAAGCTGAAAGCCTCTCTTTGGGCCTTGGGAAATATTG GCTCATCGAATTGGGGGCTTAATTTGCTACAGGAAGAAAATGTGATCCCAGATATACTAAAACTTGCAAA ACAGTGTGAGGTCCTTTCCATCCGAGGGACCTGTGTATATGTTCTTGGGCTCATAGCTAAAACCAAGCAA GGCTGTGATATTTTGAAATGTCACAGCTGGGATTCTGTAAGGCACAGTCGCAAGCATCTATGGCCAGTGG TTCCAGATGATGTGGAGCAGCTCTGCAATGAGCTCTCATCTGTCCCAAGCACCCTCAGCTTGAACTCTGA GTCGACCAGCTCTAGACATAACAGTGAGAGTGAGTCTGCACCATCAAGCATGTTCATGCTGGAGGACGAC CGCTTCGGCAGCACCTCCACCAGCACATTTTTCCTTGACATCAATGAAGATGCTGAGCCAGCATTTTATG ACCGACCTGGACCCATAAAAGATAAAAATTCATTTCCCTTTTTTGGCTCCAGCAAACTTGTAAAGAATCG TATCTTAAATTCACTTACTTTGCCTACTAAAAAACACCGCAGTAGCAGTGATCCAAAAGGAGGAAAACTG TCATCTGAAAATAAGACAAGCAACCGGCGAATCAGAACACTTACGGAGCCCAGTGTTGACTTAATCACAG CGAAGACTTCACAAGCTCCTCAGCACAGAAATCATTACAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225754 representing NM_030168
Red=Cloning site Green=Tags(s) MAAIGRGRSLKNLRIRGRNDSGEENVPLDLTREPSDNLREILQNVAKLQGVSNMRKLGHLNNFTKLLCDI GHSEEKLGFNYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNE VERTQALRLVRKMITVNASLFPSSVANSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNT ILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREAR FLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTDEFIEAL LSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHIS VRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLNRFHEMKKRGPKPY SLHLDHIIQKAIATHHKRDQYLRVQKDIFVLKDTEEALLINLRDSQVLQHKENLDWDWNLIGTILKWPNV NLRNYKDEQLHRFVRRLLYFYKPSSKLYASLDLDLAKSKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDI VQWLNASSGVKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLIKL TVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEA LDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHKEYNSKYV DLIEEQLNEALTTYRKPIDGDNYVRRSNQRLQRPHVYLPVHLYGQLVHHKTGCHLLEVQSIITELCHNVR TPDLDKWEDIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQ GCDILKCHSWDSVRHSRKHLWPVVPDDVEQLCNELSSVPSTLSLNSESTSSRHNSESESAPSSMFMLEDD RFGSTSTSTFFLDINEDAEPAFYDRPGPIKDKNSFPFFGSSKLVKNRILNSLTLPTKKHRSSSDPKGGKL SSENKTSNRRIRTLTEPSVDLITAKTSQAPQHRNHYS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030168 |
| ORF Size | 5124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030168.3, NP_084444.3 |
| RefSeq Size | 9328 bp |
| RefSeq ORF | 5127 bp |
| Locus ID | 78757 |
| UniProt ID | Q6QI06 |
| Gene Summary | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.[UniProtKB/Swiss-Prot Function] |
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