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Gabra2 (NM_008066) Mouse Tagged ORF Clone
CAT＃: MG225561
- TrueORF®
Gabra2 (tGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor subunit alpha 2 (Gabra2), (10ug)
ORF Plasmid: DDK
"NM_008066" in other vectors (4)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C630048P16Rik; Gabra-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225561 representing NM_008066
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACAAAATTGAGCACATGCAATGTATGGTCTCTGCTGCTTGTTCTTCTGGTGTGGGACCCAGTCA GGTTGGTGCTGGCTAACATCCAAGAAGATGAGGCTAAAAATAACATCACCATCTTTACAAGAATTCTAGA CAGACTTCTGGATGGTTATGATAATCGGCTTAGACCAGGACTGGGAGACAGTATTACTGAAGTCTTCACT AACATTTACGTGACCAGTTTTGGCCCTGTCTCAGATACAGATATGGAGTATACAATAGATGTTTTCTTTC GGCAAAAATGGAAAGATGAGCGTTTAAAATTTAAAGGTCCTATGAATATCCTTCGACTTAACAATTTAAT GGCCAGCAAAATCTGGACTCCTGATACCTTCTTTCACAATGGGAAAAAGTCAGTGGCCCATAACATGACA ATGCCAAACAAGCTGCTTCGAATCCAGGATGATGGAACATTGCTATATACCATGAGGCTTACAGTCCAAG CCGAATGTCCCATGCACCTGGAGGATTTCCCGATGGATGCTCATTCATGCCCACTGAAATTTGGAAGCTA CGCTTACACAACCTCAGAAGTCACATATATTTGGACTTACAATGCTTCTGACTCCGTTCAGGTTGCTCCC GATGGCTCCAGGTTAAATCAGTATGATTTGCTGGGCCAGTCAATTGGGAAGGAAACAATTAAATCCAGTA CAGGTGAATATACTGTAATGACAGCTCATTTCCACTTGAAAAGAAAAATTGGGTATTTTGTGATTCAGAC CTATCTGCCTTGCATCATGACTGTCATTCTCTCCCAAGTGTCATTCTGGCTGAACAGAGAATCGGTGCCA GCAAGAACTGTGTTTGGAGTAACAACTGTTCTAACAATGACAACCTTGAGCATCAGTGCTCGAAATTCCC TCCCGAAAGTGGCTTATGCCACTGCCATGGACTGGTTTATAGCTGTTTGTTATGCGTTTGTGTTCTCTGC CCTAATTGAATTTGCAACCGTGAATTACTTCACAAAAAGAGGATGGGCTTGGGACGGGAAGAGTGTAGTC AATGACAAGAAAAAAGAGAAAGGCTCCGTCATGATACAGAACAACGCCTATGCGGTAGCTGTTGCCAACT ATGCCCCGAATCTTTCCAAAGATCCTGTCCTCTCTACCATTTCCAAAAGTGCAACGACGCCAGAACCGAA CAAGAAGCCAGAGAACAAGCCAGCTGAAGCCAAGAAAACCTTCAACAGTGTCAGCAAAATCGACAGGATG TCTAGAATAGTGTTCCCAGTTCTGTTTGGTACTTTTAATTTAGTTTATTGGGCTACCTATTTAAACAGGG AGCCTGTATTAGGGGTCAGTCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225561 representing NM_008066
Red=Cloning site Green=Tags(s) MKTKLSTCNVWSLLLVLLVWDPVRLVLANIQEDEAKNNITIFTRILDRLLDGYDNRLRPGLGDSITEVFT NIYVTSFGPVSDTDMEYTIDVFFRQKWKDERLKFKGPMNILRLNNLMASKIWTPDTFFHNGKKSVAHNMT MPNKLLRIQDDGTLLYTMRLTVQAECPMHLEDFPMDAHSCPLKFGSYAYTTSEVTYIWTYNASDSVQVAP DGSRLNQYDLLGQSIGKETIKSSTGEYTVMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVP ARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWAWDGKSVV NDKKKEKGSVMIQNNAYAVAVANYAPNLSKDPVLSTISKSATTPEPNKKPENKPAEAKKTFNSVSKIDRM SRIVFPVLFGTFNLVYWATYLNREPVLGVSP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008066 |
| ORF Size | 1353 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008066.4 |
| RefSeq Size | 2392 bp |
| RefSeq ORF | 1356 bp |
| Locus ID | 14395 |
| UniProt ID | P26048 |
| Gene Summary | Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:27129275). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha2/beta2/gamma2 receptor exhibits synaptogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (PubMed:27129275).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215935 | Gabra2 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2 (Gabra2), (10ug) |
CNY 5,800.00 |
|
| MR225561 | Gabra2 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2 (Gabra2) |
CNY 5,488.00 |
|
| MR225561L3 | Lenti ORF clone of Gabra2 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2 (Gabra2) |
CNY 7,888.00 |
|
| MR225561L4 | Lenti ORF clone of Gabra2 (mGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2 (Gabra2) |
CNY 7,888.00 |
