Bmp4 (NM_007554) Mouse Tagged ORF Clone

CAT#: MG225467

  • TrueORF®

Bmp4 (tGFP-tagged) - Mouse bone morphogenetic protein 4 (Bmp4), (10ug)

ORF Plasmid: DDK tGFP



  "NM_007554" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,088.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Bmp-; Bmp-4; Bmp2; Bmp2b; Bmp2b-; Bmp2b-1; Bmp2b1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG225467 representing NM_007554
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCCTGGTAACCGAATGCTGATGGTCGTTTTATTATGCCAAGTCCTGCTAGGAGGCGCGAGCCATG
CTAGTTTGATACCTGAGACCGGGAAGAAAAAAGTCGCCGAGATTCAGGGCCACGCGGGAGGACGCCGCTC
AGGGCAGAGCCATGAGCTCCTGCGGGACTTCGAGGCGACACTTCTACAGATGTTTGGGCTGCGCCGCCGT
CCGCAGCCTAGCAAGAGCGCCGTCATTCCGGATTACATGAGGGATCTTTACCGGCTCCAGTCTGGGGAGG
AGGAGGAGGAAGAGCAGAGCCAGGGAACCGGGCTTGAGTACCCGGAGCGTCCCGCCAGCCGAGCCAACAC
TGTGAGGAGTTTCCATCACGAAGAACATCTGGAGAACATCCCAGGGACCAGTGAGAGCTCTGCTTTTCGT
TTCCTCTTCAACCTCAGCAGCATCCCAGAAAATGAGGTGATCTCCTCGGCAGAGCTCCGGCTCTTTCGGG
AGCAGGTGGACCAGGGCCCTGACTGGGAACAGGGCTTCCACCGTATAAACATTTATGAGGTTATGAAGCC
CCCAGCAGAAATGGTTCCTGGACACCTCATCACACGACTACTGGACACCAGACTAGTCCATCACAATGTG
ACACGGTGGGAAACTTTCGATGTGAGCCCTGCAGTCCTTCGCTGGACCCGGGAAAAGCAACCCAATTATG
GGCTGGCCATTGAGGTGACTCACCTCCACCAGACACGGACCCACCAGGGCCAGCATGTCAGAATCAGCCG
ATCGTTACCTCAAGGGAGTGGAGATTGGGCCCAACTCCGGCCCCTCCTGGTCACTTTTGGCCATGATGGC
CGGGGCCATACCTTGACCCGCAGGAGGGCCAAACGTAGTCCCAAGCATCACCCACAGCGGTCCAGGAAGA
AGAATAAGAACTGCCGTCGCCATTCACTATACGTGGACTTCAGTGACGTGGGCTGGAATGATTGGATTGT
GGCCCCACCCGGCTACCAGGCCTTCTACTGCCATGGGGACTGTCCCTTTCCACTGGCTGATCACCTCAAC
TCAACCAACCATGCCATTGTGCAGACCCTAGTCAACTCTGTTAATTCTAGTATCCCTAAGGCCTGTTGTG
TCCCCACTGAACTGAGTGCCATTTCCATGTTGTACCTGGATGAGTATGACAAGGTGGTGTTGAAAAATTA
TCAGGAGATGGTGGTAGAGGGGTGTGGATGCCGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG225467 representing NM_007554
Red=Cloning site Green=Tags(s)

MIPGNRMLMVVLLCQVLLGGASHASLIPETGKKKVAEIQGHAGGRRSGQSHELLRDFEATLLQMFGLRRR
PQPSKSAVIPDYMRDLYRLQSGEEEEEEQSQGTGLEYPERPASRANTVRSFHHEEHLENIPGTSESSAFR
FLFNLSSIPENEVISSAELRLFREQVDQGPDWEQGFHRINIYEVMKPPAEMVPGHLITRLLDTRLVHHNV
TRWETFDVSPAVLRWTREKQPNYGLAIEVTHLHQTRTHQGQHVRISRSLPQGSGDWAQLRPLLVTFGHDG
RGHTLTRRRAKRSPKHHPQRSRKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLN
STNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007554
ORF Size 1224 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007554.2, NP_031580.2
RefSeq Size 1994 bp
RefSeq ORF 1227 bp
Locus ID 12159
UniProt ID P21275
Gene Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Homozygous knockout mice die in utero, while a conditional knockout mouse exhibits defects in heart development. Transgenic mice overexpressing this gene in a neuron-specific manner exhibit a phenotype resembling the rare hereditary connective tissue disease, fibrodysplasia ossificans progressiva. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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