Col1a2 (NM_007743) Mouse Tagged ORF Clone
CAT#: MG225252
- TrueORF®
Col1a2 (tGFP-tagged) - Mouse collagen type I alpha 2 (Col1a2), (10ug)
ORF Plasmid: DDK
"NM_007743" in other vectors (2)
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CNY 15,010.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AA960264; AI325291; Co; Col; Col1; Col1a-2; Cola-2; Cola2; oim |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225252 representing NM_007743
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGCTTTGTGGATACGCGGACTCTGTTGCTGCTTGCAGTAACTTCGTGCCTAGCAACATGCCAAT ATTTGCAATCGGGATCAGTACGAAAGGGCCCCACTGGAGACAGAGGACCACGTGGACAAAGGGGTCCCGC AGGTCCCCGAGGCAGAGATGGTGTTGATGGTCCCATGGGCCCTCCTGGTCCCCCTGGCTCCCCTGGTCCT CCCGGCTCCCCTGCCCCCCCTGGTCTTACTGGGAACTTTGCTGCTCAGTATTCTGACAAAGGAGTTTCAT CTGGCCCTGGACCAATGGGTTTAATGGGACCCAGAGGCCCTCCTGGTGCTGTTGGAGCCCCTGGCCCTCA AGGTTTCCAAGGACCTGCTGGTGAACCTGGTGAGCCTGGTCAAACGGGTCCTGCAGGTCCCCGTGGTCCA GCTGGCTCTCCTGGCAAGGCTGGTGAGGATGGTCACCCTGGAAAACCCGGAAGACCTGGGGAGAGAGGAG TCGTTGGACCACAGGGTGCTCGTGGTTTCCCCGGGACTCCTGGACTTCCTGGCTTCAAAGGCGTGAAAGG ACACAGTGGTATGGATGGATTAAAGGGACAGCCTGGTGCACAGGGTGTGAAGGGTGAACCCGGTGCCCCT GGAGAGAATGGAACTCCAGGTCAAGCAGGAGCCCGAGGGCTTCCTGGTGAGAGAGGGCGTGTCGGAGCTC CTGGTCCAGCTGGTGCCCGAGGCAGTGATGGAAGCGTTGGCCCCGTCGGCCCTGCTGGTCCCATTGGGTC TGCTGGACCCCCTGGTTTCCCAGGTGCTCCTGGTCCCAAGGGTGAACTTGGGCCCGTTGGTAACCCTGGC CCTGCTGGTCCTGCTGGTCCCCGTGGTGAAGTGGGTCTTCCAGGTCTTTCTGGCCCTGTTGGACCTCCTG GCAACCCTGGAACAAATGGGCTCACTGGCGCCAAGGGTGCTACTGGACTCCCTGGTGTCGCTGGGGCTCC CGGTCTCCCTGGCCCCCGTGGTATTCCTGGCCCCGCTGGTGCTGCTGGTGCTACAGGTGCCAGAGGACTT GTTGGTGAGCCTGGTCCTGCTGGCTCCAAAGGAGAATCCGGTAACAAGGGTGAGCCTGGCTCTGTTGGAG CCCAAGGTCCTCCTGGTCCCAGCGGTGAAGAAGGAAAGAGAGGGTCTCCCGGAGAAGCTGGATCTGCTGG CCCTGCAGGGCCTCCAGGGCTTAGAGGCAGCCCTGGTTCTCGAGGTCTTCCTGGAGCTGATGGCAGAGCT GGTGTAATGGGTCCTCCTGGCAATCGTGGTTCAACCGGCCCTGCTGGAATCCGAGGTCCTAATGGAGATG CCGGTCGACCTGGGGAACCTGGTCTCATGGGACCCAGAGGTCTTCCTGGGTCTCCTGGAAATGTTGGCCC ATCTGGTAAAGAAGGCCCTGTGGGTCTCCCTGGCATTGATGGCAGACCTGGCCCAATCGGCCCAGCTGGA CCAAGAGGTGAAGCTGGCAACATTGGATTCCCTGGACCCAAAGGCCCCTCTGGTGATCCTGGCAAACCTG GTGAGAGAGGACACCCCGGTCTTGCTGGTGCTCGGGGAGCTCCAGGACCCGATGGCAACAATGGAGCTCA GGGCCCCCCTGGACCCCAGGGTGTTCAAGGTGGCAAAGGTGAACAAGGCCCTGCTGGTCCTCCTGGCTTC CAGGGTCTCCCTGGTCCCTCAGGTACTACTGGAGAAGTTGGCAAGCCCGGAGAAAGGGGTCTTCCTGGTG AATTCGGTCTCCCTGGTCCTGCTGGTCCAAGAGGAGAACGTGGTACCCCGGGTGAGAGTGGAGCTGCTGG CCCTTCTGGTCCTATTGGAAGCCGAGGTCCCAGTGGAGCCCCAGGGCCTGATGGAAACAAGGGTGAAGCT GGTGCAGTCGGTGCTCCAGGCAGTGCTGGTGCCTCTGGGCCTGGTGGGCTTCCAGGAGAGAGGGGTGCTG CTGGCATACCTGGGGGCAAAGGAGAAAAGGGTGAAACTGGTCTCCGAGGTGACACTGGCAACACTGGTAG AGATGGTGCTCGTGGCATTCCTGGTGCTGTAGGTGCCCCTGGTCCTGCTGGGGCCTCAGGTGACCGGGGT GAAGCTGGTGCTGCCGGTCCTTCTGGCCCAGCTGGTCCTCGGGGTAGCCCTGGTGAACGTGGTGAAGTTG GCCCTGCTGGCCCCAATGGATTTGCTGGTCCTGCTGGTGCTGCTGGCCAACCCGGTGCTAAAGGAGAGAA GGGAACCAAAGGGCCTAAGGGTGAAAATGGCATTGTTGGTCCAACCGGGTCTGTTGGAGCTGCTGGCCCA TCTGGTCCTAATGGCCCACCTGGTCCTGTTGGAAGTCGTGGTGATGGAGGGCCCCCTGGTATGACTGGCT TCCCTGGTGCTGCTGGACGGACTGGTCCCCCCGGACCCAGTGGTATTGCTGGCCCCCCTGGCCCCCCTGG TGCTGCTGGAAAGGAAGGGATTCGTGGTCCCCGTGGCGACCAAGGTCCAGTTGGCCGAACTGGAGAAACA GGTGCATCTGGACCCCCTGGATTTGTTGGTGAAAAGGGTCCCTCTGGAGAACCTGGTACCGCAGGAGCCC CTGGTACCGCAGGTCCTCAGGGTCTTCTTGGTGCTCCCGGTATTCTGGGTCTCCCTGGCTCTAGAGGTGA ACGTGGTCTGCCAGGTATTGCTGGTGCTCTGGGTGAACCTGGTCCTCTGGGCATCTCAGGCCCTCCTGGT GCCCGTGGTCCCCCTGGTGCTGTGGGCAGCCCTGGAGTTAATGGTGCCCCTGGTGAAGCTGGTCGTGATG GTAACCCTGGCAGCGATGGTCCCCCAGGTCGTGATGGTCAGCCTGGACACAAGGGAGAGCGTGGTTACCC TGGCAGCATTGGTCCGACTGGTGCTGCAGGTGCCCCTGGTCCTCACGGTTCTGTGGGTCCTGCTGGGAAA CATGGAAACCGAGGTGAACCTGGTCCTGCCGGTTCTGTTGGTCCTGTTGGTGCTGTTGGTCCAAGAGGTC CTAGTGGTCCTCAAGGCATCCGAGGTGACAAAGGAGAACCTGGTGATAAAGGGCACAGGGGTCTTCCTGG CTTAAAAGGATACAGTGGATTGCAGGGTCTTCCTGGTCTTGCTGGCCTACATGGTGACCAAGGAGCTCCT GGTCCTGTGGGTCCTGCTGGTCCCAGGGGTCCTGCTGGGCCTTCTGGACCCGTTGGCAAAGATGGTAGAT CTGGGCAACCTGGCCCAGTCGGGCCTGCTGGTGTTCGTGGTTCTCAGGGTAGCCAAGGTCCCGCTGGTCC CCCTGGTCCCCCTGGACCTCCTGGTCCTCCTGGTGTCAGCGGAGGTGGCTATGACTTTGGTTTTGAAGGA GACTTCTACAGGGCTGACCAGCCTCGCTCCCAGCCTTCACTCAGACCCAAGGACTATGAAGTTGATGCAA CTCTGAAATCTCTCAATAACCAGATTGAGACCCTTCTCACTCCTGAAGGCTCTAGAAAGAACCCTGCTCG CACGTGCCGGGACTTAAGACTCAGCCACCCAGAGTGGAACAGCGATTACTACTGGATTGACCCTAACCAA GGATGTACTATGGATGCCATCAAAGTGTACTGTGATTTCTCTACTGGTGAAACCTGCATCCAGGCCCAAC CTGTAAACACCCCAGCGAAGAACTCATACAGCCGCGCCCAGGCCAACAAGCATGTCTGGTTAGGAGAGAC TATCAATGGTGGCAGCCAGTTTGAATACAACGTAGAAGGGGTGTCCTCCAAGGAAATGGCAACTCAGCTC GCCTTCATGCGCCTGCTAGCCAACCGTGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGT ACCTGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTGAACTTGT TGCTGAGGGCAACAGCAGGTTCACCTACTCTGTCCTAGTCGATGGCTGCTCCAAAAAGACAAATGAATGG GGCAAGACAATCATTGAATACAAAACAAATAAGCCATCTCGCCTGCCGTTCCTTGACATTGCACCTCTGG ACATTGGTGGTGCTGACCAAGAATTCCGTGTGGAGGTTGGCCCCGTCTGTTTCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225252 representing NM_007743
Red=Cloning site Green=Tags(s) MLSFVDTRTLLLLAVTSCLATCQYLQSGSVRKGPTGDRGPRGQRGPAGPRGRDGVDGPMGPPGPPGSPGP PGSPAPPGLTGNFAAQYSDKGVSSGPGPMGLMGPRGPPGAVGAPGPQGFQGPAGEPGEPGQTGPAGPRGP AGSPGKAGEDGHPGKPGRPGERGVVGPQGARGFPGTPGLPGFKGVKGHSGMDGLKGQPGAQGVKGEPGAP GENGTPGQAGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGELGPVGNPG PAGPAGPRGEVGLPGLSGPVGPPGNPGTNGLTGAKGATGLPGVAGAPGLPGPRGIPGPAGAAGATGARGL VGEPGPAGSKGESGNKGEPGSVGAQGPPGPSGEEGKRGSPGEAGSAGPAGPPGLRGSPGSRGLPGADGRA GVMGPPGNRGSTGPAGIRGPNGDAGRPGEPGLMGPRGLPGSPGNVGPSGKEGPVGLPGIDGRPGPIGPAG PRGEAGNIGFPGPKGPSGDPGKPGERGHPGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPAGPPGF QGLPGPSGTTGEVGKPGERGLPGEFGLPGPAGPRGERGTPGESGAAGPSGPIGSRGPSGAPGPDGNKGEA GAVGAPGSAGASGPGGLPGERGAAGIPGGKGEKGETGLRGDTGNTGRDGARGIPGAVGAPGPAGASGDRG EAGAAGPSGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGEKGTKGPKGENGIVGPTGSVGAAGP SGPNGPPGPVGSRGDGGPPGMTGFPGAAGRTGPPGPSGIAGPPGPPGAAGKEGIRGPRGDQGPVGRTGET GASGPPGFVGEKGPSGEPGTAGAPGTAGPQGLLGAPGILGLPGSRGERGLPGIAGALGEPGPLGISGPPG ARGPPGAVGSPGVNGAPGEAGRDGNPGSDGPPGRDGQPGHKGERGYPGSIGPTGAAGAPGPHGSVGPAGK HGNRGEPGPAGSVGPVGAVGPRGPSGPQGIRGDKGEPGDKGHRGLPGLKGYSGLQGLPGLAGLHGDQGAP GPVGPAGPRGPAGPSGPVGKDGRSGQPGPVGPAGVRGSQGSQGPAGPPGPPGPPGPPGVSGGGYDFGFEG DFYRADQPRSQPSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWNSDYYWIDPNQ GCTMDAIKVYCDFSTGETCIQAQPVNTPAKNSYSRAQANKHVWLGETINGGSQFEYNVEGVSSKEMATQL AFMRLLANRASQNITYHCKNSIAYLDEETGSLNKAVLLQGSNDVELVAEGNSRFTYSVLVDGCSKKTNEW GKTIIEYKTNKPSRLPFLDIAPLDIGGADQEFRVEVGPVCFK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007743 |
ORF Size | 4116 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007743.2 |
RefSeq Size | 5143 bp |
RefSeq ORF | 4119 bp |
Locus ID | 12843 |
UniProt ID | Q01149 |
Gene Summary | This gene encodes the alpha-2 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-1 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice harboring certain mutations in the encoded gene exhibit symptoms of moderate to severe forms of osteogenesis imperfecta. [provided by RefSeq, Dec 2015] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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αKlotho Mitigates Progression of AKI to CKD through Activation of Autophagy
,Shi, M;Flores, B;Gillings, N;Bian, A;Cho, HJ;Yan, S;Liu, Y;Levine, B;Moe, OW;Hu, MC;,
J. Am. Soc. Nephrol.
,PubMed ID 26701976
[COL1A2]
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