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Dlc1 (NM_001194940) Mouse Tagged ORF Clone
CAT＃: MG225227
- TrueORF®
Dlc1 (tGFP-tagged) - Mouse deleted in liver cancer 1 (Dlc1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001194940" in other vectors (4)
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CNY 13,300.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A730069N07Rik; Arhgap7; dlc-1; HP; STARD12 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225227 representing NM_001194940
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTAGCTATCAGAAAGAGGAGCTGGGAAGAGCACGTGACCCAGCAGACCGAACAGCCTTTTATTT CTGATGAGTATGACATAGCATGTCACCACGGACTGGCATCCGACAGCTTGGAGGGAAGGATGGAAAAAGA TGCGACCTTAAATGTGGACCATAAAGAGAAGTGTGCCTCTCTACCGGACCGCTGTTGTGGGTCAGAGACA AGAGACTTTCCTGGGAGGCCAATGGGTCACATTGCACAGGAGGTAGATGAAAGTGACAGCCAAGAAGGGG AAGAGCGGTTTCTATCGCTCGAGGCCAGCACAGAAACACTAGTGCACATTTCGGATGAGGATACTGATTC TGATCTAAACCTTATCAATGACTCACAGATTTTAACTCCGCAAAGACATGAAAGAGACAGCCAAGATAGT GTCAAAGGAGCAGGCTCCTTCATGAAGACACTGTCCACCATGCAAAGTAGCCAAGATTCTCATAACACCT GGAGGATGGCTGGTAAGGCTGATATGTCTCCAGCAGAAGGAAGTGGAGAAAGGAAAGATGTTGAGGCTAT AAATAAAAGCCTGGGGCTTTGTAAGAATACGAGTGCAGATGAAACAAAAGATGTGCCCATAGGAAATACA TTCCAGTCTTTGAATACGGAAGATGACGTCACTCCTAAGACACTACTACTTGATTCTTCGGTACTCATAG AAGATGTCACACCTGAGACACAACTGCTTGATTCTGCAGTCATCACTCAACAATGCAGGAAATCTGACTT CCCTAAAGATGACCAGGAAAATAATCGGCACCATGTCTTAGAAGAAGACGTCTTAGCAGCTCCTTATGTA GACAGAGGGCTGCCATTATTAAAAGCAGATTGCAGAAGCAGCCTTCTTCAGCCTCCTTCCTGCCCTGGTG GGATGTCAGCAGAAAATGACCTGGAGAAGAGTGGATTCTCAGAATATCAAAACAAAAGCACTCTCGGGGT CAGCAGGGAAGATGGCATGCAGTGTTTACACTTAAGGGGCCCCGTGACCACCCAGGAGACTGTAGACAAT CAAGTCAGACTGCGCAAGAGAAAGGAAACAAGGGAAGATCGAGACAGGACACGGCTGGACTCCATGGTGC TGCTCATTATGAAACTGGACCAACTAGACCAAGACATAGAGAATGCTCTCAGCACTGCCTCCTCTCCATC CAGCACCCCAACGAACCTGCGGCGGCATGTCCCTGATCTGGAATCAGGATCTGAAAGCAGAACAGACAGT ATGGCAGTAAATCAGACACAGGTGAATCTGTCTTCAGAGTCCACCGATCCATCATCCACTCCAGGGAGCA ATTCTGGAACCAAACCCAAAGCCATGGCTGCTCCGTGTGTTGGAGAAAAGGAAATGGCTGAAATTGAAGC CAAGGAAGCTTGCGACTGGCTGAGAGTGACTGGTTTCCCCCAGTATGCACAGCTGTATGAAGATCTACTG TTCCCTATTGATATTGCGCTGGTCAAGAGAGAACATGACTTCTTGGACAGAGACGCCATCGAGGCTCTCT GCAGGCGCTTAAATACTCTAAACAAGTGTGCAGTCATGAAGCTGGAGATCAGTCCTCACCGGAAGCGAAG TGAGGATTCGGATGAAGATGAGCCTTGCGCCATAAGCGGCAAGTGGACTTTCCAGAGGGACAGCAAGCGG TGGTCCCGCCTTGAAGAGTTTGACGTCTTTTCCCCAAAGCAGGATCCAATCCCTGGGTCCCCAGACAACT CTCGTTTGCAAAGCGCCACAAGCCACGAAAGCATGCTGACAGACCTCAGCGAGCACCAGGAGGTGGCCTC TGTCCGAAGCCTCAGCAGCACCAGCAGCAGCGTCCCCACCCACGCAGCCCACAGTGGAGATGCCACTACG CCCCGAACCAATTCCGTCATCAGCGTCTGCTCCTCCGGACACTTTGTAGGCAATGATGACTCTTTTTCCA GCCTGCCGTCTCCCAAGGAACTGTCCAGCTTCAGTTTTAGCATGAAAGGCCACCACGAGAAGAACACCAA GTCGAAGACGCGGAGCCTGCTCAAACGCATGGAGAGCCTGAAGCTCAAGGGCTCCCACCACAGCAAGCAC AAGGCGCCTTCCAAGCTGGGGTTGATCATCAGTGCTCCCATTCTGCAGGAGGGTATGGATGAGGCAAAGC TGAAGCAGCTGAACTGTGTGGAGATCTCAGCCCTCAATGGCAACCACATCAACGTGCCCATGGTACGGAA AAGGAGCGTGTCTAACTCCACCCAGACCAGCAGCAGCAGCAGCCAATCAGAGACCAGCAGCGCGGTCAGC ACACCCAGCCCGGTCACCAGGACCCGGAGCCTCAGCACCTGTAACAAGCGGGTGGGCATGTATCTAGAGG GCTTCGACCCATTCAGTCAGTCCACCTTGAACAACGTGACGGAGCAGAACTATAAAAACCGTGAGAGCTA CCCAGAGGACACGGTGTTCTACATTCCCGAAGATCACAAGCCCGGCACCTTCCCTAAGGCCCTCTCCCAT GGCAGTTTCTGTCCCTCGGGAAACAGTTCTGTGAACTGGAGGACCGGAAGCTTCCATGGCCCTGGCCATC TCAGCCTACGGAGAGAAAACAGCCATGACAGTCCTAAGGAGCTGAAGAGACGCAATTCTTCCAGCTCTCT GAGCAGCCGCCTGAGCATCTATGATAACGTACCGGGTTCTATCCTGTACTCCAGCTCGGGAGAACTGGCC GACCTGGAGAATGAGGACATCTTCCCTGAGCTGGATGACATTCTCTACCACGTGAAGGGGATGCAGCGGA TAGTCAACCAGTGGTCCGAGAAGTTTTCCGACGAGGGAGACTCGGACTCAGCCCTGGACTCTGTCTCTCC TTGCCCGTCATCTCCAAAACAGATACACCTGGATGTGGACCATGACCGAAGGACACCCAGTGACCTGGAC AGCACAGGCAACTCCCTCAATGAGCCCGAAGAGCCCACTGATATCCCGGAAAGAAGAGACTCAGGGGTGG GGGCTTCCCTGACCAGGTGCAATAGGCACAGACTGAGGTGGCACAGCTTCCAGAGCTCCCACCGGCCGAG CCTCAACTCCGTGTCACTGCAGATTAACTGCCAGTCTGTGGCCCAGATGAACCTGCTGCAGAAATACTCG CTCCTGAAATTGACGGCCCTGCTGGAAAAGTACACGCCTTCCAACAAGCACGGCTTCAGCTGGGCTGTGC CCAAGTTCATGAAAAGGATCAAGGTTCCAGACTACAAGGACCGGAGTGTGTTTGGGGTCCCACTGACTGT GAACGTGCAGCGCTCAGGACAGCCCCTGCCCCAGAGCATCCAGCAGGCCATGCGCTACCTCCGTAACCAC TGTCTGGACCAGGTCGGGCTCTTCAGGAAGTCAGGTGTCAAATCCCGGATCCAGGCTCTACGCCAGATGA ATGAAAGCGCTGAAGATAATGTCAACTATGAAGGCCAGTCTGCTTATGATGTGGCAGACATGTTAAAGCA ATATTTTCGAGATCTTCCTGAGCCCCTCATGACGAACAAACTCTCCGAAACCTTCCTGCAGATCTACCAG TATGTTCCCAAGGACCAGCGCCTCCAAGCCATCAAGGCGGCCATTATGCTCCTGCCCGACGAGAACCGGG AGGTTCTGCAGACACTTCTCTATTTCCTGAGCGATGTCACAGCGGCTGTGAAAGAAAACCAGATGACTCC CACCAACCTGGCTGTGTGCCTAGCTCCGTCCCTCTTCCACCTCAACACCCTGAAGCGAGAGAATTCTTCT CCAAGGGTAATGCAAAGAAAACAGAGTTTGGGCAAACCAGACCAGAAAGACCTGAATGAAAACCTAGCGG CGACTCAAGGGCTGGCCCACATGATTGCTGAGTGCAAGAAGCTCTTCCAGGTCCCTGAGGAAATGAGCCG GTGCCGTAACTCCTACACTGAACAAGAGCTGAAGCCCCTTACCCTGGAGGCCTTGGGACACCTGAATAGT GACCAGCCTGCTGACTACAGACACTTCCTCCAGGACTGTGTGGATGGCCTGTTTAAGGAGGTCAAAGAGA AGTTCAAAGGCTGGGTCAGCTACCCCACCTCCGAACAGGCTGAGCTGTCCTATAAGAAGGTCAGCGAAGG ACCCCCGTTAAGGCTTTGGAGGTCAACTATCGAAGTCCCCGCTGCACCCGAGGAGATCTTAAAGCGCCTT CTGAAGGAGCAACACCTCTGGGATGTGGACCTGCTGGACTCCAAGGTGATTGAAATCCTGGACAGCCAGA CTGAAATCTACCAATACGTCCAAAACAGCATGGCGCCCCACCCTGCGCGGGACTACGTCGTATTGAGGAC CTGGAGGACTAACTTACCCAGGGGGGCCTGTGCTCTCTTACTCACCTCTGTGGATCACGACCGGGCCCCC GTGGCGGGGGTGAGGGTTAATGTGCTCCTGTCCAGATATTTGATTGAACCCTGCGGGTCAGGAAAATCCA AGCTTACCTACATGTGCAGAGCTGATTTAAGGGGCCACATGCCGGAGTGGTACTCAAAATCTTTTGGACA TTTGTGTGCAGCCGAAGTGGTAAAGATCCGAGACTCCTTCAGTAATCAGAACACTGAAAGCAAGGACACG AGATCTAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225227 representing NM_001194940
Red=Cloning site Green=Tags(s) MSVAIRKRSWEEHVTQQTEQPFISDEYDIACHHGLASDSLEGRMEKDATLNVDHKEKCASLPDRCCGSET RDFPGRPMGHIAQEVDESDSQEGEERFLSLEASTETLVHISDEDTDSDLNLINDSQILTPQRHERDSQDS VKGAGSFMKTLSTMQSSQDSHNTWRMAGKADMSPAEGSGERKDVEAINKSLGLCKNTSADETKDVPIGNT FQSLNTEDDVTPKTLLLDSSVLIEDVTPETQLLDSAVITQQCRKSDFPKDDQENNRHHVLEEDVLAAPYV DRGLPLLKADCRSSLLQPPSCPGGMSAENDLEKSGFSEYQNKSTLGVSREDGMQCLHLRGPVTTQETVDN QVRLRKRKETREDRDRTRLDSMVLLIMKLDQLDQDIENALSTASSPSSTPTNLRRHVPDLESGSESRTDS MAVNQTQVNLSSESTDPSSTPGSNSGTKPKAMAAPCVGEKEMAEIEAKEACDWLRVTGFPQYAQLYEDLL FPIDIALVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKR WSRLEEFDVFSPKQDPIPGSPDNSRLQSATSHESMLTDLSEHQEVASVRSLSSTSSSVPTHAAHSGDATT PRTNSVISVCSSGHFVGNDDSFSSLPSPKELSSFSFSMKGHHEKNTKSKTRSLLKRMESLKLKGSHHSKH KAPSKLGLIISAPILQEGMDEAKLKQLNCVEISALNGNHINVPMVRKRSVSNSTQTSSSSSQSETSSAVS TPSPVTRTRSLSTCNKRVGMYLEGFDPFSQSTLNNVTEQNYKNRESYPEDTVFYIPEDHKPGTFPKALSH GSFCPSGNSSVNWRTGSFHGPGHLSLRRENSHDSPKELKRRNSSSSLSSRLSIYDNVPGSILYSSSGELA DLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDHDRRTPSDLD STGNSLNEPEEPTDIPERRDSGVGASLTRCNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYS LLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRSVFGVPLTVNVQRSGQPLPQSIQQAMRYLRNH CLDQVGLFRKSGVKSRIQALRQMNESAEDNVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQ YVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSS PRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLNS DQPADYRHFLQDCVDGLFKEVKEKFKGWVSYPTSEQAELSYKKVSEGPPLRLWRSTIEVPAAPEEILKRL LKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPRGACALLLTSVDHDRAP VAGVRVNVLLSRYLIEPCGSGKSKLTYMCRADLRGHMPEWYSKSFGHLCAAEVVKIRDSFSNQNTESKDT RSR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001194940 |
| ORF Size | 4629 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001194940.2, NP_001181869.1 |
| RefSeq Size | 7189 bp |
| RefSeq ORF | 4632 bp |
| Locus ID | 50768 |
| Gene Summary | Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224663 | Dlc1 (untagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 1, (10ug) |
CNY 10,568.00 |
|
| MR225227 | Dlc1 (Myc-DDK-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 1 |
CNY 10,560.00 |
|
| MR225227L3 | Lenti ORF clone of Dlc1 (Myc-DDK-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 1 |
CNY 13,970.00 |
|
| MR225227L4 | Lenti ORF clone of Dlc1 (mGFP-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 1 |
CNY 13,970.00 |
