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Nasp (NM_016777) Mouse Tagged ORF Clone
CAT＃: MG225025
- TrueORF®
Nasp (tGFP-tagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_016777" in other vectors (3)
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CNY 8,460.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5033430J04Rik; AI131596; AI317140; D4Ertd767e; Epcs32; Nasp-T |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225025 representing NM_016777
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGAGTCTACAGCCGCTGCTGCCATCGCCGCGGAGCTGGTTTCCGCTGACAAAATTGAAGATG CTCCTGCTCCTTCTACCTCGGCAGATAAAATGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTACT GGGATTAGGACAGAAACATCTGGTGATGGGTGACATTCCAGCAGCTGTTAATGCATTCCAGGAAGCAGCT AGTCTTTTAGGTAAGAAGTATGGAGAAACGGCTAATGAGTGTGGAGAAGCCTTCTTTTTTTATGGGAAGT CACTTCTGGAGTTAGCCAGAATGGAGAATGGAGTGTTGGGAAATGCCTTAGAAGGAGTGCATGTAGAAGA GGAAGAAGGAGAAAAAACAGAAGATGAATCTTTGGTAGAAAATAATGATAATGTAGATGAGGAAGCAAGG GAAGAGTTGAGAGAACAGGTTTATGACGCCATGGGAGAAAAAGAAGCCAAAAAAGCAGAAGGCAAGTCTC TGACAAAGCCTGAAACTGATAAAGAACAGGAAAGTGAAGTGGAGAAGGGTGGAAGAGAAGACATGGATAT AAGTGAGCCTGAAGAGAAGCTCCAGGAAACAGTTGAACCGACTTCAAAGCAGTTAACTGAATCCTCTGAA GAGGCAAAAGAAGCAGCAATACCAGGACTGAATGAAGATGAGGTCGCTTCTGGGAAGACAGAGCAGGAAT CATTGTGTACTGAGAAAGGAAAATCAATTTCAGGAGCTTATGTTCAAAATAAAGAATTCAGAGAAACAGT GGAGGAGGGAGAGGAAATAATAAGCTTAGAGAAAAAGCCAAAAGAAACTTCAGAAGATCAGCCTATCAGG GCTGCAGAAAAGCAGGGCACTTTAATGAAGGTGGTAGAAATAGAAGCTGAAATAGACCCTCAAGTCAAGT CAGCGGATGTGGGAGGGGAGGAGCCAAAAGATCAGGTAGCTACCTCTGAGAGTGAACTAGGTAAGGCTGT TCTTATGGAACTGTCAGGGCAAGATGTTGAAGCATCACCAGTCGTGGCTGCAGAGGCCGGAGCTGAAGTC TCTGAGAAGCCAGGGCAGGAGATTACAGTTATTCCCAACAATGGTCCAGTTGTTGGACAATCAACTGTAG GAGATCAGACTCCTAGTGAACCACAGACTTCTGCAGAAAGACTGACAGAAACTAAAGATGGCTCAAGTGT AGAGGAGGTCAAGGCAGAGCTGGTTCCTGAACAGGAGGAAGCTATGCTACCTGTAGAAGAGTCTGAGGCA GCTGGAGATGGGGTTGAGACCAAGGTAGCCCAGAGGGCCACGGAGAAAGCACCTGAAGACAAATTTAAGA TAGCTGCTAATGAAGAGACACAAGAGAGAGACGAACAGATGAAAGAGGGTGAAGAAACTGAAGGCTCAGA GGAAGAGGACAGAGAAAATGACAAGGCTGAAGAAACACCAAATGAATCAGTTCTTGAAAAAAAGTCTCTT CAAGAAAATGAAGAGGAGGAGATAGGGAATTTGGAACTTGCCTGGGATATGCTGGATTTAGCCAAGATCA TTTTTAAGAGGCAAGAAACAAAAGAAGCCCAGCTTTATGCTGCACAAGCCCATCTTAAACTTGGAGAAGT TAGTGTTGAATCTGAGAATTATATCCAAGCTGTGGAGGAGTTTCAGGCTTGCCTAAGCCTGCAAGAACAA TATTTGGAAGCTCACGATCGTCTCCTTGCAGAGACTCACTACCAATTAGGTTTGGCCTATGGTTACAACT CTCAGTATGATGAAGCAGTAGCACAGTTTGGCAAATCTATCGATGTCATTGAAAAGAGAATGGCTGTACT CCATGAGCAGATGAAGGAGGCCGAAGGATCGTTTACTGAATATGAGAAAGAAATTGAGGAGCTGAAAGAA CTGCTACCTGAAATTCGAGAGAAGATAGAAGACGCAAAGGAATCTCAGCGGAGTGGGAATGTAGCCGAAC TGGCACTGAAAGCTACTCTGGTGGAGAGCTCTACTTCCGGTTTCACTCCTAGTGGAGCTGGTGCCTCAGT ATCCATGATTGCCAGTAGAAAACCAACAGATGGCGCTTCCTCATCAAATTGTGTGACTGATATTTCCCAT CTTGTCAGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAGGATGATGCAAAGAAAGCTAAACAAG AGCCAGAGGTGAATGGAGGCAGTGGGGATGCTGTCTCCAGTGGAAAGGAAGTTTCAGAAAACATGGAGGC CGAGGCTGAGAATCAGGCTGAGAGCCAAACAGCAGAGGGGACCGTGGAGTCTGCAGCTACAATTAAAAGC ACTGCATGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225025 representing NM_016777
Red=Cloning site Green=Tags(s) MATESTAAAAIAAELVSADKIEDAPAPSTSADKMESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAA SLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNVDEEAR EELREQVYDAMGEKEAKKAEGKSLTKPETDKEQESEVEKGGREDMDISEPEEKLQETVEPTSKQLTESSE EAKEAAIPGLNEDEVASGKTEQESLCTEKGKSISGAYVQNKEFRETVEEGEEIISLEKKPKETSEDQPIR AAEKQGTLMKVVEIEAEIDPQVKSADVGGEEPKDQVATSESELGKAVLMELSGQDVEASPVVAAEAGAEV SEKPGQEITVIPNNGPVVGQSTVGDQTPSEPQTSAERLTETKDGSSVEEVKAELVPEQEEAMLPVEESEA AGDGVETKVAQRATEKAPEDKFKIAANEETQERDEQMKEGEETEGSEEEDRENDKAEETPNESVLEKKSL QENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYIQAVEEFQACLSLQEQ YLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFGKSIDVIEKRMAVLHEQMKEAEGSFTEYEKEIEELKE LLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPSGAGASVSMIASRKPTDGASSSNCVTDISH LVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVSSGKEVSENMEAEAENQAESQTAEGTVESAATIKS TAC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016777 |
| ORF Size | 2319 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016777.3, NP_058057.3 |
| RefSeq Size | 3087 bp |
| RefSeq ORF | 2322 bp |
| Locus ID | 50927 |
| Gene Summary | Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and linker H1 histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR225025 | Nasp (Myc-DDK-tagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp), transcript variant 2 |
CNY 7,700.00 |
|
| MR225025L3 | Lenti ORF clone of Nasp (Myc-DDK-tagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp), transcript variant 2 |
CNY 9,600.00 |
|
| MR225025L4 | Lenti ORF clone of Nasp (mGFP-tagged) - Mouse nuclear autoantigenic sperm protein (histone-binding) (Nasp), transcript variant 2 |
CNY 9,600.00 |
