Use1 (NM_025917) Mouse Tagged ORF Clone
CAT#: MG224938
- TrueORF®
Use1 (tGFP-tagged) - Mouse unconventional SNARE in the ER 1 homolog (S. cerevisiae) (Use1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_025917" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2010315L10Rik; 5730403H22Rik; AV002165; D12; Ed2; p31; Q-snare |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224938 representing NM_025917
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGGCGGAAGGAGCCTATCATCGCCCGCTGGCGACGTCGCGGTTGGAGTTGAACCTGGTACGGC TGCTTTGTCGCTGTGAGTCGATGGCGGCAGAAAAACGAGAACCGGACGAGTGGCGACTGGAGAAGTATGT GGGTGCCCTGGAAGACATGCTGCAGGCCCTGAAAGTTCAAGCAAGTAAACCGGCCTCAGAAGTGATCAGC GAGTACTCCCGCAAAGTGGACTTTCTGAAGGGCATGCTGCAGGCCGAGAAGTTGACCTCCTCCTCTGAAA AAGCTCTAGCTAACCAGTTCCTGGCTCCTGGCCGCGTGCCCACCACAGCCAAGGAGCGGGTGCCTGCCAC CAAGACTGTACATTTGCAGTCTAGGGCACGTTACACCAGTGAGATGCGGAGTGAGCTGTTGGGCATGGAA CCATCTGGAGAATGTGAGGTGGACATGAGGAAACGAGCTGCGAAGGGGTCCAGACCTGCAGATGAGAGGC AGTCAGCATCCGAGCTAGACCTCGTCCTCCAGAGGCACCAGGGCCTCCAGGAAAAGCTGGCTGAGGAGAT GCTAGGCCTGGCCCGCAGCCTCAAGACCAACACACTGGCTGCCCAGAGTGTCATCAAGAAGGACAATCAG ACCCTGTCACATTCACTCAAGATGGCAGACCAGAACCTGGAGAAGCTGAAGCTGGAGTCGGAGAGATTGG AGCAGCATGCACAGAAGTCAGTGAACTGGCTGCTGTGGGCCATGCTCATTGTCGTGTGCTTCGTGTTCAT CAGCATGATCCTGTTTATCCGAATCATGCCCAGGCTGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224938 representing NM_025917
Red=Cloning site Green=Tags(s) MAQAEGAYHRPLATSRLELNLVRLLCRCESMAAEKREPDEWRLEKYVGALEDMLQALKVQASKPASEVIS EYSRKVDFLKGMLQAEKLTSSSEKALANQFLAPGRVPTTAKERVPATKTVHLQSRARYTSEMRSELLGME PSGECEVDMRKRAAKGSRPADERQSASELDLVLQRHQGLQEKLAEEMLGLARSLKTNTLAAQSVIKKDNQ TLSHSLKMADQNLEKLKLESERLEQHAQKSVNWLLWAMLIVVCFVFISMILFIRIMPRLK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025917 |
ORF Size | 810 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025917.4, NP_080193.1 |
RefSeq Size | 874 bp |
RefSeq ORF | 813 bp |
Locus ID | 67023 |
UniProt ID | Q9CQ56 |
Gene Summary | SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG203593 | Use1 (tGFP-tagged) - Mouse RIKEN cDNA 2010315L10 gene (2010315L10Rik), transcript variant 1 |
CNY 2,850.00 |
|
MR224938 | Use1 (Myc-DDK-tagged) - Mouse unconventional SNARE in the ER 1 homolog (S. cerevisiae) (Use1), transcript variant 1 |
CNY 2,400.00 |
|
MR224938L3 | Lenti ORF clone of Use1 (Myc-DDK-tagged) - Mouse unconventional SNARE in the ER 1 homolog (S. cerevisiae) (Use1), transcript variant 1 |
CNY 4,750.00 |
|
MR224938L4 | Lenti ORF clone of Use1 (mGFP-tagged) - Mouse unconventional SNARE in the ER 1 homolog (S. cerevisiae) (Use1), transcript variant 1 |
CNY 4,750.00 |