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Phf23 (NM_030064) Mouse Tagged ORF Clone
CAT＃: MG224822
- TrueORF®
Phf23 (tGFP-tagged) - Mouse PHD finger protein 23 (Phf23), (10ug)
ORF Plasmid: DDK
"NM_030064" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | JUNE-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224822 representing NM_030064
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGAAGCCATGGCGGAGCCCAGTCCCGAAGATCCGCCTCCGACTCTTAAACCAGAGACTCAGCCAC CGGAGAAAAGGCGGAGAACCATTGAGGATTTTAACAAATTCTGCAGTTTTGTCTTGGCCTATGCTGGTTA CATCCCCCCTAGCAAAGAGGAAAGCGATTGGCCAGCCTCTGGCTCTAGCTCTCCATTGCGAGGTGAGAGT GCAGCAGACAGTGATGGCTGGGACTCGGCCCCGTCGGACCTTCGAACTATCCAGACTTTTGTTAAGAAAG CAAAGTCATCAAAGAGAAGGGCAGTTCAATCAGGTCCTACCCAGCCAGGACCCCCAAGGTCCACTTTTTC TCGTCTGCAGGCTCCTGACAGTGCTACTCTGCTTGAGAAGATGAAGCTCAAGGACTCTCTTTTTGATCTG GATGGGCCCAAAGTGGCATCTCCACTCTCTCCCACATCATTGACGCATACTTCCCGGCCCCCCGCTGCTC TCGCCCCAGTGCCGCTGTCCCAGGGGGACCTCTCCCAGCCTCGAAAGAAGGACCGAAAGAATAGAAAGTT GGGACCAGGAGGTGGGGCTGGCTTTGGGGTGCTTCGGAGACCTCGACCAGCTCCTGGGGATGGGGAAAAG CGGTCTCGAATCAAGAAGAGCAAGAAGCGGAAGTTGAAAAAGGCAGATCGGGGAGATAGACTCCCACCTC CTGGCCCTCCTAGGGCTCCTCCCAGTGATACAGACTCTGAAGAGGAGGAGGAAGAGGAAGAAGAGGAAGA TGATGAGGAAGAGATGACAGTGGGGGGTGGAGTCCCAGCCCCTGTGCTCCCAACACCCCCTGAGGCCCCC AGGCCCCCTGTTACAGTGCACTCTGAAGGTGCTCCCCCTACTGACAGTGAAGGCAAAGATGTGGGCAGCA CAGAAACAAGCCAAGATGGAGATGCTAGCTCCAGTGAAGGCGAGATGCGGGTCATGGATGAGGACATCAT GGTAGAATCAGGTGACGACTCCTGGGATCTGATCACGTGTTACTGCAGAAAGCCCTTTGCGGGGCGGCCC ATGATTGAGTGCAGCCTCTGTGGGACGTGGATCCACCTCTCCTGTGCTAAGATTAAGAAGACCAACGTTC CCGACTTCTTTTATTGCCAGAAATGCAAGGAACTTCGGCCAGAGGCCCGGCGGTTAGGGGGTCTCCCCAA ATCTGGAGAACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224822 representing NM_030064
Red=Cloning site Green=Tags(s) MLEAMAEPSPEDPPPTLKPETQPPEKRRRTIEDFNKFCSFVLAYAGYIPPSKEESDWPASGSSSPLRGES AADSDGWDSAPSDLRTIQTFVKKAKSSKRRAVQSGPTQPGPPRSTFSRLQAPDSATLLEKMKLKDSLFDL DGPKVASPLSPTSLTHTSRPPAALAPVPLSQGDLSQPRKKDRKNRKLGPGGGAGFGVLRRPRPAPGDGEK RSRIKKSKKRKLKKADRGDRLPPPGPPRAPPSDTDSEEEEEEEEEEDDEEEMTVGGGVPAPVLPTPPEAP RPPVTVHSEGAPPTDSEGKDVGSTETSQDGDASSSEGEMRVMDEDIMVESGDDSWDLITCYCRKPFAGRP MIECSLCGTWIHLSCAKIKKTNVPDFFYCQKCKELRPEARRLGGLPKSGEP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030064 |
| ORF Size | 1203 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030064.4, NP_084340.2 |
| RefSeq Size | 2014 bp |
| RefSeq ORF | 1206 bp |
| Locus ID | 78246 |
| UniProt ID | Q8BSN5 |
| Gene Summary | Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211865 | Phf23 (untagged) - Mouse PHD finger protein 23 (Phf23), (10ug) |
CNY 3,990.00 |
|
| MR224822 | Phf23 (Myc-DDK-tagged) - Mouse PHD finger protein 23 (Phf23) |
CNY 3,990.00 |
|
| MR224822L3 | Lenti ORF clone of Phf23 (Myc-DDK-tagged) - Mouse PHD finger protein 23 (Phf23) |
CNY 5,890.00 |
|
| MR224822L4 | Lenti ORF clone of Phf23 (mGFP-tagged) - Mouse PHD finger protein 23 (Phf23) |
CNY 5,890.00 |
