Lgr6 (NM_001033409) Mouse Tagged ORF Clone
CAT#: MG224646
- TrueORF®
Lgr6 (tGFP-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6), (10ug)
ORF Plasmid: DDK
"NM_001033409" in other vectors (4)
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CNY 7,890.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | A530037C04Rik; D830026M09 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224646 representing NM_001033409
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACAGCCCGCCTGGGCTCCTGGCGCTGTGGCTTTGCGCTGTGCTGTGCGCATCGGCGCGCGGGGGCA GCGACCCCCAGCCTGGCCCGGGGCGTCCCGCCTGCCCGGCTCCCTGCCACTGCCAGGAGGACGGCATCAT GCTGTCCGCTGACTGCTCCGAGCTCGGGCTCTCAGTGGTGCCTGCGGACCTGGACCCCCTGACGGCTTAC CTAGACCTCAGTATGAACAACCTCACGGAGCTTCAGCCGGGTCTCTTCCACCACCTGCGCTTCCTGGAGG AGCTGCGGCTCTCAGGGAACCACCTCTCACACATCCCGGGACAGGCATTCTCCGGCCTCCACAGCCTCAA AATTCTAATGCTGCAGAGCAACCAGCTCCGTGGGATCCCAGCAGAGGCACTATGGGAGCTGCCCAGCCTG CAGTCGCTGCGCCTAGATGCTAATCTCATCTCCCTGGTCCCTGAGAGAAGCTTTGAGGGGCTCTCCTCCC TCCGCCACCTCTGGCTGGATGACAATGCACTCACTGAGATCCCCGTCAGAGCTCTCAACAACCTTCCTGC CCTACAGGCCATGACCTTGGCTCTCAACCATATCCGCCACATCCCTGACTATGCCTTCCAGAACCTCACC AGTCTTGTGGTGCTGCATCTACATAACAACCGCATCCAGCATGTGGGGACCCACAGCTTCGAGGGGCTGC ACAATCTGGAGACACTAGACCTGAACTATAATGAGCTGCAGGAGTTCCCCTTGGCTATCCGGACCCTGGG CAGGCTGCAGGAATTGGGTTTCCATAACAACAACATCAAGGCTATCCCAGAGAAAGCCTTCATGGGCAAC CCTCTCCTGCAGACAATACATTTTTATGACAACCCAATCCAGTTTGTGGGAAGGTCAGCATTCCAGTACC TGTCTAAACTGCATACGCTATCTTTGAATGGTGCCACTGATATCCAAGAGTTCCCAGACCTCAAAGGCAC CACTAGCCTGGAGATCCTGACCCTGACCCGTGCGGGCATCAGACTGCTCCCACCGGGAGTGTGCCAACAG CTGCCTAGGCTCCGAATCCTGGAGCTGTCTCATAATCAGATCGAGGAGTTACCCAGCCTGCACAGATGTC AGAAGCTGGAGGAAATTGGCCTCCGACATAACAGGATCAAGGAAATTGGTGCAGATACCTTCAGCCAGCT GGGCTCCTTGCAAGCTTTAGACCTGAGTTGGAATGCCATCCGTGCCATCCACCCTGAGGCTTTCTCAACC CTTCGATCCTTGGTTAAGCTGGACCTGACTGACAACCAGCTGACCACACTGCCCCTGGCTGGGCTGGGAG GCCTGATGCACCTGAAGCTCAAAGGGAACTTGGCCCTGTCTCAGGCCTTCTCCAAGGACAGTTTCCCAAA ACTGAGGATCCTGGAGGTGCCCTACGCCTACCAGTGCTGTGCCTACGGCATCTGTGCCAGCTTCTTCAAG ACCTCTGGGCAGTGGCAGGCCGAGGACTTTCATCCAGAAGAAGAGGAGGCACCAAAGAGGCCCCTGGGTC TCCTTGCTGGACAAGCTGAGAACCACTATGACCTAGACCTGGATGAGCTCCAGATGGGGACAGAGGACTC AAAGCCAAACCCCAGTGTCCAGTGCAGCCCTGTTCCAGGCCCCTTCAAGCCCTGCGAGCACCTCTTTGAG AGCTGGGGCATCCGCCTTGCTGTGTGGGCCATCGTGTTACTCTCCGTACTCTGTAACGGGCTGGTGCTGC TGACAGTCTTTGCCAGCGGACCCAGCCCGCTGTCCCCCGTCAAGCTTGTGGTGGGTGCGATGGCAGGCGC CAACGCCCTGACGGGCATTTCCTGTGGTCTCCTGGCCTCGGTGGACGCCTTGACCTATGGTCAGTTCGCT GAGTATGGAGCCCGCTGGGAGAGCGGTCTGGGCTGCCAGGCTACGGGCTTCCTGGCTGTCCTGGGTTCAG AGGCGTCGGTGCTGCTGCTCACACTGGCGGCGGTGCAGTGCAGCATCTCTGTGACCTGCGTCCGAGCCTA CGGGAAGGCGCCGTCGCCTGGCAGCGTCCGCGCAGGCGCACTGGGATGCCTGGCGCTGGCCGGGCTGGCC GCAGCACTGCCGCTGGCCTCGGTGGGAGAGTATGGCGCCTCCCCACTCTGCCTGCCCTACGCCCCACCCG AGGGCCGGCCGGCCGCCCTGGGCTTCGCTGTAGCCCTGGTGATGATGAACTCGCTCTGCTTCCTGGTGGT GGCCGGCGCCTACATCAAGCTCTACTGTGACCTGCCACGGGGTGACTTTGAGGCCGTGTGGGACTGCGCC ATGGTGCGCCACGTGGCCTGGCTCATCTTTGCAGATGGCCTCCTCTACTGCCCCGTGGCCTTCCTCAGCT TTGCCTCTATGCTGGGCCTCTTCCCTGTCACCCCCGAGGCTGTCAAGTCAGTCCTTCTGGTGGTGCTGCC TCTGCCTGCCTGCCTCAACCCACTGCTCTACCTGCTCTTCAACCCTCACTTCCGGGATGACCTTCGGCGG CTCTGGCCAAGCCCTCGGTCCCCAGGGCCCCTAGCCTACGCTGCAGCCGGTGAGCTGGAGAAGAGCTCCT GCGACTCCACCCAAGCGCTGGTGGCTTTCTCAGATGTGGATCTTATTCTGGAAGCTTCTGAGGCTGGGCA GCCTCCTGGGCTAGAGACCTATGGCTTCCCTTCAGTGACCCTCATCTCCCGACATCAGCCGGGGGCCACC AGGCTGGAGGGAAACCATTTTATAGAGTCTGATGGAACCAAGTTTGGGAACCCACAACCTCCCATGAAGG GAGAACTGCTGCTGAAGGCAGAGGGAGCCACTTTGGCAGGCTGTGGCTCTTCCGTGGGTGGAGCCCTCTG GCCCTCTGGCTCTCTCTTTGCCTCTCACTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224646 representing NM_001033409
Red=Cloning site Green=Tags(s) MHSPPGLLALWLCAVLCASARGGSDPQPGPGRPACPAPCHCQEDGIMLSADCSELGLSVVPADLDPLTAY LDLSMNNLTELQPGLFHHLRFLEELRLSGNHLSHIPGQAFSGLHSLKILMLQSNQLRGIPAEALWELPSL QSLRLDANLISLVPERSFEGLSSLRHLWLDDNALTEIPVRALNNLPALQAMTLALNHIRHIPDYAFQNLT SLVVLHLHNNRIQHVGTHSFEGLHNLETLDLNYNELQEFPLAIRTLGRLQELGFHNNNIKAIPEKAFMGN PLLQTIHFYDNPIQFVGRSAFQYLSKLHTLSLNGATDIQEFPDLKGTTSLEILTLTRAGIRLLPPGVCQQ LPRLRILELSHNQIEELPSLHRCQKLEEIGLRHNRIKEIGADTFSQLGSLQALDLSWNAIRAIHPEAFST LRSLVKLDLTDNQLTTLPLAGLGGLMHLKLKGNLALSQAFSKDSFPKLRILEVPYAYQCCAYGICASFFK TSGQWQAEDFHPEEEEAPKRPLGLLAGQAENHYDLDLDELQMGTEDSKPNPSVQCSPVPGPFKPCEHLFE SWGIRLAVWAIVLLSVLCNGLVLLTVFASGPSPLSPVKLVVGAMAGANALTGISCGLLASVDALTYGQFA EYGARWESGLGCQATGFLAVLGSEASVLLLTLAAVQCSISVTCVRAYGKAPSPGSVRAGALGCLALAGLA AALPLASVGEYGASPLCLPYAPPEGRPAALGFAVALVMMNSLCFLVVAGAYIKLYCDLPRGDFEAVWDCA MVRHVAWLIFADGLLYCPVAFLSFASMLGLFPVTPEAVKSVLLVVLPLPACLNPLLYLLFNPHFRDDLRR LWPSPRSPGPLAYAAAGELEKSSCDSTQALVAFSDVDLILEASEAGQPPGLETYGFPSVTLISRHQPGAT RLEGNHFIESDGTKFGNPQPPMKGELLLKAEGATLAGCGSSVGGALWPSGSLFASHL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033409 |
ORF Size | 2901 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033409.3, NP_001028581.1 |
RefSeq Size | 3623 bp |
RefSeq ORF | 2904 bp |
Locus ID | 329252 |
UniProt ID | Q3UVD5 |
Gene Summary | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222887 | Lgr6 (untagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6), (10ug) |
CNY 7,088.00 |
|
MR224646 | Lgr6 (Myc-DDK-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6) |
CNY 7,088.00 |
|
MR224646L3 | Lenti ORF clone of Lgr6 (Myc-DDK-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6) |
CNY 9,030.00 |
|
MR224646L4 | Lenti ORF clone of Lgr6 (mGFP-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 6 (Lgr6) |
CNY 9,030.00 |