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Fbxo11 (NM_001081034) Mouse Tagged ORF Clone
CAT＃: MG224533
- TrueORF®
Fbxo11 (tGFP-tagged) - Mouse F-box protein 11 (Fbxo11), (10ug)
ORF Plasmid: DDK
"NM_001081034" in other vectors (4)
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CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C80048; Jf |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224533 representing NM_001081034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCCGTCCGAGCCGCCAACCGGAGACCCAGGCGAGTGTCGCGGCCGCGCCCGGTGCAACAGCAGC AGCAGCAGCCCCCGCAGCAGCCGCCGCCGCAGCCGCCTCAGCAACAGCCGCCGCCGCAGCCGCCCCAGCA GCCGCCGCAGCAGCAGCCCCCGCCGCCGCCGCAGCAGCAGCCCCCGCCGCCGCCCCCGCCGCCTCCGCCG CCGCCTCAGGATCGGAACAACGCCGGCGAGAGGGATGATGTTCCTGCAGATATGGTTGCAGAAGAATCAG GTCCTGGTGCACAAAATAGTCCATACCAACTTCGCAGAAAAACTCTTTTGCCAAAAAGAACAGCGTGTCC TACAAAGAGCAGTATGGAGGGTGCCTCTACTTCGACTACAGAAAACTTTGGTCATCGAGCAAAGCGTGCA AGAGTGTCTGGAAAGTCACAAGATCTTTCAGCAGCACCCGCTGAACAGTATCTTCAGGAGAAGCTGCCAG ATGAAGTAGTTTTAAAAATATTTTCTTATTTGCTGGAACAAGACCTTTGTAGAGCTGCTTGCGTGTGTAA ACGCTTCAGTGAGCTTGCTAATGATCCAATTTTGTGGAAACGATTATATATGGAAGTATTTGAATATACC CGGCCGATGATGCATCCTGAACCTGGTAAATTCTACCAGATTAATCCAGAAGAATATGAACATCCAAATC CATGGAAAGAAAGTTTTCAACAGTTGTATAAAGGTGCGCATGTAAAGCCAGGATTTGCGGAACATTTCTA TAGTAATCCTGCAAGATACAAAGGAAGAGAAAACATGTTGTATTATGATACTATTGAAGATGCCCTTGGA GGAGTACAAGAAGCACATTTTGATGGGCTTATCTTTGTTCATTCTGGAATATATACTGATGAATGGATAT ATATTGAATCTCCAATTACTATGATTGGTGCAGCACCTGGCAAGGTTGCAGACAAGGTCATCATTGAGAA CACTAGAGATTCGACCTTCGTGTTCATGGAAGGCTCTGAAGATGCTTATGTTGGATACATGACGATAAGA TTCAACCCTGATGACAAATCTGCTCAGCATCACAACGCACACCACTGCTTAGAGATCACAGTCAACTGCA GCCCTATTATTGACCACTGTATAATCCGCAGCACATGCACAGTTGGTTCTGCAGTGTGTGTCAGTGGCCA GGGGGCGTGTCCCACTATCAAGCATTGTAACATCAGTGACTGTGAAAACGTCGGACTTTATATAACAGAT CATGCACAGGGAATATATGAAGATAATGAAATTTCTAATAATGCGTTAGCTGGGATTTGGGTTAAAAATC ATGGAAATCCAATTATTAGACGGAATCACATTCATCATGGACGTGATGTTGGTGTGTTCACATTTGATCA TGGCATGGGCTACTTTGAAAGTTGCAACATACACAGAAACAGGATAGCAGGCTTTGAGGTAAAAGCCTAT GCCAATCCCACAGTGGTTCGATGTGAGATTCACCACGGGCAGACTGGAGGAATATACGTCCACGAGAAAG GAAGAGGACAGTTCATAGAGAACAAGATCTATGCAAACAATTTCGCAGGCGTGTGGATTACCTCAAATAG TGACCCAACAATAAGGGGGAATTCTATATTTAATGGAAATCAAGGAGGAGTTTACATCTTTGGTGACGGA CGAGGCCTTATAGAAGGAAATGACATTTATGGTAATGCGTTAGCAGGAATTCAGATCAGGACAAACAGTT GTCCTATTGTTCGACATAACAAAATTCATGATGGACAGCATGGTGGGATTTATGTGCATGAAAAGGGGCA AGGAGTAATAGAAGAGAATGAAGTTTACAGCAACACTCTGGCTGGCGTCTGGGTGACAACTGGCAGCACA CCAGTTCTAAGAAGAAACCGGATACACAGTGGCAAGCAGGTTGGTGTTTATTTCTATGACAATGGACATG GTGTTCTAGAAGATAACGACATCTACAATCACATGTATTCAGGGGTTCAGATAAGAACTGGAAGCAACCC CAAAATTAGACGCAACAAAATTTGGGGAGGACAGAATGGTGGAATTCTAGTTTATAATTCTGGTCTAGGC TGTATAGAAGACAATGAAATATTTGACAACGCGATGGCTGGAGTCTGGATTAAAACAGATAGTAATCCTA CGCTAAGAAGAAATAAAATCCATGATGGAAGAGATGGTGGCATCTGTATATTTAATGGGGGTCGAGGTCT CCTTGAAGAAAATGATATTTTCAGGAACGCTCAAGCAGGTGTCCTCATCAGCACTAACAGTCATCCAGTA TTAAGGAAAAACAGAATATTTGATGGATTTGCTGCAGGTATTGAAATTACAAATCATGCAACTGCAACAC TCGAAGGCAATCAGATCTTTAACAACCGATTTGGAGGCTTATTTTTAGCATCTGGTGTTAATGTGACAAT GAAAGATAACAAAATAATGAACAATCAAGATGCCATAGAAAAGGCTGTTAGTAGAGGACAATGTCTATAT AAAATATCAAGTTATACCAGCTACCCCATGCATGACTTCTACAGATGTCATACTTGTAACACCACAGATC GAAATGCCATATGTGTGAACTGCATTAAGAAGTGCCATCAGGGACATGATGTAGAGTTCATTAGACATGA TAGGTTTTTCTGTGACTGTGGTGCTGGAACGCTGTCTAATCCTTGTACCTTAGCTGGCGAGCCTACACAC GACACAGACACACTCTATGACTCTGCCCCACCTATAGAATCTAATACGTTGCAGCACAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224533 representing NM_001081034
Red=Cloning site Green=Tags(s) MNSVRAANRRPRRVSRPRPVQQQQQQPPQQPPPQPPQQQPPPQPPQQPPQQQPPPPPQQQPPPPPPPPPP PPQDRNNAGERDDVPADMVAEESGPGAQNSPYQLRRKTLLPKRTACPTKSSMEGASTSTTENFGHRAKRA RVSGKSQDLSAAPAEQYLQEKLPDEVVLKIFSYLLEQDLCRAACVCKRFSELANDPILWKRLYMEVFEYT RPMMHPEPGKFYQINPEEYEHPNPWKESFQQLYKGAHVKPGFAEHFYSNPARYKGRENMLYYDTIEDALG GVQEAHFDGLIFVHSGIYTDEWIYIESPITMIGAAPGKVADKVIIENTRDSTFVFMEGSEDAYVGYMTIR FNPDDKSAQHHNAHHCLEITVNCSPIIDHCIIRSTCTVGSAVCVSGQGACPTIKHCNISDCENVGLYITD HAQGIYEDNEISNNALAGIWVKNHGNPIIRRNHIHHGRDVGVFTFDHGMGYFESCNIHRNRIAGFEVKAY ANPTVVRCEIHHGQTGGIYVHEKGRGQFIENKIYANNFAGVWITSNSDPTIRGNSIFNGNQGGVYIFGDG RGLIEGNDIYGNALAGIQIRTNSCPIVRHNKIHDGQHGGIYVHEKGQGVIEENEVYSNTLAGVWVTTGST PVLRRNRIHSGKQVGVYFYDNGHGVLEDNDIYNHMYSGVQIRTGSNPKIRRNKIWGGQNGGILVYNSGLG CIEDNEIFDNAMAGVWIKTDSNPTLRRNKIHDGRDGGICIFNGGRGLLEENDIFRNAQAGVLISTNSHPV LRKNRIFDGFAAGIEITNHATATLEGNQIFNNRFGGLFLASGVNVTMKDNKIMNNQDAIEKAVSRGQCLY KISSYTSYPMHDFYRCHTCNTTDRNAICVNCIKKCHQGHDVEFIRHDRFFCDCGAGTLSNPCTLAGEPTH DTDTLYDSAPPIESNTLQHN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081034 |
| ORF Size | 2790 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081034.3 |
| RefSeq Size | 4013 bp |
| RefSeq ORF | 2793 bp |
| Locus ID | 225055 |
| UniProt ID | Q7TPD1 |
| Gene Summary | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DTL/CDT2, BCL6 and PRDM1/BLIMP1. The SCF(FBXO11) complex mediates ubiquitination and degradation of BCL6, thereby playing a role in the germinal center B-cells terminal differentiation toward memory B-cells and plasma cells. The SCF(FBXO11) complex also mediates ubiquitination and degradation of DTL, an important step for the regulation of TGF-beta signaling, cell migration and the timing of the cell-cycle progression and exit. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of p53/TP53.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222715 | Fbxo11 (untagged) - Mouse F-box protein 11 (Fbxo11), (10ug) |
CNY 11,970.00 |
|
| MR224533 | Fbxo11 (Myc-DDK-tagged) - Mouse F-box protein 11 (Fbxo11) |
CNY 6,808.00 |
|
| MR224533L3 | Lenti ORF clone of Fbxo11 (Myc-DDK-tagged) - Mouse F-box protein 11 (Fbxo11) |
CNY 10,070.00 |
|
| MR224533L4 | Lenti ORF clone of Fbxo11 (mGFP-tagged) - Mouse F-box protein 11 (Fbxo11) |
CNY 10,070.00 |
