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Nin (NM_008697) Mouse Tagged ORF Clone
CAT＃: MG224478
- TrueORF®
Nin (tGFP-tagged) - Mouse ninein (Nin) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_008697" in other vectors (2)
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CNY 22,330.00
货期*
9周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3110068G20Rik; AI385615; AU024711; mKIAA1565 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224478 representing NM_008697
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAGGTGGAGGAGGACCAGCATGAAGCCCGACTCAAGGAGCTGTTTGACAGTTTTGACACCCTGG GAACTGGGTCTCTGGGTCAAGAGGAGCTCACTGACCTCTGCCACGTGCTGTGCTTGGAGGATGTGGGCCC GGTGCTGCAACAGACGCTACTCCAGGACAACCTCTTGGGCAGGGTACATTTTGACCAATTTAAAGAAGCA TTAATACTTATCTTGTCTAGAACTCTATCCAGTGAGGAGCACTTTGAGGAGTCAGACTGTTCCCCAGAAG CTCAGCCCAAATATGTTAGAGGTGGGAAGCGTTATGGACGAAGATCACTGCCCGAGTTTCAAGAGTCTGG GGAAGAGATCGAGGAGGTGACAGTGCTTGAGCCACTAGAGGAAGAAGCGAGGTCATCACCCATCCCAGCT GGGGACTGCGGCGAGCACTGGAAGACACAACGCAGTGAGGAGTATGAAGCAGAAGGCCAGCTGAGGTTTT GGAACCCAGATGACCTGAATGCTTCACATGGTGGGTCTTGCCCTCCTCCAGACTGGATAGAAGAGAAACT GCAAGAGGTTTGTGAAGATCTGGGGATCACTCGAGATGGTCACCTGAACCGGAAGAAGCTGGTTTCCATC TGCGAACAGTATGGATTACAGAATGTGGATGGAGCGATGCTGGAAGAGGTGTTTCTCAGCCTTGATCCCG ATGGAACAATGAGTGTGGAAGACTTTTTCTATGGTCTGTTTAAGACTGGAAAATCTCTCACACCATCAGC ATCTACTCCCTATAGACAACTGAAACGGCATCTCTCTATGCAGTCTTTCGATGAGAGTGGACGGCGGACC GCAACCTCGTCAGCAATGACGAGTACCATTGGCTTCCGGGTCTTCTCCTGCTTGGATGACGGGATGGGCC AGGCGTCTGTGGAGAGGATACTGGACACCTGGCAGGAAGAAGGCATTGAGAACAGTCAGGAGATTCTGAA GGCCCTGGATTTCAGCCTGGATGGAAACATCAACCTGACAGAGCTGACACTGGCTCTGGAGAATGAACTT TTGGTTACCAAGAATGGCATTCACCAGGCAGCACTAGCTAGCTTCAAGGCCGAGATCCGGCACTTGTTAG AACGAGTTGACCAAGTAGTCAGAGAAAAGGAGAAGCTACGGTCGGACCTGGACAAAGCAGAAAAGCTGAA GTCTCTCATGGCTTCCGAGGTGGACGATCACCACGCAGCCATTGAGCGGAGGAATGAATACAACCTCAGG AAACTGGATGAAGAATACAAGGAGCGCATAGCAGCCTTAAAGAACGAACTCCGCCAGGAGAGGGAACAGA TGTTGCAGCAAGTGGGCAAGCAGCGGGTGGAGCTGGAACAGGAAATTCAAAAGGCCAAGACGGAAGAGAA TTACATCCGGGACCGCCTTGCCCTCTCCCTGAAGGAAAACAATCGCCTGGAGACTGAGCTTCTGGAAAAC GCAGAGAAGTTGGCAGAGTACGAGAGCCTGACGCAGAAGCTCCAGCGCAGTTTGGAAAATGTGTTAGCAG AAAAGTTTGGTGACCTCGATCCCAGCAGTGCTGAGTTCTTTCTTCAAGAGGAGAGGCTGGCGCAGATGAG AAATGAATATGAGCAACAGTGCAGGCTATTACAAGACCAAGTGGATGAGCTTCAGTCTGAACTAGAAGAA TACCAGGCTCAAGGCAGGGTGCTCAGACTCCCCTTGAAGAACTCGCTGTCAGAAGAACTCGACGGTCACA GTGGTGGTATTGAGCCCGACCAAGGGCCTGGATCTGAAGAGTGCAACCCCCTGAATATGAGCATTGAGGC AGAGCTGGTCATCGAGCAGATGAAGGAGCAGCACCACCGGGACCTGTGTCACCTGAGACTGGAGCTGGAA GACAAAGTGCGCCACTATGAAAAGCAGCTGGATGATACCAGGGTCGCCTCTGAACAGGAGCAGGCGGCCA TGAAGCAGAAGTATGAGCAGGGAGTGCACACCTTGGAGAAGCGAGTCAGCGAGCTTCGGAGTGAGATTGC AGACCTGGAAGGGCAGGCAGCCGTGCTGAGGGAGGCCCACCACAAGGCCAGCTGCAGGCACGAGGAGGAA AAGAGACAACTGCAAATGGCGTTCGATGAGGAAAAGGCTCAGCTCCAGGAGGAGCTGCGGCAGGAGCATG AGAGGGAGCTCCAGGCCAGGCTGCAGCAGGCAGCGGAGAGCTTTCGCCAGGAGAGAGAGGGACTCGCCCA GGCTGCCTGGACTGAGGAGAAGGTGAGAGGCTTGGAGCAATCTTACCAGGAGCAGCTGCTGAGCCTGGAG GAGAAGCATGCTCTAGAGAAAGAAGAGCTGAGGGAAGAGCTCTCGGAACATCACCGAAGGGAACTCCAGG AGGGAAGGGAAGAAATGGAAACAGAGTGTAATAGAAGAGTCTCTCAGATAGAAGCCCAGTGCCAGGCTGA CTGTGAGAAAGTCACTGAGCACTGTGAACAAACTCTGCAAAGCCTCGAGGTTCGCCACCGCCAGGAGCTG AGGGACCTCTTGGACCAGCACCTGGAGGAAAGGTCTCAGTGGGAGTTTGAGAAGGATGAGCTTACACAAG AGTGCACAGATGCCCAGGAGCAGCTGAAGGAGGCGCTCCAGAGGGAGAGGGCGACCGCTGCGGCCATGAA GCAGGAACAGGAGATACTGGAGAGGACATACAAGGACCGTTTGAATATCCTGAGCACGGAAAGAGAGCAG CTGCTGCAAGACCTGAAAGACCTACAGAACGCATCTGAGAGCCAGCATGGCCTCCTGTCTGGCCAGATAC TTGAGCTGAAGAGAAGTCAGGAGAGAGAACTGAGGGACCAAGGGCAGGCGCTATGCCAGACGGGGGTCTC TGAGCAGCTGGCCAGCCAGCAGCTCGAAAGGCTCCGGGTTGAGCATGAGCAAGAGAGGCGAGAAATGACG GGCAAGCTTGCCGCCCTGGAGAGCGCCCACAGAGCAAGCCTTGAGAGAGCAGATCAAGAGAAGGCGGAGA TGAGCACAGAAATCTGCAGGCTTCAGAATACAGTCAAGGACATGCAGCAGGCAGCATCTCTCTTGATGCT TCAGGGTGGCTGCCAGGCAACGGCAGGGGAGGAGGCAGAAGGAGATGGAGCCATGTCCCTGCTTCAGCAA GGCGAGCAGCTATTGGAAGAAAACGGAGATGTCCTCATCAGCCTGCAGAGAGCTCACGAGCATGCAGTGA AGGAAAATGCAAAGATGGCTACTGAAATTTACCGATTGCAGCAGAGGCTGAAGAAGTTAGAGCCGGGGTC GGTGATCTCGTCTTGTTTGGAGGAGGGGACGTCTGAGATTTCTGGAAGTTCCAGGGAACAAGTAGAACCC ATAATGAAACAAGGTCCAGCCACCAAGCACTTCCTGAGCGATCTAGGAGACCACGAGGCCCGGGACCTGG CAAGTACCGGGACAAGCTCTGTTCAGAGGCAGGAGTGCAAAACCGAGGCGTCGGAAGCTTCCCTGGACTG CTTTTCTGAGCTTGAAAACAGTGAAGACACCAGGACCGAGTCCTGGGACCTCAAGAGTCAGATCAGCCAG CTTCGGGAGCAACTGACGGTCTTGCGAGCAGACTGCGATCGGGCTTCCGAGAGGAAACAGGACCTGCTTT TCGATATCTCTGTGCTGAAAAAGAAACTGAAGATGCTGGAGAGACTCCCGGAGGCATCTTCCAGGTATAA GGTATTATACGAGGATGCCGCCCGAGAAAACTCCTGCCTTCAGGAGGAGCTGAGACTTGTAGAGACGCGC TACGAGGAGTCGCTGGACAGCAACAAAGAGCTCACGGCGGAGGTCTACAGGTTGCAGGATGAGATGAAGA AAATGGAGGAGGTGATGGAGACGTTCCTCAGCCTGGAGAAGAGTTACGATGAGGTCAAAGTGGAAAATGA GGAACTCCGTGCTCTGGTGTTGAGACTTCAAGGGAAGATGGAGAAGGTGCTGGGAAGGGCCGCCCTGCAG GGTGACAGCTACGCCCTCTGGGAAGCCCCTTCAGAAAACCTGGAGGTCGCCTCGGATGAAAAGATGCTTG AACTCCGTCAGACTCCGAAAGAGTGTACGCCCAAGGTCGTGAGCATGCACCACATCATAGAAGAGTGTAC ACAAGAAACCCAGTGCTGTGAGCAGGGAAGCACGAAGCTCCTAGCTAGAATAAAAGCACACGAAATCGCC TGGTTCCACAGAGCAATTAAGACACATCCGGAAAAGCCTAGCGCGCAGAACCGAGTCATCCCGGAGGGAA GTGCTGCTCTCCTAGGCCTACAAGACAAGCATCTTCAGCAGGAGGCCACCATCGCAGAGTTAGAACTAGA GAAGCAAAAGCTACAGGAGCTGACTAGAAATCTGAGGGAGCGAGTCACTGCACTAGTTAGGCAAAAAGAC GCCCCTTCTCAAGGGCAGAAGGAGGAGGAGCTGAAGGCAATGATGCAGGACCTGCAGATCACGTGCGGGG AGATGCAGAGGAAGGTTGAACTCCTGAGATATGAATCTGAAAAGCTTCAAGAGGAAAATTCTATTTTGAG AAATGAAATTACTACTTTAAATGAAGAAGATAGCATCTCTAACCTGAAATTGGAGGAATTAAATGGCTCT CAGGAAGAATTGTGGCAGAAAATAGAAACCATAGAGCAGGAAAAAGCTTCAATTCAGACAATGGTTGAAA AATTAAAGAAACAGGTTTCAGATTTAAAAATCAAAAACCAACAGTTGGATTCAGAAAATATAGAACTCAG CCAAAAGAACTCCCAAAACAAGGAAGAACTGAAAACCCTTAATCAACGTCTGGCAGAAATGCTGTGCCAG AGGGAGGAGCCAGGAGCTTGCACCTCTGAGAAATGGGAACAAGAAAATGCAAGCCTCAAAGAAGAACTGG ATCACTACAAAGTGCAGACTTCCACTTTGGTGTCTTCTCTGGAGGCAGAACTTTCCGAAGTTAAACTGCA GACTCACGTCATGGAGCAGGAAAACCTCCTTCTCAAAGATGAACTGGAGAGACTGAAGCAACTGCACAGA TGTCCTGACCTCTCTGACTTCCAGCAAAAAATGTCTAGCATTCTAAGCTACAATGAAAAACTGCTGAAGG AAAAAGAAGTTCTGAGTGAAGAGTTAAAGAGCTGTGCAGATAAGCTGGCAGAGTCAAGCCTCTTAGAGCA CAGAATTGCTACAATGAAGCAGGAGCAGACCGCCTGGGAAGAGCAGAGTGAGAGCCTGAAGTCACAGCTG GCAGTGTCGCAGGCAAAGGTTCAGAACTTAGAAGATGTCCTGCAGAATGTCAACCTTCAGATGGCTGAGA TTGAATCAGATCTCCAAGTGACTCGACAGGAAAAGGAGGCGCTAAAGCAGGAAGTGATGTCATTACACAG ACAGCTTCAGAATGCCATTGATAAGGACTGGGTCTCGGAAACAGCTCCCCATCTCTCAGGGCTCCGGGGC CAGCAGAGAAGGCTGTCCTGGGACAAACTGGACCATCTGATGAATGAGGAACCACAGCTGTTTTGTCAAG AAAGCAAGAGACTCCAGACTGTGGTACAGAATACCCAAGCAGATCTCACCCACTCCCGGGAGAAGGTCCG TCAACTGGAATCCAACCTCCTTCCCACCAAACACCAAAAACAACTCAACCAACCATGTACTGTGAAGTCC ACAGAACAAGAAAAACTGACCTTAAAGAGAGAGTGTGAACAGTCTCAGAAAGAACAATCTCCTACCAGCA GGAAGGTGGGTCAGATGGGTTCCCTTGAGCGAGGATTAGAAACAATCCATTTGGAAAACGAAGGTCTGAA GAAGAAACAGGTGAGACTGGACGAGAAGCTAATGGAGATGCAGCCCCTGAGGTCCACTGTGACGCGTAGC CCATCCTCTCACTGGGATCTGCAGTTGCTCCAGCAGCAAGCCTGTCCGATGGTGCCCAGGGAGCAGTTTC TGCAGCTTCAACAGCAGCTGCTGCAGGCAGAAAAGAGAAGCCAGCACCTGCAGGAGGAGCTCGAAAACAG AACCTCCGAAACCAACACACCACAGGCTTTGCTACTGGAGCAGCGAGCTGTGCATGCAGATTCCTGCAGA AGGATTGGGCACCTC ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224478 representing NM_008697
Red=Cloning site Green=Tags(s) MDEVEEDQHEARLKELFDSFDTLGTGSLGQEELTDLCHVLCLEDVGPVLQQTLLQDNLLGRVHFDQFKEA LILILSRTLSSEEHFEESDCSPEAQPKYVRGGKRYGRRSLPEFQESGEEIEEVTVLEPLEEEARSSPIPA GDCGEHWKTQRSEEYEAEGQLRFWNPDDLNASHGGSCPPPDWIEEKLQEVCEDLGITRDGHLNRKKLVSI CEQYGLQNVDGAMLEEVFLSLDPDGTMSVEDFFYGLFKTGKSLTPSASTPYRQLKRHLSMQSFDESGRRT ATSSAMTSTIGFRVFSCLDDGMGQASVERILDTWQEEGIENSQEILKALDFSLDGNINLTELTLALENEL LVTKNGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLR KLDEEYKERIAALKNELRQEREQMLQQVGKQRVELEQEIQKAKTEENYIRDRLALSLKENNRLETELLEN AEKLAEYESLTQKLQRSLENVLAEKFGDLDPSSAEFFLQEERLAQMRNEYEQQCRLLQDQVDELQSELEE YQAQGRVLRLPLKNSLSEELDGHSGGIEPDQGPGSEECNPLNMSIEAELVIEQMKEQHHRDLCHLRLELE DKVRHYEKQLDDTRVASEQEQAAMKQKYEQGVHTLEKRVSELRSEIADLEGQAAVLREAHHKASCRHEEE KRQLQMAFDEEKAQLQEELRQEHERELQARLQQAAESFRQEREGLAQAAWTEEKVRGLEQSYQEQLLSLE EKHALEKEELREELSEHHRRELQEGREEMETECNRRVSQIEAQCQADCEKVTEHCEQTLQSLEVRHRQEL RDLLDQHLEERSQWEFEKDELTQECTDAQEQLKEALQRERATAAAMKQEQEILERTYKDRLNILSTEREQ LLQDLKDLQNASESQHGLLSGQILELKRSQERELRDQGQALCQTGVSEQLASQQLERLRVEHEQERREMT GKLAALESAHRASLERADQEKAEMSTEICRLQNTVKDMQQAASLLMLQGGCQATAGEEAEGDGAMSLLQQ GEQLLEENGDVLISLQRAHEHAVKENAKMATEIYRLQQRLKKLEPGSVISSCLEEGTSEISGSSREQVEP IMKQGPATKHFLSDLGDHEARDLASTGTSSVQRQECKTEASEASLDCFSELENSEDTRTESWDLKSQISQ LREQLTVLRADCDRASERKQDLLFDISVLKKKLKMLERLPEASSRYKVLYEDAARENSCLQEELRLVETR YEESLDSNKELTAEVYRLQDEMKKMEEVMETFLSLEKSYDEVKVENEELRALVLRLQGKMEKVLGRAALQ GDSYALWEAPSENLEVASDEKMLELRQTPKECTPKVVSMHHIIEECTQETQCCEQGSTKLLARIKAHEIA WFHRAIKTHPEKPSAQNRVIPEGSAALLGLQDKHLQQEATIAELELEKQKLQELTRNLRERVTALVRQKD APSQGQKEEELKAMMQDLQITCGEMQRKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLEELNGS QEELWQKIETIEQEKASIQTMVEKLKKQVSDLKIKNQQLDSENIELSQKNSQNKEELKTLNQRLAEMLCQ REEPGACTSEKWEQENASLKEELDHYKVQTSTLVSSLEAELSEVKLQTHVMEQENLLLKDELERLKQLHR CPDLSDFQQKMSSILSYNEKLLKEKEVLSEELKSCADKLAESSLLEHRIATMKQEQTAWEEQSESLKSQL AVSQAKVQNLEDVLQNVNLQMAEIESDLQVTRQEKEALKQEVMSLHRQLQNAIDKDWVSETAPHLSGLRG QQRRLSWDKLDHLMNEEPQLFCQESKRLQTVVQNTQADLTHSREKVRQLESNLLPTKHQKQLNQPCTVKS TEQEKLTLKRECEQSQKEQSPTSRKVGQMGSLERGLETIHLENEGLKKKQVRLDEKLMEMQPLRSTVTRS PSSHWDLQLLQQQACPMVPREQFLQLQQQLLQAEKRSQHLQEELENRTSETNTPQALLLEQRAVHADSCR RIGHL TRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008697 |
| ORF Size | 6105 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008697.2, NP_032723.2 |
| RefSeq Size | 6708 bp |
| RefSeq ORF | 6108 bp |
| Locus ID | 18080 |
| UniProt ID | Q61043 |
| Gene Summary | Centrosomal protein required for the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15784680, PubMed:10934040). May also act as a centrosome maturation factor (By similarity). May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15784680). Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (By similarity). Required for centriole organization and microtubule anchoring at the mother centriole (By similarity).[UniProtKB/Swiss-Prot Function] |
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