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Mprip (NM_012027) Mouse Tagged ORF Clone
CAT＃: MG224193
- TrueORF®
Mprip (tGFP-tagged) - Mouse myosin phosphatase Rho interacting protein (Mprip) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_012027" in other vectors (4)
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CNY 11,310.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9530046C02; AA536749; AI647711; C76423; mKIAA0864; p116Rip; Rhoip3; RIP3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224193 representing NM_012027
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGCCAAGGAAAACCCGTGCAGGAAATTTCAGGCCAACATCTTCAACAAGAGCAAGTGTCAGA ACTGCTTCAAGCCCCGCGAGTCGCATCTGCTCAACGACGAGGACCTGACGCAGGCAAAACCCATTTATGG TGGCTGGCTGCTCCTGGCTCCAGATGGCACTGACTTTGACAACCCAGTGCACCGGTCACGGAAATGGCAG CGACGATTCTTCATCCTTTATGAGCATGGCCTCTTGCGATATGCCCTGGATGAGATGCCCACCACCCTCC CTCAGGGCACCATCAACATGAACCAGTGCACAGATGTGGTGGATGGGGAGGCCCGCACAGGGCAGAAGTT CTCCCTCTGCATCCTGACACCTGACAAGGAACATTTTATCAGGGCAGAAACCAAGGAGATCATCAGTGGG TGGTTAGAGATGCTCATGGTGTATCCTCGAACCAACAAGCAGAACCAGAAGAAGAAACGGAAAGTAGAGC CACCTACCCCACAGGAGCCTGGGCCTGCTAAGATGGCTGTCACTAGCAGTAGTGGCGGCAGCAGTGGTAG CAGCAGCAGCATTCCCAGTGCTGAGAAAGTTCCCACCACCAAATCCACACTCTGGCAGGAAGAAATGAGA GCCAAAGACCAACCTGATGGGACCAGCCTGAGTCCAGCTCAAAGTCCCAGCCAAAGCCAGCCTCCTGCTG CTTGCACCCCACGGGAACCAGGACTAGAAAGCAAAGAAGATGAAAGCACCATAAGTGGGGACCGTGTGGA TGGTGGTCGGAAAGTACGGGTAGAGAGCGGCTACTTCTCCCTGGAGAAGGCCAAGCAGGACCTGAGGGCT GAGGAGCAGCTGCCCCCACTACTCTCCCCACCCAGCCCCAGCACCCCACACAGCAGGAGGTCCCAGGTAA TTGAGAAATTTGAGGCCTTGGACATTGAAAAAGCAGAGCACATGGAAACCAACATGCTGATTCTGACCAC TCCATCAAGTGACACTCGTCAGGGCCGCAGTGAGAGACGGGCCATCCCTAGAAAGCGGGACTTTGCCAGC GAAGCCCCCACAGCTCCTCTCTCAGATGCTTGTCCCCTGTCTCCACACCGAAGAGCCAAGTCATTGGACA GGAGGTCCACAGAATCCTCCATGACGCCTGACCTGCTGAATTTCAAGAAAGGCTGGCTAACCAAGCAGTA TGAGGATGGCCAGTGGAAGAAACACTGGTTTGTCCTGGCAGATCAGAGCCTGAGATACTACAGGGATTCC GTGGCTGAGGAGGCAGCTGACCTAGATGGAGAAATTAACTTGTCCACTTGCTATGATGTCACTGAGTATC CAGTCCAGAGAAACTATGGCTTTCAGATACATACCAAGGAGGGTGAGTTCACCCTCTCAGCCATGACATC TGGCATACGACGGAACTGGATCCAGACCATTATGAAACATGTGCTCCCAGCATCTGCCCCAGATGTGACC AGCTCATTGCCTGAGGGAAAGAATAAAAGCACATCTTTTGAGACCTGCTCAAGGTCAACTGAGAAGCAAG AAGCTGAGCCAGGAGAGCCAGATCCTGAACAAAAGAAGAGCCGTGCTCGAGAGCGGAGGCGGGAGGGTCG CTCCAAGACCTTTGACTGGGCTGAATTCCGCCCTATACAACAGGCCCTGGCTCAAGAAAGGGCCAGCGCT GTTGGGTCTTCTGATTCTGGTGACCCTGGGTGTCTTGAAGCCGAGCCAGGTGAGCTGGAGCGTGAACGAG CTCGACGTCGAGAAGAACGCCGCAAGCGCTTTGGGATGCTGGACACCATTGATGGACCAGGAATGGAGGA CACAGCCCTACGTATGGACATCGACCGGAGCCCAGGGCTGCTGGGAACCCCTGACCTCAAGACACAGAAT GTCCACGTGGAAATTGAGCAGCGTTGGCATCAAGTGGAGACCACCCCTCTCAGGGAGGAGAAACAGGTGC CCATTGCCCCTCTGCACCTATCCTTGGAGGACAGAAGTGAGCGGCTCTCCACACATGAGCTCACCTCTCT GCTAGAGAAGGAGCTGGAGCAGAGCCAGAAAGAAGCCTCGGATCTTCTAGAGCAGAACCGGCTTCTACAG GACCAGTTAAGGGTGGCCCTGGGCAGAGAACAGAGTGCCCGGGAGGGCTATGTGCTGCAGGCTACATGTG AGCGAGGCTTTGCCGCCATGGAGGAGACACATCAGAAGAAGATTGAAGACCTACAGAGGCAACACCAGCG GGAGCTAGAGAAGCTGCGGGAGGAAAAAGACCGCCTCCTGGCTGAGGAGACAGCTGCCACCATATCAGCC ATTGAAGCCATGAAGAATGCTCACCGAGAGGAGATGGAACGAGAGTTAGAGAAGAGCCAGCGGTCTCAGA TCAGCAGCATCAATTCAGACATAGAAGCCCTGAGGCGACAGTACCTGGAGGAGCTGCAGTCAGTGCAGCG GGAGCTGGAAGTCCTTTCAGAGCAGTACTCCCAAAAGTGCCTGGAGAATGCACACCTAGCCCAGGCGCTG GAGGCCGAGCGTCAGGCCCTGCGGCAATGCCAGCGTGAGAACCAGGAGCTCAACGCCCACAACCAGGAGC TGAATAACCGCCTGGCTGCGGAGATCACACGGTTGCGGACACTACTGACTGGAGACGGTGGTGGGGAGTC CACTGGGTTGCCTCTCACACAGGGCAAGGATGCCTATGAGTTAGAGGTCTTGTTGCGGGTCAAAGAGTCA GAAATCCAGTACCTGAAACAGGAGATCAGCTCCCTCAAGGATGAACTCCAGACAGCCCTGCGGGATAAGA AGTATGCTAGTGACAAGTACAAAGACATCTACACTGAGCTCAGCATTGCCAAGGCCAAGGCTGACTGCGA CATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCACTGGGCGAGAAATCTCCTGAAGGCACT ACTGTGTCAGGATATGACATAATGAAATCTAAAAGCAATCCTGACTTCCTGAAGAAAGACAGATCCTGTG TTACCCGGCAACTCAGAAACATCAGGTCCAAGTCCGTAATTGAGCAGGTCTCATGGGATAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224193 representing NM_012027
Red=Cloning site Green=Tags(s) MSAAKENPCRKFQANIFNKSKCQNCFKPRESHLLNDEDLTQAKPIYGGWLLLAPDGTDFDNPVHRSRKWQ RRFFILYEHGLLRYALDEMPTTLPQGTINMNQCTDVVDGEARTGQKFSLCILTPDKEHFIRAETKEIISG WLEMLMVYPRTNKQNQKKKRKVEPPTPQEPGPAKMAVTSSSGGSSGSSSSIPSAEKVPTTKSTLWQEEMR AKDQPDGTSLSPAQSPSQSQPPAACTPREPGLESKEDESTISGDRVDGGRKVRVESGYFSLEKAKQDLRA EEQLPPLLSPPSPSTPHSRRSQVIEKFEALDIEKAEHMETNMLILTTPSSDTRQGRSERRAIPRKRDFAS EAPTAPLSDACPLSPHRRAKSLDRRSTESSMTPDLLNFKKGWLTKQYEDGQWKKHWFVLADQSLRYYRDS VAEEAADLDGEINLSTCYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVLPASAPDVT SSLPEGKNKSTSFETCSRSTEKQEAEPGEPDPEQKKSRARERRREGRSKTFDWAEFRPIQQALAQERASA VGSSDSGDPGCLEAEPGELERERARRREERRKRFGMLDTIDGPGMEDTALRMDIDRSPGLLGTPDLKTQN VHVEIEQRWHQVETTPLREEKQVPIAPLHLSLEDRSERLSTHELTSLLEKELEQSQKEASDLLEQNRLLQ DQLRVALGREQSAREGYVLQATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATISA IEAMKNAHREEMERELEKSQRSQISSINSDIEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQAL EAERQALRQCQRENQELNAHNQELNNRLAAEITRLRTLLTGDGGGESTGLPLTQGKDAYELEVLLRVKES EIQYLKQEISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPEGT TVSGYDIMKSKSNPDFLKKDRSCVTRQLRNIRSKSVIEQVSWDN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012027 |
| ORF Size | 3072 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012027.2, NP_036157.2 |
| RefSeq Size | 8784 bp |
| RefSeq ORF | 3075 bp |
| Locus ID | 26936 |
| UniProt ID | P97434 |
| Gene Summary | Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223236 | Mprip (untagged) - Mouse myosin phosphatase Rho interacting protein (Mprip), transcript variant 2, (10ug) |
CNY 13,210.00 |
|
| MR224193 | Mprip (Myc-DDK-tagged) - Mouse myosin phosphatase Rho interacting protein (Mprip), transcript variant 2 |
CNY 10,260.00 |
|
| MR224193L3 | Lenti ORF clone of Mprip (Myc-DDK-tagged) - Mouse myosin phosphatase Rho interacting protein (Mprip), transcript variant 2 |
CNY 12,160.00 |
|
| MR224193L4 | Lenti ORF clone of Mprip (mGFP-tagged) - Mouse myosin phosphatase Rho interacting protein (Mprip), transcript variant 2 |
CNY 12,160.00 |
