Herc6 (NM_025992) Mouse Tagged ORF Clone
CAT#: MG224065
- TrueORF®
Herc6 (tGFP-tagged) - Mouse hect domain and RLD 6 (Herc6), (10ug)
ORF Plasmid: DDK
"NM_025992" in other vectors (4)
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CNY 11,020.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1700121D12Rik; 2510038N07Rik; 4930427L17Rik; AI451296; CEB1; Herc5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224065 representing NM_025992
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTTCAGCTGGGCTGCAGGAAGCCGGAAGCCTCGGCGCCTGAAGGCGGGAACCAGCGGCATTGAAC TCTTGCAGGCAGCCAGCGGGGAACACCACTCGCTCCTACTGTTCAGCAACCACAGGGTGTATTCCTGCGG GGACAACAGCTGGGGACAGCTGGGCCAGAGGAGAGACCAGAGTACCGAAAGGCCAGAACCAATTCAGGCA CTGAATGATCTGCATGTTGACCTGGTGAGCTGTGGGAAGGAACACTCTGTGGCTGTCTGCCACAAAGGAA AGGTCTTCGCTTGGGGAGCCGGTTCTGAAGGGCAGCTGGGGATTGGAGAATTTAAGGAAATAAGCTTTAT GCCTACGAAAATAAAAGCTCTGGCTGGCATAAAAATAATCCAAGTTTCCTGTGGACACTACCACTCTTTG GCCTTATCAGAAGATGGCCACGTGTTTTCATGGGGAAGGAACAGTGAAGGACAACTGGGCCTGGGGAAGA ATTCCCGTTCTCAAGCTATTCCACAGAAGGTGAAGTCTCTGGAGGGGATCCCACTGGCTCAGGTTGCTGC AGGTGGCACACACAGTTTTGCTCTGTCTCTCACGGGGACATCATTTGGCTGGGGGAGCAACAGATCTGGG CAGTTGGCTCTCAGCGGGAACAAAGTTAAAGAACAAATCTACAAGCCGCATTCAATTGGTGCCCTGAAGA ATCTGAGTGTGATTTATATTAGCTGTGGTTATGAGCATACTGCAGTGCTTACTGAGGAGGGACAAGTGTT TACATTTGGAGGCAATAGCTCTGGACAACTACAGCCCAGCCCCAGGAGTGGACAAAGAGGCCCACAACTC ATAGAAGGAATTGGTGGCCGTGTTTCACAGATAGAGTGCGCAAGTTATCACACCATTGCATATGTCTACA CCACTGGTCAGGTGGTATCTTTAGGCCGTGGACCAAGTCACACAAGCAACCCAACTCATCAGGAGGCCCC CGCAGAGAACTCTGATATTACCTGCCTGCTAGCTGCAGAGGACCTTGTGGATATTGAAGTCAAAGACATT TTTGCTGGAGCACACGCCAACTTTGTGACAACTCGTCGGGTATCACATACTAGGTCTACAGGTGTTTCCA TGAAAATCCTGCCAGAAATAATCCGAATCAACCAGTCTCTGGTAAAGAAATGGAGAGCAGCAAATAAAAG AAAAGATCGTGAAGGCGCCAAAAGAGAAATCAGCTTGATATTTTCATCTTCTGCTTGTTTGACAGCGAGC TTTCTGAAGAAAAGAGATGCTGGAGAGAATAATCTCATTGATGTGGACTTAAAACTGGCAAGAGATGTCT TCAAGAAGTTAACAACAGAGAAATGGATTTCTTCTTTGATAACCACGTGTCTTGAGGAGTATCTGCTCAG AGATCTTCCATACAGTTCTCCACACCAAGAAGCTCTACTTGTTTTTCTTTTGCTCCCAGAATGTTCCATT ATGCAGGATCCTAAGAACTGGAAGACCCTGGCATTTGAATTTGCAAAAGCTATTCATAAAATGGGTCCAC AGTCATTAGCATTCCTGAGAACGTGTTGGGCATCTTTGGAAGTTTCTTCACTGAACATACTGGTTCAGAT GCTAAAGAAAGCCATCATCTCTCAAATACAGTATGGAGTTGCAACGGAGCAGTATATTACTAATATTAAA GTTCTTTTAGAAGTGATAAAAGAAGTGCATAAGGCTAACTGTCAACTTCCAGAAAGTGCCTTCATCATAA ATGAGCTTTCTGGCATCTTTAACTTTGATGCAGAGGCAGGAAGAATGTTCATACGGCATAACGATCTGGA CTGTACAGAATCCTCAGATATGGTTGTTTTCAGTGATTTTCTATTTGTCTTTGATTTGCCATCCAAAATT AAACTAATGAAATGTGACTCATTTGTAAAACTGATGTCTGAAGTCATGGCATTTCCAGAAAAGATGAGCT CCCCACCATACCTTATACTGAAAGTCAGACGAAGTCACCTGGTGGAAGACACATTGCGTCAATTAAGACA AGTGGAAGACTTTGACCTTAGGAAGCAACTATCGGTTGGATTTATTAATGAAATTCGTCCTGAGGCTGGG GGAGTGAGTTCAGAGTTCTTCCACTGTATATTTGAAGAGATGACAGACCCCAAATATGAAATGTTCATAT ATCCTGAAAAGGGTTCCAGCATGTGGTTTCCAGTCAATCCCAAGTTTGAGAAGAGCAGCTATTTCCTATT TGGGATCTTATGTGGACTCTCCCTACATAACTTAAAGGTTATCAACCTTCCTTTCCCACTGGCTTTATAT AAAAAACTTTTGAACCAAAAGCCATCTTTGGAAGACTTAAAAGAACTTAGTCTTCCCTTGGGGAGGAATT TGCAAGAAGTTCTAAATTGTGAGGCTGGTGATATTGAAGAACTTCACATGTATTTTTCTATATACTGGGA TCAAAAAGATGTTGATTTAATTCCAGATGGGATCTCTGTGCCTGTGAATGAAACCAACAAGAGAGACTAT GTGTCTAAGTATGTTGATTACATTTTTAACATCTCTATAAAGACGATTTATGAAGAATTTCATAGAGGAT TTTACAAAGTGTGTAACTGGGATATAATTAGGCAATTCCAGCCCGAAGAACTAATGACAGCAATAATTGG AAATGCTACTTGTGACTGGAAACAATTTGAAAATAATTCAAAGTATAAAGATGGATATGACAAATCACAC CCAACTATTCTGCTGTTTTGGAAGGCTTTCCATGACTTAACTTTGGATGAAAAGAAAAAATTCCTCTTGT TTCTTACAGGATGTGATAGGCTGCATGTGAAAGGCCTACAGAATGAAGGAATAGTATTCCGGTGTTCTGA AACCTTCAGTGAAGAAGATAACCCAAGATCATTGACATGTCACAGGATGCTAGACCTACCTAAGTATTCC TCAATGCGAAGGATGAAGGAAGCACTTCAGGTTGCAATCAACAATAGCACGGGATTTGTCTCACAGGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224065 representing NM_025992
Red=Cloning site Green=Tags(s) MYFSWAAGSRKPRRLKAGTSGIELLQAASGEHHSLLLFSNHRVYSCGDNSWGQLGQRRDQSTERPEPIQA LNDLHVDLVSCGKEHSVAVCHKGKVFAWGAGSEGQLGIGEFKEISFMPTKIKALAGIKIIQVSCGHYHSL ALSEDGHVFSWGRNSEGQLGLGKNSRSQAIPQKVKSLEGIPLAQVAAGGTHSFALSLTGTSFGWGSNRSG QLALSGNKVKEQIYKPHSIGALKNLSVIYISCGYEHTAVLTEEGQVFTFGGNSSGQLQPSPRSGQRGPQL IEGIGGRVSQIECASYHTIAYVYTTGQVVSLGRGPSHTSNPTHQEAPAENSDITCLLAAEDLVDIEVKDI FAGAHANFVTTRRVSHTRSTGVSMKILPEIIRINQSLVKKWRAANKRKDREGAKREISLIFSSSACLTAS FLKKRDAGENNLIDVDLKLARDVFKKLTTEKWISSLITTCLEEYLLRDLPYSSPHQEALLVFLLLPECSI MQDPKNWKTLAFEFAKAIHKMGPQSLAFLRTCWASLEVSSLNILVQMLKKAIISQIQYGVATEQYITNIK VLLEVIKEVHKANCQLPESAFIINELSGIFNFDAEAGRMFIRHNDLDCTESSDMVVFSDFLFVFDLPSKI KLMKCDSFVKLMSEVMAFPEKMSSPPYLILKVRRSHLVEDTLRQLRQVEDFDLRKQLSVGFINEIRPEAG GVSSEFFHCIFEEMTDPKYEMFIYPEKGSSMWFPVNPKFEKSSYFLFGILCGLSLHNLKVINLPFPLALY KKLLNQKPSLEDLKELSLPLGRNLQEVLNCEAGDIEELHMYFSIYWDQKDVDLIPDGISVPVNETNKRDY VSKYVDYIFNISIKTIYEEFHRGFYKVCNWDIIRQFQPEELMTAIIGNATCDWKQFENNSKYKDGYDKSH PTILLFWKAFHDLTLDEKKKFLLFLTGCDRLHVKGLQNEGIVFRCSETFSEEDNPRSLTCHRMLDLPKYS SMRRMKEALQVAINNSTGFVSQE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025992 |
ORF Size | 3009 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025992.2, NP_080268.1 |
RefSeq Size | 5279 bp |
RefSeq ORF | 3012 bp |
Locus ID | 67138 |
UniProt ID | F2Z461 |
Gene Summary | Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223151 | Herc6 (untagged) - Mouse hect domain and RLD 6 (Herc6), (10ug) |
CNY 12,920.00 |
|
MR224065 | Herc6 (Myc-DDK-tagged) - Mouse hect domain and RLD 6 (Herc6) |
CNY 10,472.00 |
|
MR224065L3 | Lenti ORF clone of Herc6 (Myc-DDK-tagged) - Mouse hect domain and RLD 6 (Herc6) |
CNY 11,880.00 |
|
MR224065L4 | Lenti ORF clone of Herc6 (mGFP-tagged) - Mouse hect domain and RLD 6 (Herc6) |
CNY 11,880.00 |