Gpr132 (NM_019925) Mouse Tagged ORF Clone
CAT#: MG223944
- TrueORF®
Gpr132 (tGFP-tagged) - Mouse G protein-coupled receptor 132 (Gpr132), (10ug)
ORF Plasmid: DDK
"NM_019925" in other vectors (4)
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CNY 7,088.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | G2a |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223944 representing NM_019925
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGATCAGAACCTACCAATGCAGCAGGAAACACCACACTGGGGGTTACCTCCGTTCTTCAGAGCACCT CAGTACCTTCTTCTGAGACCTGCCACGTCTCCTACGAGGAGAGCAGAGTGGTCCTGGTGGTGGTGTACAG TGCCGTGTGCCTGCTGGGCCTACCAGCCAACTGCCTGACTGCCTGGCTGACGCTGCTGCAAGTCCTGCAG AGGAACGTGCTAGCCGTCTACCTGTTCTGCCTGTCCCTCTGTGAGCTGCTCTACATCAGCACGGTGCCAT TGTGGATCATCTACATCCAGAATCAGCACAAATGGAACCTGGGTCCGCAGGCCTGCAAGGTGACTGCTTA CATCTTCTTCTGCAACATCTACATCAGCATCCTCTTGCTCTGCTGCATTTCCTGCGACCGCTACATGGCC GTGGTCTATGCACTGGAGAGCCGAGGCCACCGCCACCAGAGGACTGCTGTCACCATTTCTGCGTGTGTGA TTCTTCTTGTTGGACTTGTTAACTATCCAGTGTTTGACATGAAGGTGGAGAAGAGTTTCTGCTTTGAGCC CCTGAGGATGAACAGCAAGATAGCCGGCTACCACTACCTGCGTTTCACCTTTGGCTTTGCCATCCCTCTC GGCATCCTGGCGTTCACCAATCACCAGATCTTCCGGAGCATCAAACTCAGTGACAGCCTGAGCGCTGCGC AGAAGAACAAGGTGAAGCGCTCCGCCATCGCGGTCGTCACCATCTTCCTGGTCTGCTTTGCTCCCTACCA CGTGGTACTCCTCGTCAAAGCTGCCAGCTTTTCCTTCTACCAAGGAGACATGGATGCCGTGTGTGCCTTT GAAAGCAGACTGTACACAGTCTCTATGGTGTTTCTGTGCCTGTCTACAGTCAACAGTGTGGCTGACCCCA TCATCTACGTGCTGGGTACAGACCACTCTCGGCAAGAAGTGTCCAGAATCCACACAGGGTGGAAAAAGTG GTCCACAAAGACATATGTTACATGCTCAAAGGACTCTGAGGAGACACACTTGCCCACAGAGCTTTCAAAC ACATACACCTTCCCCAATCCCGCGCACCCTCCAGGATCACAGCCAGCGAAGCTAGGTTTACTGTGCTCGC CAGAGAGACTGCCTGAGGAGCTCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223944 representing NM_019925
Red=Cloning site Green=Tags(s) MRSEPTNAAGNTTLGVTSVLQSTSVPSSETCHVSYEESRVVLVVVYSAVCLLGLPANCLTAWLTLLQVLQ RNVLAVYLFCLSLCELLYISTVPLWIIYIQNQHKWNLGPQACKVTAYIFFCNIYISILLLCCISCDRYMA VVYALESRGHRHQRTAVTISACVILLVGLVNYPVFDMKVEKSFCFEPLRMNSKIAGYHYLRFTFGFAIPL GILAFTNHQIFRSIKLSDSLSAAQKNKVKRSAIAVVTIFLVCFAPYHVVLLVKAASFSFYQGDMDAVCAF ESRLYTVSMVFLCLSTVNSVADPIIYVLGTDHSRQEVSRIHTGWKKWSTKTYVTCSKDSEETHLPTELSN TYTFPNPAHPPGSQPAKLGLLCSPERLPEELC TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_019925 |
ORF Size | 1146 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019925.4, NP_064309.1 |
RefSeq Size | 2439 bp |
RefSeq ORF | 1149 bp |
Locus ID | 56696 |
UniProt ID | Q9Z282 |
Gene Summary | May be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May function as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. May mediate ths accumulation of intracellular inositol phosphates at acidic pH through proton-sensing activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210087 | Gpr132 (untagged) - Mouse G protein-coupled receptor 132 (Gpr132), (10ug) |
CNY 3,656.00 |
|
MR223944 | Gpr132 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 132 (Gpr132) |
CNY 5,488.00 |
|
MR223944L3 | Lenti ORF clone of Gpr132 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 132 (Gpr132) |
CNY 5,230.00 |
|
MR223944L4 | Lenti ORF clone of Gpr132 (mGFP-tagged) - Mouse G protein-coupled receptor 132 (Gpr132) |
CNY 5,230.00 |