Mmel1 (NM_013783) Mouse Tagged ORF Clone
CAT#: MG223375
- TrueORF®
Mmel1 (tGFP-tagged) - Mouse membrane metallo-endopeptidase-like 1 (Mmel1), (10ug)
ORF Plasmid: DDK
"NM_013783" in other vectors (4)
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CNY 6,370.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Mell1; NEP2; NEPII; Nl1; SEP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223375 representing NM_013783
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGAGAGAGCAGGCTGGTGTCGGAAGAAGTCCCCAGGCTTCGTGGAGTATGGGCTGATGGTGCTGC TGCTGCTGCTGTTGCTGGGAGCCATAGTGACTCTGGGTGTCTTCTACAGCATAGGGAAGCAGCTGCCCCT CTTAACTAGCCTGCTACACTTCTCCTGGGATGAGAGGACGGTTGTAAAACGAGCCCTCAGGGATTCATCA CTGAAAAGTGACATCTGCACCACCCCAAGCTGTGTGATAGCAGCTGCCAGAATCCTCGAAAACATGGACC AATCGAGGAACCCCTGTGAAAACTTCTACCAGTACGCTTGCGGAGGCTGGCTGAGGCACCACGTGATCCC AGAGACCAACTCCAGATACAGCGTCTTTGACATCCTTCGGGACGAGCTGGAGGTTATCCTCAAAGGGGTG CTGGAGGATTCCACTTCCCAGCATCGCCCGGCCGTGGAGAAGGCCAAGACACTATATCGCTCCTGCATGA ACCAAAGTGTGATCGAGAAGAGAGACTCTGAGCCCCTGCTGAGCGTCTTAAAAATGGTAGGAGGTTGGCC TGTGGCCATGGATAAGTGGAACGAGACCATGGGCCTCAAGTGGGAACTGGAGCGACAGTTGGCTGTGTTG AACTCGCAGTTCAACAGGCGGGTCCTCATCGACCTCTTCATCTGGAATGACGACCAGAACTCCAGCCGGC ATGTCATCTACATAGACCAGCCCACCTTGGGCATGCCATCCCGGGAGTACTATTTCCAGGAGGACAACAA CCACAAGGTACGGAAAGCCTACCTGGAGTTCATGACGTCAGTGGCCACTATGCTTAGGAAAGACCAGAAC CTGTCCAAGGAGAGCGCCATGGTGCGGGAGGAGATGGCGGAGGTGCTGGAACTGGAGACGCATCTGGCCA ACGCCACAGTCCCCCAGGAGAAAAGGCATGATGTCACTGCCCTGTACCACCGAATGGACCTGATGGAGCT ACAGGAAAGGTTTGGTCTGAAGGGGTTTAACTGGACTCTCTTCATACAAAACGTGTTGTCTTCTGTGGAA GTCGAGCTGTTCCCAGATGAGGAGGTGGTGGTCTACGGCATCCCCTACCTGGAGAATCTGGAGGATATCA TTGATAGCTACTCAGCACGGACCATGCAGAACTACCTGGTATGGCGCCTGGTGCTAGATCGAATTGGCAG CCTGAGCCAGAGATTCAAAGAGGCGCGTGTGGACTACCGCAAGGCGCTGTACGGCACGACCGTGGAGGAG GTACGCTGGCGAGAGTGTGTCAGCTATGTCAACAGTAACATGGAGAGCGCCGTGGGCTCCCTCTACATCA AGCGGGCCTTCTCCAAGGACAGCAAGAGCACGGTCAGAGAGCTGATTGAGAAGATAAGGTCCGTGTTTGT GGATAACCTGGATGAGCTGAACTGGATGGACGAGGAATCCAAGAAGAAGGCCCAGGAAAAGGCCATGAAT ATACGGGAACAGATTGGCTACCCTGACTACATTTTGGAAGATAACAATAAACACCTGGATGAGGAATACT CCAGTTTGACTTTCTATGAGGACCTGTATTTTGAGAACGGACTTCAGAACCTCAAGAACAATGCCCAGAG GAGCCTCAAGAAGCTTCGGGAAAAGGTGGACCAGAATCTCTGGATCATCGGGGCTGCAGTGGTCAATGCA TTCTACTCCCCAAACAGAAACCAGATCGTCTTTCCAGCAGGGATTCTCCAGCCGCCCTTCTTCAGCAAGG ACCAACCACAGTCCTTGAATTTTGGGGGCATCGGGATGGTGATTGGGCACGAGATCACACACGGCTTTGA TGATAATGGTCGTAACTTTGACAAGAACGGCAACATGCTGGACTGGTGGAGTAACTTCTCGGCCCGGCAC TTCCAACAGCAGTCGCAATGCATGATCTATCAGTACGGCAACTTCTCTTGGGAACTAGCAGACAACCAGA ATGTGAACGGATTCAGTACCCTCGGGGAGAACATTGCCGACAACGGAGGTGTGCGACAGGCATACAAGGC TTACCTACGGTGGCTGGCTGATGGCGGCAAAGATCAGCGACTGCCGGGACTGAACCTGACCTATGCCCAG CTTTTCTTCATCAACTATGCCCAGGTGTGGTGTGGGTCCTATAGGCCGGAGTTCGCCGTCCAGTCCATCA AGACGGACGTCCACAGTCCTCTTAAGTACAGGGTGCTGGGCTCACTACAGAACCTGCCAGGCTTCTCTGA GGCATTCCACTGCCCACGAGGCAGCCCCATGCACCCCATGAAGCGATGTCGCATCTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223375 representing NM_013783
Red=Cloning site Green=Tags(s) MVERAGWCRKKSPGFVEYGLMVLLLLLLLGAIVTLGVFYSIGKQLPLLTSLLHFSWDERTVVKRALRDSS LKSDICTTPSCVIAAARILENMDQSRNPCENFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGV LEDSTSQHRPAVEKAKTLYRSCMNQSVIEKRDSEPLLSVLKMVGGWPVAMDKWNETMGLKWELERQLAVL NSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFQEDNNHKVRKAYLEFMTSVATMLRKDQN LSKESAMVREEMAEVLELETHLANATVPQEKRHDVTALYHRMDLMELQERFGLKGFNWTLFIQNVLSSVE VELFPDEEVVVYGIPYLENLEDIIDSYSARTMQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTVEE VRWRECVSYVNSNMESAVGSLYIKRAFSKDSKSTVRELIEKIRSVFVDNLDELNWMDEESKKKAQEKAMN IREQIGYPDYILEDNNKHLDEEYSSLTFYEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGAAVVNA FYSPNRNQIVFPAGILQPPFFSKDQPQSLNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARH FQQQSQCMIYQYGNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLRWLADGGKDQRLPGLNLTYAQ LFFINYAQVWCGSYRPEFAVQSIKTDVHSPLKYRVLGSLQNLPGFSEAFHCPRGSPMHPMKRCRIW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013783 |
ORF Size | 2298 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013783.2, NP_038811.2 |
RefSeq Size | 2948 bp |
RefSeq ORF | 2301 bp |
Locus ID | 27390 |
UniProt ID | Q9JLI3 |
Gene Summary | Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221441 | Mmel1 (untagged) - Mouse membrane metallo-endopeptidase-like 1 (Mmel1), (10ug) |
CNY 9,880.00 |
|
MR223375 | Mmel1 (Myc-DDK-tagged) - Mouse membrane metallo-endopeptidase-like 1 (Mmel1) |
CNY 5,608.00 |
|
MR223375L3 | Lenti ORF clone of Mmel1 (Myc-DDK-tagged) - Mouse membrane metallo-endopeptidase-like 1 (Mmel1) |
CNY 7,700.00 |
|
MR223375L4 | Lenti ORF clone of Mmel1 (mGFP-tagged) - Mouse membrane metallo-endopeptidase-like 1 (Mmel1) |
CNY 7,700.00 |