Wnt11 (NM_009519) Mouse Tagged ORF Clone

CAT#: MG223281

  • TrueORF®

Wnt11 (tGFP-tagged) - Mouse wingless-related MMTV integration site 11 (Wnt11), (10ug)

ORF Plasmid: DDK tGFP



  "NM_009519" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-Wnt11 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG223281 representing NM_009519
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGCGCGGCCGCAGGTCTGCGAGGCTCTGCTCTTTGCCTTGGCGCTCCACACCGGCGTGTGCTATG
GCATCAAGTGGCTGGCACTGTCCAAGACTCCGGCAGCCTTGGCACTGAATCAGACGCAACACTGTAAACA
GCTGGAGGGCCTGGTGTCTGCGCAGGTGCAGCTCTGCCGCAGCAACCTGGAGCTCATGCGCACCATCGTG
CACGCCGCCCGGGGGGCCATGAAGGCCTGCCGTAGGGCCTTCGCTGACATGCGCTGGAACTGCTCCTCCA
TCGAGCTCGCCCCCAACTACCTGCTTGACCTGGAGAGAGGTACACGGGAGTCAGCCTTCGTGTATGCCCT
GTCGGCCGCCACCATCAGTCACACCATCGCCCGGGCCTGCACCTCTGGCGACCTGCCCGGCTGCTCCTGC
GGCCCCGTCCCAGGTGAGCCACCCGGGCCCGGGAACCGCTGGGGAGGATGTGCGGACAACCTCAGCTACG
GGCTCCTCATGGGGGCCAAGTTTTCCGATGCTCCTATGAAGGTGAAAAAAACAGGATCCCAAGCCAATAA
ACTGATGCGTCTACACAACAGTGAAGTGGGGAGACAGGCTCTACGTGCCTCCCTGGAAACGAAGTGTAAA
TGCCATGGGGTGTCTGGCTCCTGCTCCATCCGCACCTGTTGGAAGGGGCTGCAAGAGCTCCAGGACGTGG
CTGCTGACCTCAAGACCCGCTACCTGTCAGCCACGAAGGTGGTACACCGGCCTATGGGCACCCGCAAACA
CTTGGTGCCCAAGGACCTGGATATCCGGCCTGTGAAGGACTCAGAACTTGTGTATCTACAGAGCTCCCCT
GACTTCTGCATGAAGAATGAGAAGGTGGGATCCCATGGGACCCAAGACAGGCAGTGCAACAAGACTTCCA
ACGGCAGTGACAGCTGCGACCTCATGTGCTGTGGGCGCGGCTACAACCCCTACACGGACAGAGTGGTGGA
GCGATGTCACTGCAAGTACCACTGGTGCTGCTACGTCACCTGCCGCAGGTGTGAGCGCACGGTGGAGCGC
TACGTCTGCAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG223281 representing NM_009519
Red=Cloning site Green=Tags(s)

MRARPQVCEALLFALALHTGVCYGIKWLALSKTPAALALNQTQHCKQLEGLVSAQVQLCRSNLELMRTIV
HAARGAMKACRRAFADMRWNCSSIELAPNYLLDLERGTRESAFVYALSAATISHTIARACTSGDLPGCSC
GPVPGEPPGPGNRWGGCADNLSYGLLMGAKFSDAPMKVKKTGSQANKLMRLHNSEVGRQALRASLETKCK
CHGVSGSCSIRTCWKGLQELQDVAADLKTRYLSATKVVHRPMGTRKHLVPKDLDIRPVKDSELVYLQSSP
DFCMKNEKVGSHGTQDRQCNKTSNGSDSCDLMCCGRGYNPYTDRVVERCHCKYHWCCYVTCRRCERTVER
YVCK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009519
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009519.2, NP_033545.1
RefSeq Size 2788 bp
RefSeq ORF 1065 bp
Locus ID 22411
UniProt ID P48615
Gene Summary Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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