Tmie (NM_146260) Mouse Tagged ORF Clone
CAT#: MG223273
- TrueORF®
Tmie (tGFP-tagged) - Mouse transmembrane inner ear (Tmie), (10ug)
ORF Plasmid: DDK
"NM_146260" in other vectors (4)
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CNY 2,850.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 5131400L21Rik; Mm.87012; sr |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223273 representing NM_146260
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGAGGCAGCATGGCTCGGGGCGGCTCTGGGCGCTGGGCGGCGCCGCGCTGGGGGCTTGCCTGG CGGGTGTCGCCACGCAGCTGGTAGAGCCCAGCACCGCACCGCCAAAGCCCAAGCCGCCCCCACTGACCAA GGAGACTGTGGTGTTCTGGGACATGCGCCTGTGGCACGTGGTGGGCATCTTTTCGCTCTTCGTGTTGTCC ATCATCATCACGCTATGCTGTGTCTTCAACTGCCGGGTGCCACGGACGCGGAAGGAGATCGAGGCTCGGT ATCTACAGCGAAAGGCGGCCAAGATGTACACAGACAAGCTGGAGACTGTGCCTCCCCTTAATGAACTCAC AGAAATCCCTGGAGAGGACAAGAAGAAAAAGAAGAAGGACAGTGTGGACACAGTGGCTATCAAGGTAGAG GAGGACGAGAAGAATGAGGCGAAGAAGAAAGGAGAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223273 representing NM_146260
Red=Cloning site Green=Tags(s) MAGRQHGSGRLWALGGAALGACLAGVATQLVEPSTAPPKPKPPPLTKETVVFWDMRLWHVVGIFSLFVLS IIITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEIPGEDKKKKKKDSVDTVAIKVE EDEKNEAKKKGEK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_146260 |
ORF Size | 459 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_146260.2, NP_666372.1 |
RefSeq Size | 2437 bp |
RefSeq ORF | 462 bp |
Locus ID | 20776 |
UniProt ID | Q8K467 |
Gene Summary | Unknown. The protein may play some role in a cellular membrane location. May reside within an internal membrane compartment and function in pathways such as those involved in protein and/or vesicle trafficking. Alternatively, the mature protein may be localized in the plasma membrane and serve as a site of interaction for other molecules through its highly charged C-terminal domain.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209463 | Tmie (untagged) - Mouse transmembrane inner ear (Tmie), (10ug) |
CNY 1,200.00 |
|
MR223273 | Tmie (Myc-DDK-tagged) - Mouse transmembrane inner ear (Tmie) |
CNY 1,200.00 |
|
MR223273L3 | Lenti ORF clone of Tmie (Myc-DDK-tagged) - Mouse transmembrane inner ear (Tmie) |
CNY 4,750.00 |
|
MR223273L4 | Lenti ORF clone of Tmie (mGFP-tagged) - Mouse transmembrane inner ear (Tmie) |
CNY 4,750.00 |