Crhr1 (NM_007762) Mouse Tagged ORF Clone
CAT#: MG222960
- TrueORF®
Crhr1 (tGFP-tagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1), (10ug)
ORF Plasmid: DDK
"NM_007762" in other vectors (4)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CRF; CRF-R; CRF1R; CRFR1; Crhr |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222960 representing NM_007762
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACAGCGCCCGCAGCTCCGGCTCGTGAAGGCCCTTCTCCTTCTGGGGCTGAACCCCGTCTCCACCT CCCTCCAGGATCAGCAGTGTGAGAGCCTGTCCCTGGCCAGCAATGTCTCTGGCCTGCAGTGCAATGCCTC CGTGGACCTCATTGGCACCTGCTGGCCCAGGAGCCCTGCAGGGCAGTTGGTGGTTCGGCCCTGCCCTGCC TTTTTCTACGGTGTCCGCTACAACACCACAAACAATGGCTACCGGGAATGCCTGGCCAACGGCAGCTGGG CAGCCCGTGTGAATTATTCTGAGTGCCAGGAGATTCTCAACGAAGAGAAGAAGAGCAAAGTGCACTACCA CATTGCCGTCATCATCAACTACCTGGGCCACTGCATCTCCCTGGTGGCCCTCCTGGTGGCCTTTGTCCTC TTCCTGCGGCTCAGGAGCATCCGGTGCCTGAGGAACATCATCCACTGGAACCTCATCTCGGCTTTCATCC TGCGCAACGCCACGTGGTTTGTGGTCCAGCTCACCGTGAGCCCCGAGGTCCACCAGAGCAACGTGGCCTG GTGCAGGCTGGTGACAGCCGCCTACAACTACTTCCACGTAACCAACTTCTTCTGGATGTTCGGTGAGGGC TGCTACCTGCACACAGCCATCGTACTCACGTACTCCACCGACCGTCTGCGCAAGTGGATGTTCGTCTGCA TCGGCTGGGGTGTGCCTTTCCCCATCATTGTGGCTTGGGCCATTGGGAAACTTTACTACGACAATGAAAA GTGCTGGTTTGGCAAACGTCCTGGAGTATATACTGACTACATCTACCAGGGCCCCATGATCCTGGTCCTG CTGATCAACTTTATCTTTCTCTTCAACATTGTCCGCATCCTCATGACCAAACTCCGAGCATCCACCACAT CTGAGACTATTCAGTACAGGAAGGCTGTGAAGGCCACTCTGGTGCTCCTGCCCCTCCTGGGCATCACCTA CATGTTGTTCTTCGTCAACCCTGGGGAGGACGAGGTCTCCAGGGTTGTCTTCATCTACTTCAACTCTTTC CTGGAGTCCTTTCAGGGCTTCTTCGTGTCTGTGTTCTATTGTTTTCTGAACAGTGAGGTCCGCTCTGCCA TCCGGAAGAGGTGGCGGCGGTGGCAGGACAAGCACTCCATCAGAGCCCGAGTGGCCCGCGCCATGTCCAT CCCCACCTCCCCCACCAGAGTCAGCTTCCACAGCATCAAGCAGTCCACAGCAGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222960 representing NM_007762
Red=Cloning site Green=Tags(s) MGQRPQLRLVKALLLLGLNPVSTSLQDQQCESLSLASNVSGLQCNASVDLIGTCWPRSPAGQLVVRPCPA FFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKKSKVHYHIAVIINYLGHCISLVALLVAFVL FLRLRSIRCLRNIIHWNLISAFILRNATWFVVQLTVSPEVHQSNVAWCRLVTAAYNYFHVTNFFWMFGEG CYLHTAIVLTYSTDRLRKWMFVCIGWGVPFPIIVAWAIGKLYYDNEKCWFGKRPGVYTDYIYQGPMILVL LINFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSF LESFQGFFVSVFYCFLNSEVRSAIRKRWRRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007762 |
ORF Size | 1245 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007762.4, NP_031788.1 |
RefSeq Size | 2428 bp |
RefSeq ORF | 1248 bp |
Locus ID | 12921 |
UniProt ID | P35347 |
Gene Summary | The protein encoded by this gene is a G-protein coupled receptor that binds corticotropin-releasing factor (CRH) and urocortin (UCN) with high affinity. The encoded protein upregulates adenylate cyclase activity and intracellular cAMP levels. This protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response, and obesity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208333 | Crhr1 (untagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1), (10ug) |
CNY 3,656.00 |
|
MR222960 | Crhr1 (Myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1) |
CNY 3,656.00 |
|
MR222960L3 | Lenti ORF clone of Crhr1 (Myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1) |
CNY 4,750.00 |
|
MR222960L4 | Lenti ORF clone of Crhr1 (mGFP-tagged) - Mouse corticotropin releasing hormone receptor 1 (Crhr1) |
CNY 6,056.00 |