Dmc1 (NM_010059) Mouse Tagged ORF Clone
CAT#: MG222831
- TrueORF®
Dmc1 (tGFP-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog meiosis-specific homologous recombination (yeast) (Dmc1), (10ug)
ORF Plasmid: DDK
"NM_010059" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Dmc1h; Lim15; Mei1; Mei11; sg; sgdp |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222831 representing NM_010059
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAGGATCAAGTTGTGCAGGAAGAATCGGGATTCCAAGATGATGAGGAATCTCTGTTTCAAGACA TTGACCTGTTGCAGAAGCATGGGATTAACATGGCTGATATTAAGAAACTGAAGTCTGTAGGAATCTGTAC CATTAAAGGGATACAAATGACAACAAGAAGAGCCCTCTGCAATGTCAAAGGCCTCTCAGAAGCCAAAGTG GAAAAGATTAAAGAGGCAGCCAACAAGCTTATTGAACCAGGATTCCTAACTGCATTTCAGTACAGTGAAA GGAGGAAGATGGTATTCCATATCACTACTGGGAGCCAGGAATTTGATAAATTACTAGGAGGTGGCATTGA AAGTATGGCAATTACAGAAGCTTTTGGAGAATTTCGTACTGGAAAAACTCAGCTGTCTCATACCCTCTGT GTGACAGCTCAACTTCCAGGAACAGGCGGCTACTCAGGTGGAAAGATTATCTTCATCGATACAGAAAATA CTTTCCGTCCAGATCGCCTTCGGGACATTGCTGACCGCTTCAACGTAGACCATGAAGCAGTACTGGACAA CGTGCTCTATGCACGTGCCTATACTAGTGAGCATCAGATGGAGCTACTTGATTATGTCGCAGCCAAGTTT CATGAGGAAGCTGGCATTTTCAAACTATTGATCATTGACTCAATAATGGCACTTTTTCGAGTGGATTTCA GTGGCCGTGGGGAATTGGCTGAACGCCAGCAAAAATTGGCCCAGATGTTGTCACGACTCCAAAAAATCTC AGAAGAATATAATGTTGCTGTTTTTGTGACCAATCAAATGACTGCAGATCCAGGAGCAACTATGACCTTT CAGGCTGATCCCAAAAAACCCATTGGTGGACACATTCTGGCTCACGCTTCCACAACAAGAATAAGCTTGC GCAAGGGAAGAGGAGAACTGAGGATCGCCAAGATTTATGACAGCCCCGAGATGCCTGAAAATGAAGCCAC CTTCGCAATAACCGCTGGAGGGATCGGGGATGCCAAGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222831 representing NM_010059
Red=Cloning site Green=Tags(s) MKEDQVVQEESGFQDDEESLFQDIDLLQKHGINMADIKKLKSVGICTIKGIQMTTRRALCNVKGLSEAKV EKIKEAANKLIEPGFLTAFQYSERRKMVFHITTGSQEFDKLLGGGIESMAITEAFGEFRTGKTQLSHTLC VTAQLPGTGGYSGGKIIFIDTENTFRPDRLRDIADRFNVDHEAVLDNVLYARAYTSEHQMELLDYVAAKF HEEAGIFKLLIIDSIMALFRVDFSGRGELAERQQKLAQMLSRLQKISEEYNVAVFVTNQMTADPGATMTF QADPKKPIGGHILAHASTTRISLRKGRGELRIAKIYDSPEMPENEATFAITAGGIGDAKE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010059 |
ORF Size | 1020 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010059.3, NP_034189.1 |
RefSeq Size | 2192 bp |
RefSeq ORF | 1023 bp |
Locus ID | 13404 |
UniProt ID | Q61880 |
Gene Summary | This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. In mouse, deficiency of this gene causes infertility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208400 | Dmc1 (untagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1), (10ug) |
CNY 3,990.00 |
|
MR222831 | Dmc1 (Myc-DDK-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1) |
CNY 3,656.00 |
|
MR222831L3 | Lenti ORF clone of Dmc1 (Myc-DDK-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1) |
CNY 5,230.00 |
|
MR222831L4 | Lenti ORF clone of Dmc1 (mGFP-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1) |
CNY 5,230.00 |