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Unc13b (NM_021468) Mouse Tagged ORF Clone
CAT＃: MG222713
- TrueORF®
Unc13b (tGFP-tagged) - Mouse unc-13 homolog B (C. elegans) (Unc13b) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_021468" in other vectors (4)
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CNY 15,390.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Munc13-1; Munc13-2; Unc13a; Unc13h1; Unc13h2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222713 representing NM_021468
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCTGCTCTGTGTGCGTGTTAAAAGAGCCAAATTCCAGGGTTCACCAGATAAATTTAACACATATG TGACCCTGAAAGTCCAGAACGTGAAGAGCACAACTGTAGCAGTTCGTGGTGATCAGCCTTCCTGGGAGCA GGATTTCATGTTTGAGATCAGTCGTCTGGACCTGGGCCTAAGTGTGGAGGTGTGGAACAAGGGACTGATA TGGGACACAATGGTGGGCACCGTGTGGATTGCACTGAAGACAATCCGTCAGTCGGATGAGGAAGGGCCCG GGGAGTGGTCTACACTGGAGGCAGAGACGTTAATGAAAGATGATGAGATCTGTGGAACTAAAAACCCAAC TCCTCATAAAATCTTGCTTGATACAAGATTTGAGCTGCCTTTTGACATCCCAGAGGAGGAAGCCAGATAT TGGACCTACAAATTGGAGCAAATCAATGCCTTGGCAGATGATAATGAGTATTCTAGTCAAGAAGAAAGCC AGAGGAAGCCATTACCCACTGCTGCCGCCCAGTGTTGTCACTGGACCTATTTGGGCTGGGGAGAGCATCA GACCTTTGAAGACCCTGATAGTGCCGTTGATGACCGAGACAGTGACTACCGCAGTGAGACCAGCAATAGC GCCCCACCTCCTTACCACACGACTACCCAGCCCAACGCTTCTGTGCACCAGTTCCCTGTTCCTGTGCGGC TGCCACAGCAGCTCTTTCTTCAAGGCAGTTCCCATGACTCTTGCAATGACTCTATGCAGAGTTACGACCT TGATTATCCAGAGCGTCGGGCCCTCAGCCCTACCAGCAGTAGTAGGTATGGCTCCTCCTGTAATGTGAGT CAAGGAAGCTCTCTGCTGAGTGAGCTGGACCAGTATCATGAGCAAGATGATGATGGTCGGGAGAGGGACT CCATTCATTCTAGTCATAGCTATGGCAGCCTCTCCAAAGATGGCCAGGCTGGTTTGGGAGAACAAGAGAA AGCCTTGGAAGTGACATGTGAATCAGAAAAAGAGAAAACAGGTGAATCCAAGGAGATGAGAGATGATGCT ACAATCTACCCTCCTTCAGATCTGGTGCTGCATAAGGACCATGTCCTAGGACCCCAGGAGAGTCTTCCTG AGGAGACCGCATCATCGCCATTTACCCAGGCCAGAGCACACTGGTTCCGAGCGGTTACCAAGGTCCGACT CCAACTGCAGGAGATTTCAGATGATGGTGACCCCTCCTTGCCTCAGTGGCTCCCAGAAGGGCCAGCTGGA GGGCTCTATGGCATCGACAGCATGCCAGATCTACGAAGGAAGAAGCCACTGCCACTTGTCAGTGATCTGT CACTGGTCCAGTCACGGAAAGCTGGGATTACATCTGCTATGGCTACACGTACCTCTCTCAAGGATGAAGA ACTGAAATCTCATGTGTATAAGAAAACTCTGCAGGCCTTAATCTACCCCATCTCGTGTACCACGCCCCAC AACTTTGAGGTCTGGTCTGCCACTACACCTACCTATTGCTATGAGTGTGAAGGCTTGCTCTGGGGCCTTG CCCGGCAAGGCATGCGCTGCAGCGAGTGTGGAGTCAAGTGCCATGAGAAGTGCCAGGACCTGCTCAATGC TGACTGCCTACAGCGGGCTGCTGAAAAGAGCTCTAAGCATGGAGCTGAGGACCGAACTCAGAACATTATC ATGGCCATGAAGGACCGTATGAAGATCCGAGAACGGAATAAACCAGAGATCTTTGAGGTTATCCGGGATG TGTTCACAGTGAGCAAAGTTGCGCATGTGCAGCAGATGAAAACAGTAAAGCAGAGTGTCCTGGATGGCAC CTCCAAGTGGTCAGCCAAAATTACCATCACGGTGGTTTGTGCCCAGGGCCTACAAGCCAAAGACAAGACA GGATCCAGTGACCCTTATGTGACTGTGCAAGTTGGGAAAACCAAGAAGCGCACCAAGACCATTTTTGGAA ATTTGAATCCTGTTTGGGAAGAGAAGTTCCATTTTGAGTGCCACAACTCTTCTGATCGAATTAAGGTACG CGTGTGGGATGAGGATGATGACATCAAGTCCAGAGTAAAGCAGCGGCTAAAGCGAGAGTCAGATGATTTC CTTGGCCAAACTATCATTGAAGTTCGAACGCTGAGTGGAGAGATGGATGTCTGGTACAATCTGGAGAAGA GAACAGACAAGTCAGCTGTCTCAGGGGCTATCCGACTTCAAATCAGTGTGGAGATCAAGGGGGAGGAGAA GGTAGCCCCATACCATGTACAATATACATGTCTCCATGAGAACCTTTTCCATTACCTCACAGACATTCAG GGCAGTGGAGGAGTCTGGATCCCAGAGGCTCGGGGGGACGATGCATGGAAGGTGTACTTTGATGAGACAG CCCAAGAAATTGTGGATGAATTCGCTATGCGCTATGGCATCGAGTCCATTTATCAGGCCATGACGCACTT TGCATGTTTGTCATCCAAGTACATGTGTCCTGGTGTACCAGCCGTGATGAGCACCTTACTGGCCAACATC AATGCCTATTATGCTCACACAACTGCCTCCACTAACGTCTCTGCATCTGATCGTTTTGCAGCCTCCAACT TTGGTAAAGAAAGATTTGTAAAACTGCTGGACCAGCTACACAACTCACTGAGGATAGACCTCTCTACATA CAGGAATAACTTTCCTGCTGGGAGCCCTGAGCGGCTTCAGGATTTAAAGTCTACAGTGGATTTGCTGACC AGCATTACTTTCTTCAGGATGAAGGTGCAAGAACTTCAGAGCCCTCCAAGAGCCAGCCAGGTGGTAAAGG ACTGTGTGAAAGCCTGCTTGAACTCTACATATGAATACATCTTCAACAACTGCCATGACCTCTACAGTCA CCAGTATCAGCTGGAACAACCTCTAGAGGAACCAGGGCCCAGCATTCGGAACTTGGATTTCTGGCCCAAA CTTATCACACTCATTGTTTCGATCATAGAGGAGGATAAGAATTCCTACACACCTGTTCTGAGCCAGTTTC CTCAGGAGTTGAACGTAGGAAAAGTCAGTGCAGAAGTGATGTGGCATCTGTTTGCTCAAGACATGAAATA TGCACTGGAGGAACACGAGAAAGACCGGCTGTGTAAGAGTGCTGACTACATGAACCTGCACTTCAAGGTG AAGTGGCTCCACAATGAATATGTGCGCGATCTGCCTGCCCTCCAGGGGCAAGTGCCTGAGTACCCAGCGT GGTTTGAGCAGTTTGTGCTACAGTGGCTAGATGAGAATGAAGATGTGTCACTGGAATTCCTTCGTGGGGC CTTGGAACGAGATAAGAAGGATGGGTTCCAGCAGACATCAGAGCATGCCTTGTTTTCCTGTTCTGTGGTG GACGTCTTTACACAACTCAACCAGAGCTTTGAGATCATCCGGAAGTTGGAATGTCCAGACCCCAACATCC TTGCCCACTACATGAGAAGGTTTGCTAAGACCATTGGGAAAGTGCTGATGCAGTATGCAGACATCTTATC AAAGAACTTCCCAGCTTACTGCACAAAGGAGAGACTGCCCTGTATCCTGATGAACAACATGCAGCAACTG AGGGTCCAGCTGGAGAAAATGTTTGAGGCCATGGGAGGCAAAGAGCTGGACTCTGAAGCTGCAGACAGTC TGAAGGAGCTGCAGGTGAAACTGAATACAGTTCTGGATGAGCTTAGCATGGTGTTTGGAAACAGCTTCCA GGTGCGGATTGATGAATGTGTTCGACAAATGGCTGACATCCTGGGCCAAGTTCGGGGCACAGGGAATGCA TCCCCTAACGCCAGGGCCTCGGTGGCTCAGGATGCAGATAGTGTGCTGCGACCTCCCATGGACTTCCTGG ATGGCAACCTCACGCTGTTTGCCACTGTGTGTGAGAAGACAGTTCTGAAGCGGGTCCTGAAGGAGCTCTG GCGGGTGGTGATGAACACCATGGAGCGGGTTATTGTCCTGCCCCCACTCACTGACCAGACGGGCACCCAG CTGATCTTAACTGCTGCCAAGGAGCTGAGCCAGCTTTCCAAACTCAAGGACCACATGGTACGGGAGGAAA CACGGAATCTCACTCCAAAGCAGTGTGCTGTCCTCGACTTGGCCTTGGACACCATCAAGCAATACTTCCA TGCAGGAGGCAATGGTCTGAAGAAAACCTTCCTGGAAAAGAGCCCAGACCTGCAGTCTCTGCGTTATGCC CTCTCTCTGTATACACAGACTACAGACACTCTCATCAAGACATTTGTGCGCTCACAGACTGCCCAGGGGG CTGGTGTGGATGACCCCGTGGGAGAAGTCTCTATTCAGGTGGACTTATTTACACATCCTGGCACTGGGGA GCATAAGGTCACAGTGAAAGTGGTAGCAGCCAATGACCTCAAGTGGCAGACAGCGGGTATGTTCCGGCCT TTTGTGGAAGTGACCATGGTTGGCCCACACCAAAGTGATAAGAAGAGGAAGTTCACAACCAAGTCTAAAA GTAACAACTGGACTCCCAAGTACAACGAGACGTTTCACTTCCTCCTGGGAAATGAAGAGGGTCCAGAGGC CTATGAATTGCAGATCTGTGTGAAGGATTACTGCTTTGCCCGGGAGGATCGTGTGATAGGACTGGCAGTG ATGCCCCTGAGGGATGTGGCAGCTAAGGGCAGCTGTGCCTGCTGGTGCCCACTGGGCCGGAAGATCCATA TGGATGAGACAGGCATGACCATTCTCCGGATTCTGTCTCAGAGGAGCAATGACGAGGTAGCCCGAGAGTT CGTGAAGCTCAAATCAGAGTCTCGATCCACAGAGGAAGGGAGC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222713 representing NM_021468
Red=Cloning site Green=Tags(s) MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLI WDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTKNPTPHKILLDTRFELPFDIPEEEARY WTYKLEQINALADDNEYSSQEESQRKPLPTAAAQCCHWTYLGWGEHQTFEDPDSAVDDRDSDYRSETSNS APPPYHTTTQPNASVHQFPVPVRLPQQLFLQGSSHDSCNDSMQSYDLDYPERRALSPTSSSRYGSSCNVS QGSSLLSELDQYHEQDDDGRERDSIHSSHSYGSLSKDGQAGLGEQEKALEVTCESEKEKTGESKEMRDDA TIYPPSDLVLHKDHVLGPQESLPEETASSPFTQARAHWFRAVTKVRLQLQEISDDGDPSLPQWLPEGPAG GLYGIDSMPDLRRKKPLPLVSDLSLVQSRKAGITSAMATRTSLKDEELKSHVYKKTLQALIYPISCTTPH NFEVWSATTPTYCYECEGLLWGLARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNII MAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKT GSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDF LGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQ GSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANI NAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSTYRNNFPAGSPERLQDLKSTVDLLT SITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYSHQYQLEQPLEEPGPSIRNLDFWPK LITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKV KWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVV DVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQL RVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNA SPNARASVAQDADSVLRPPMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQ LILTAAKELSQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYA LSLYTQTTDTLIKTFVRSQTAQGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRP FVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAV MPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESRSTEEGS SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021468 |
| ORF Size | 4806 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021468.2 |
| RefSeq Size | 6302 bp |
| RefSeq ORF | 4809 bp |
| Locus ID | 22249 |
| UniProt ID | Q9Z1N9 |
| Gene Summary | Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP) (By similarity). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224734 | Unc13b (untagged) - Mouse unc-13 homolog B (C. elegans) (Unc13b), transcript variant 1, (10ug) |
CNY 20,520.00 |
|
| MR222713 | Unc13b (Myc-DDK-tagged) - Mouse unc-13 homolog B (C. elegans) (Unc13b), transcript variant 1 |
CNY 10,960.00 |
|
| MR222713L3 | Lenti ORF clone of Unc13b (Myc-DDK-tagged) - Mouse unc-13 homolog B (C. elegans) (Unc13b), transcript variant 1 |
CNY 15,870.00 |
|
| MR222713L4 | Lenti ORF clone of Unc13b (mGFP-tagged) - Mouse unc-13 homolog B (C. elegans) (Unc13b), transcript variant 1 |
CNY 15,870.00 |
