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Spag16 (NM_029160) Mouse Tagged ORF Clone
CAT＃: MG222592
- TrueORF®
Spag16 (tGFP-tagged) - Mouse sperm associated antigen 16 (Spag16) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_029160" in other vectors (4)
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CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4921511D23Rik; 4930524F24Rik; 4930585K05Rik; AV261009; Pf20; Wdr29 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222592 representing NM_029160
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTCCGTCTGGGGTCCCGCCGCTGCGGGTGCTGGAGGAGCTGGGCATTGGCTTGTCACCCACCG GCGAGGTGACAGAGGCGGTGACCTCCGAGGGCGCCTACTACCTGGAGCAGGTCACCATAACGGAAACATC GGAAGATGAATGCGAATATGAAGAGATACCAGATGACAATTTCAGCATTCCTGAAGGAGAAGAAGATCTG GAAAAAGCAATTCACATCATTGGAGAGCAAGCCAGAGACATCCATATTTTGGAACAACAAACCATTCTTC CTGCAAGGAATGTAATGCAGGAAGCCATAGAAGATTTTCTCTGCAATTTCCTGATCAAAATGGGGATGAC CAGAACTCTGGACTGCTTCCAGGCCGAGTGGTATGAGTTAATACAGAAGAAAGGAAGTGACTTTAAAGGG CTCGGTAATGTTCCCGATGTCTACAGCCAGGTGATGCTTTTAGAGACTGAGAACAAAAACTTGAAGAAGG AATTGAAGCACTTCAAACAGGCAGCTGAAAAGGCCAAAGAAGATCTACTGAGAACTCAGAAGGAACGGGA CTTCCATCGCATGCATCATAAGCGCATAGTGCAAGAAAAGAACAAGCTCATTGCTGACCTCAAAGGGCTG AAATTGCATTATGCATCATACGAACCAACTATAAGGGTGTTACATGAGAAGCATCATGCTTTATTGAAGG AGAAAATGCTGACCTCTTTGGAAAGAGATCGAGCTGTTGGGAAGATTTCTGGGCTTCAAGCAACACTGAA GAACATAGACATGGGACATATTCAGGTTCCTGTAATTAAAGGATCTTATGAGAGTGCTTCTATCACAAGA GAGTCAGGAGACAGAGCTGGTCACAGTTGTGAGAAAGAAAACAGTTCAGAGGGCCCAACTCAGAAAAGTC TTCGTGAAGCCAGGGAAGAAGTTGGATATAAATCAAAGTTGAAGAATGAGAAAAAGGATTCAGAATTTCC TGTAGATATGCAACCAGATCCAAACGTGACTTCATGTACAGAAAACGTTTCTGCAGCAAAATTTGACTAC AAGCTAAACAATATTTTCAGACTTCATGAACTCCCAGTGAGCTGCATCGTCATGCATCCCTGCAGAGACT ATCTCATCTCCTGTAGTGAAGATCGTCTCTGGAAGATGGTGGGCCTCCCACAGGGCAATGTACTTCTTAC AGGATCTGGCCACACTGATTGGTTGTCTGGCTGCTGCTTCCATCCGAGTGGCAGCAAGTTGGCTACCTCA AGTGGTGACAGCACAATCAAACTATGGGACCTCAACAAAGGTGAATGCACCTTGACCCTTGAAGGACACA ACCATGCCGTATGGTCCTGCACCTGGCACTCCTGTGGTGACTTCGTGGCTTCTGCCTCACTGGATATGAC GAGTAAAATCTGGGATGTCAACAGTGAACGGTGCAGATACACTTTGTATGGACACACAGACTCTGTGAAC AGCATTGAGTTTTTCCCTTTCTCCAACATTCTTCTCACCGCTTCTGCAGACAAGACCTTGTCTGTGTGGG ATGCACGAACAGGTAAATGTGAGCAGTCTCTGTATGGTCACATGCATTCTGTCAATGATGCCACCTTCAC GCCTAGGGGTCACATAATAGCATCCTGTGATGCCCGTGGAGTTACAAAGCTGTGGGACTTCCGGAAACTC ATTCCAATCGTGTCCATTGATGTGGGTCCAAGTTCGGGAAATGAGGTGAACTTTGACCAGTCAGGTCGAG TTTTAGCTCAGGCGAGTGCCAACGGGATTATCCATTTGCTGGATCTTAAATCGGGGCAAATTCACAAACT GGTGGGCCACGAGAGTGAAGTGCACAGTGTGGTGTTTTCCCACCTGGGGGAAAATCTGTATTCTGGAGGC TCTGACGGCACCATTCGGTTGTGGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222592 representing NM_029160
Red=Cloning site Green=Tags(s) MAAPSGVPPLRVLEELGIGLSPTGEVTEAVTSEGAYYLEQVTITETSEDECEYEEIPDDNFSIPEGEEDL EKAIHIIGEQARDIHILEQQTILPARNVMQEAIEDFLCNFLIKMGMTRTLDCFQAEWYELIQKKGSDFKG LGNVPDVYSQVMLLETENKNLKKELKHFKQAAEKAKEDLLRTQKERDFHRMHHKRIVQEKNKLIADLKGL KLHYASYEPTIRVLHEKHHALLKEKMLTSLERDRAVGKISGLQATLKNIDMGHIQVPVIKGSYESASITR ESGDRAGHSCEKENSSEGPTQKSLREAREEVGYKSKLKNEKKDSEFPVDMQPDPNVTSCTENVSAAKFDY KLNNIFRLHELPVSCIVMHPCRDYLISCSEDRLWKMVGLPQGNVLLTGSGHTDWLSGCCFHPSGSKLATS SGDSTIKLWDLNKGECTLTLEGHNHAVWSCTWHSCGDFVASASLDMTSKIWDVNSERCRYTLYGHTDSVN SIEFFPFSNILLTASADKTLSVWDARTGKCEQSLYGHMHSVNDATFTPRGHIIASCDARGVTKLWDFRKL IPIVSIDVGPSSGNEVNFDQSGRVLAQASANGIIHLLDLKSGQIHKLVGHESEVHSVVFSHLGENLYSGG SDGTIRLWI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029160 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029160.3, NP_083436.2 |
| RefSeq Size | 2145 bp |
| RefSeq ORF | 1920 bp |
| Locus ID | 66722 |
| UniProt ID | Q8K450 |
| Gene Summary | Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219998 | Spag16 (untagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 1, (10ug) |
CNY 8,270.00 |
|
| MR222592 | Spag16 (Myc-DDK-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 1 |
CNY 4,760.00 |
|
| MR222592L3 | Lenti ORF clone of Spag16 (Myc-DDK-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 1 |
CNY 7,510.00 |
|
| MR222592L4 | Lenti ORF clone of Spag16 (mGFP-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 1 |
CNY 7,510.00 |
