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Siglec1 (NM_011426) Mouse Tagged ORF Clone
CAT＃: MG222509
- TrueORF®
Siglec1 (tGFP-tagged) - Mouse sialic acid binding Ig-like lectin 1 sialoadhesin (Siglec1), (10ug)
ORF Plasmid: DDK
"NM_011426" in other vectors (2)
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CNY 15,580.00
货期*
9周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cd169; Siglec-1; Sn |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222509 representing NM_011426
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGTCCTGTTCTCCCTGCTCCTGCTGGCCTCTGTCTTCTCACTAGGCCAGACCACATGGGGTGTCT CCAGTCCCAAGAATGTGCAGGGCTTGTCGGGATCCTGCCTGCTCATTCCCTGCATCTTCAGCTACCCTGC CGATGTCCCAGTGTCCAATGGCATCACAGCCATCTGGTACTATGACTACTCGGGCAAGCGGCAGGTGGTA ATCCACTCAGGGGACCCCAAGCTGGTGGACAAGCGTTTCAGGGGTCGAGCTGAACTGATGGGGAACATGG ACCACAAGGTGTGCAACCTGTTGCTCAAAGACTTGAAGCCTGAAGACTCTGGCACCTACAACTTCCGCTT TGAGATCAGTGATAGCAACCGCTGGTTAGATGTCAAAGGCACCACGGTCACTGTGACAACGGATCCCAGC CCCCCCACTATTACCATTCCTGAGGAGCTGCGTGAAGGCATGGAGAGGAACTTCAACTGTTCCACACCCT ACCTGTGCCTGCAGGAGAAGCAAGTCAGCCTGCAGTGGCGAGGCCAGGACCCCACCCACTCTGTCACCTC CAGCTTCCAGAGCCTCGAGCCCACTGGCGTCTATCACCAGACGACCCTACATATGGCCCTATCCTGGCAG GACCACGGTCGGACCCTGCTCTGCCAGTTCTCATTGGGCGCACACAGTAGTCGGAAAGAGGTTTACCTGC AAGTGCCACATGCCCCCAAAGGTGTGGAGATCCTCCTCAGCTCCTCAGGGAGGAACATCCTTCCCGGGGA TCCAGTCACACTCACCTGCAGAGTGAACAGCAGCTATCCTGCTGTCAGTGCCGTGCAGTGGGCCAGGGAC GGAGTGAACCTCGGAGTCACGGGACATGTGCTTCGGCTGTTCTCAGCAGCCTGGAATGATTCTGGGGCCT ACACCTGCCAAGCAACAAATGATATGGGCTCTCTGGTGTCATCCCCGCTCAGCCTCCATGTTTTTATGGC TGAAGTCAAAATGAACCCCGCAGGGCCCGTCTTGGAAAATGAGACAGTGACTCTGCTCTGTAGCACGCCG AAGGAGGCTCCCCAGGAGCTCCGCTATAGCTGGTACAAGAACCACATTCTCCTGGAAGATGCCCATGCCT CAACCTTGCACCTGCCTGCAGTCACCAGGGCTGATACTGGCTTCTACTTCTGTGAAGTGCAGAATGCCCA GGGCAGTGAGCGCTCCAGTCCATTGAGTGTGGTGGTCAGATATCCACCCCTTACTCCAGACCTGACCACC TTCCTGGAGACACAGGCCGGACTTGTGGGCATCTTGCATTGCTCCGTGGTCAGTGAGCCCCTGGCTACTG TGGTGCTGTCACACGGAGGCCTCACGTTGGCCTCCAACTCTGGAGAAAATGACTTCAACCCCCGATTCAG GATCTCCTCTGCCCCCAACTCCCTGCGCCTAGAAATCCGAGACTTGCAGCCAGCAGACAGCGGAGAGTAC ACATGCTTAGCTGTCAACTCCCTTGGAAACTCAACGTCCAGCCTAGACTTCTATGCTAATGTGGCCCGAC TCCTCATCAACCCTTCAGCAGAGGTTGTGGAAGGGCAGGCGGTGACCCTGAGCTGCAGGAGTGGCCTGAG CCCAGCTCCTGACACTCGCTTCTCCTGGTACCTGAACGGAGCTCTACTTCTGGAAGGATCCAGCAGCAGC CTCCTGCTTCCTGCGGCTTCCAGCACTGATGCGGGCTCATACTACTGTAGGACGCAGGCTGGCCCCAACA CCAGCGGCCCCTCCCTGCCTACTGTCCTCACTGTGTTCTATCCCCCAAGAAAGCCCACATTCACTGCCAG GCTGGATTTGGATACCTCTGGAGTCGGGGATGGACGACGGGGCATCCTCTTGTGCCACGTAGACAGCGAT CCCCCAGCCCAGCTACGGCTTCTCCACAAAGGCCATGTTGTGGCCACTTCTCTGCCATCAAGGTGTGGGA GCTGTTCCCAGCGCACAAAAGTCAGCAGAACCTCCAACTCACTGCACGTGGAGATCCAGAAGCCTGTATT AGAGGATGAGGGCGTGTACCTGTGTGAGGCTAGCAACACATTGGGCAACTCCTCAGCCGCAGCCTCTTTC AATGCTAAGGCCACTGTACTGGTCATCACACCGTCAAATACACTGCGTGAAGGCACAGAGGCCAACCTAA CTTGCAACGTGAACCAGGAGGTTGCTGTCAGCCCTGCCAACTTCTCCTGGTTCCGGAATGGAGTGCTGTG GACCCAGGGATCACTGGAGACTGTGAGGCTGCAGCCTGTGGCCAGAACTGATGCTGCTGTCTATGCCTGC CGCCTCCTCACCGAGGATGGGGCTCAGCTCTCGGCTCCTGTGGTCCTAAGTGTGCTGTATGCCCCAGACC CTCCAAAGCTGTCAGCCCTCCTAGATGTGGGTCAGGGCCACATGGCCGTGTTCATCTGCACTGTGGACAG CTATCCCCTGGCTCACCTGTCTCTGTTCCGTGGGGACCATCTCCTGGCCACCAACTTGGAACCCCAGCGT CCCTCCCATGGCAGGATCCAGGCCAAGGCCACAGCCAACTCCCTGCAGCTAGAGGTCCGAGAACTAGGTC TTGTGGACTCTGGAAACTACCACTGTGAAGCCACCAATATTCTTGGGTCAGCCAACAGTTCACTCTTCTT CCAGGTCAGAGGAGCCTGGGTCCAGGTTTCACCATCACCTGAGCTCCGGGAGGGCCAGGCTGTGGTCCTG AGCTGCCAGGTGCCCACAGGAGTCTCTGAGGGGACCTCATACAGCTGGTATCAGGATGGCCGCCCCCTCC AGGAGTCAACCTCATCTACACTCCGCATTGCAGCCATAAGTCTGAGGCAAGCTGGTGCCTACCATTGCCA AGCTCAGGCCCCAGACACAGCTATTGCCAGCCTGGCTGCCCCTGTCAGCCTCCATGTGTCCTATACCCCA CGTCATGTTACACTCAGTGCCCTGCTGAGCACGGACCCTGAGCGACTAGGCCACCTGGTGTGCAGTGTAC AAAGTGACCCTCCAGCGCAGCTGCAACTGTTTCACCGGAATCGCCTCGTGGCCTCTACCCTACAAGGCGC GGACGAATTGGCAGGCAGTAATCCCCGGCTGCATGTGACTGTGCTCCCCAATGAGCTGCGCCTGCAGATC CACTTTCCAGAGCTGGAGGATGACGGGACCTATACATGCGAAGCCAGCAACACACTGGGCCAGGCCTCGG CTGCAGCTGACTTCGATGCCCAGGCTGTGCGAGTGACTGTGTGGCCCAATGCCACTGTGCAAGAGGGGCA GCAGGTGAACCTGACCTGCTTGGTGTGGAGCACCCACCAGGACTCACTCAGCTACACATGGTACAAGGGC GGGCAACAACTCCTTGGTGCCAGATCCATCACCCTGCCCAGTGTTAAGGTTTTGGATGCTACCTCCTACC GCTGTGGTGTGGGGCTCCCCGGCCACGCACCCCATCTCTCCAGACCCGTGACCCTGGATGTCCTCCATGC TCCCCGAAACCTGCGGCTGACCTACCTCCTAGAGACCCAGGGCAGGCAGCTGGCCCTGGTACTGTGTACG GTGGATAGTCGTCCACCTGCCCAGCTAACTCTCAGCCATGGTGACCAGCTTGTAGCCTCCTCAACTGAAG CCTCTGTCCCCAACACCCTGCGCCTAGAGCTTCAGGATCCAAGGCCTAGTAATGAGGGGCTCTATAGCTG CTCTGCCCACAGCCCATTGGGCAAGGCCAACACGTCCCTGGAACTTCTGCTGGAAGGTGTCCGAGTGAAA ATGAATCCCTCTGGTAGTGTACCCGAGGGAGAGCCTGTCACAGTGACCTGCGAGGACCCTGCTGCCCTCT CATCCGCCCTCTATGCCTGGTTTCACAATGGCCATTGGCTTCAGGAGGGACCGGCTTCCTCACTCCAGTT CCTGGTGACTACACGGGCTCACGCTGGTGCTTACTTTTGCCAGGTGCATGATACACAAGGCACACGGAGC TCCAGACCTGCCAGCCTGCAAATTCTCTATGCCCCCCGGGATGCTGTCCTGTCTTCCTTTCGAGACTCAA GGACCAGGCTCATGGTCGTGATTCAGTGCACCGTGGACAGTGAGCCACCTGCTGAGATGGTCCTATCCCA CAATGGCAAGGTGCTAGCTGCCAGCCACGAGCGTCACAGCTCAGCATCAGGGATAGGCCACATCCAGGTA GCCCGAAATGCTCTTCGACTACAAGTGCAAGATGTGACTCTGGGTGATGGCAACACCTATGTTTGCACAG CCCAGAATACACTGGGCTCCATCAGTACCACCCAGAGGCTTCTGACGGAGACTGATATACGTGTGACAGC TGAGCCAGGCTTGGATGTGCCAGAGGGCACAGCTCTGAACTTAAGCTGCCTCCTCCCTGGTGGCTCTGGG CCCACGGGCAACTCTTCCTTCACGTGGTTCTGGAATCGCCACCGACTACATTCAGCTCCTGTGCCCACAC TCTCCTTCACCCCTGTGGTCCGGGCTCAGGCTGGGCTGTACCACTGCAGGGCTGATCTCCCCACCGGGGC CACTACCTCTGCTCCAGTTATGCTCCGTGTCCTCTATCCCCCCAAGACGCCCACTCTCATAGTGTTTGTG GAGCCTCAGGGTGGCCACCAGGGCATCCTCGACTGTCGAGTGGACAGTGAGCCCCTGGCCATCCTCACTC TTCACCGGGGCAGTCAACTAGTAGCCTCCAACCAACTTCACGATGCTCCCACCAAGCCCCACATCCGAGT CACTGCTCCTCCCAATGCCTTGAGAGTGGACATAGAGGAGCTCGGCCCTAGCAATCAAGGGGAGTATGTG TGCACTGCCTCCAACACTCTGGGCTCTGCCTCAGCCTCTGCCTACTTTGGGACCAGAGCTCTGCACCAAC TGCAGCTGTTCCAGAGGCTGCTCTGGGTCCTGGGATTTCTGGCAGGCTTCCTGTGCCTGCTGCTGGGTCT GGTGGCCTATCACACCTGGAGAAAGAAGAGTTCTACCAAGCTGAATGAGGATGAGAATTCAGCAGAGATG GCCACTAAGAAAAATACTATCCAGGAGGAAGTGGTTGCTGCTCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222509 representing NM_011426
Red=Cloning site Green=Tags(s) MCVLFSLLLLASVFSLGQTTWGVSSPKNVQGLSGSCLLIPCIFSYPADVPVSNGITAIWYYDYSGKRQVV IHSGDPKLVDKRFRGRAELMGNMDHKVCNLLLKDLKPEDSGTYNFRFEISDSNRWLDVKGTTVTVTTDPS PPTITIPEELREGMERNFNCSTPYLCLQEKQVSLQWRGQDPTHSVTSSFQSLEPTGVYHQTTLHMALSWQ DHGRTLLCQFSLGAHSSRKEVYLQVPHAPKGVEILLSSSGRNILPGDPVTLTCRVNSSYPAVSAVQWARD GVNLGVTGHVLRLFSAAWNDSGAYTCQATNDMGSLVSSPLSLHVFMAEVKMNPAGPVLENETVTLLCSTP KEAPQELRYSWYKNHILLEDAHASTLHLPAVTRADTGFYFCEVQNAQGSERSSPLSVVVRYPPLTPDLTT FLETQAGLVGILHCSVVSEPLATVVLSHGGLTLASNSGENDFNPRFRISSAPNSLRLEIRDLQPADSGEY TCLAVNSLGNSTSSLDFYANVARLLINPSAEVVEGQAVTLSCRSGLSPAPDTRFSWYLNGALLLEGSSSS LLLPAASSTDAGSYYCRTQAGPNTSGPSLPTVLTVFYPPRKPTFTARLDLDTSGVGDGRRGILLCHVDSD PPAQLRLLHKGHVVATSLPSRCGSCSQRTKVSRTSNSLHVEIQKPVLEDEGVYLCEASNTLGNSSAAASF NAKATVLVITPSNTLREGTEANLTCNVNQEVAVSPANFSWFRNGVLWTQGSLETVRLQPVARTDAAVYAC RLLTEDGAQLSAPVVLSVLYAPDPPKLSALLDVGQGHMAVFICTVDSYPLAHLSLFRGDHLLATNLEPQR PSHGRIQAKATANSLQLEVRELGLVDSGNYHCEATNILGSANSSLFFQVRGAWVQVSPSPELREGQAVVL SCQVPTGVSEGTSYSWYQDGRPLQESTSSTLRIAAISLRQAGAYHCQAQAPDTAIASLAAPVSLHVSYTP RHVTLSALLSTDPERLGHLVCSVQSDPPAQLQLFHRNRLVASTLQGADELAGSNPRLHVTVLPNELRLQI HFPELEDDGTYTCEASNTLGQASAAADFDAQAVRVTVWPNATVQEGQQVNLTCLVWSTHQDSLSYTWYKG GQQLLGARSITLPSVKVLDATSYRCGVGLPGHAPHLSRPVTLDVLHAPRNLRLTYLLETQGRQLALVLCT VDSRPPAQLTLSHGDQLVASSTEASVPNTLRLELQDPRPSNEGLYSCSAHSPLGKANTSLELLLEGVRVK MNPSGSVPEGEPVTVTCEDPAALSSALYAWFHNGHWLQEGPASSLQFLVTTRAHAGAYFCQVHDTQGTRS SRPASLQILYAPRDAVLSSFRDSRTRLMVVIQCTVDSEPPAEMVLSHNGKVLAASHERHSSASGIGHIQV ARNALRLQVQDVTLGDGNTYVCTAQNTLGSISTTQRLLTETDIRVTAEPGLDVPEGTALNLSCLLPGGSG PTGNSSFTWFWNRHRLHSAPVPTLSFTPVVRAQAGLYHCRADLPTGATTSAPVMLRVLYPPKTPTLIVFV EPQGGHQGILDCRVDSEPLAILTLHRGSQLVASNQLHDAPTKPHIRVTAPPNALRVDIEELGPSNQGEYV CTASNTLGSASASAYFGTRALHQLQLFQRLLWVLGFLAGFLCLLLGLVAYHTWRKKSSTKLNEDENSAEM ATKKNTIQEEVVAAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011426 |
| ORF Size | 5103 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011426.2 |
| RefSeq Size | 6427 bp |
| RefSeq ORF | 5106 bp |
| Locus ID | 20612 |
| UniProt ID | Q62230 |
| Gene Summary | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hematopoiesis. May act as a counter-receptor for CLEC10A in lymph node.[UniProtKB/Swiss-Prot Function] |
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