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Prdm8 (NM_029947) Mouse Tagged ORF Clone
CAT＃: MG222292
- TrueORF®
Prdm8 (tGFP-tagged) - Mouse PR domain containing 8 (Prdm8), (10ug)
ORF Plasmid: DDK
"NM_029947" in other vectors (2)
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CNY 6,370.00
货期*
4周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930565F05Rik; PFM5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222292 representing NM_029947
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATTCAGGCATCCAGAGAGGCATCTGGGATGGAGATGCCAAGGCTGTCCAACAGTGTCTGACAG ACATTTTTACCAGTGTATATACAACCTGCGACATCCCAGAGAACGCCATATTCGGTCCCTGTGTACTGAG CCATACTTCCCTGTATGACAGCATAGCCTTCGTAGCCCTCAAGTCCACGGACAAGAGAACAGTACCTTAC ATCTTCCGGGTAGACACTTCGGCGGCAAATGGTTCCTCAGAAGGTCTCATGTGGCTGCGGCTGGTCCAAT CAGCCCGAGATAAGGAAGAACAGAATCTCGAAGCTTATATAAAAAATGGACAGCTGTTCTACCGCTCTCT CCGCAGGATTGCCAAAGATGAAGAGTTACTAGTTTGGTACGGGAAAGAACTGACTGAATTGCTCTTGCTC TGCCCGTCTAGAGCTCACAAAATGAACGGGTCGTCTCCTTACACATGCCTGGAATGCAGCCAACGTTTCC AGTTTGAGTTCCCCTATGTGGCACATCTGCGATTCCGCTGCCCCAAGAGACTTCACAGCACTGATGCGAA TCCCCAAGACGAGCAAGGGGGCGGCTTGGGCACTAAGGACCACGGCGGCGGCGGCGGTGGTAAAGAGCAA CAGCAGCAGCAACAGCAGCAGCAACAGGAGGCGCCGTTGATCCCGGGCCCCAAGTTCTGCAAAGCCGGCC CCATACACCACTACCCCGCGTCATCCCCGGAGGCGAGCAACCCGCCGGGCTCCGCGGGTGCCGGCAGCGC CAAGCCGTCCACGGACTTCCACAACTTGGCTCGGGAACTGGAAAACTCCCGGGGAAGCAGCAGTTGCGTA GCGGCCCCGGGCGTCGGCAGTGGCGGCAGCGGCCACCAGGAGGCGGAGCTGAGTCCCGACGGCGTCGCCA CCGGCGGCTGCAAAGGCAAGAGGCGGTTCCCGGAGGAGGCGGCGGCGGAGGGCGGCGGCGCGGGGCTGGC GGGCGGCCGTGCGCGCTTCTCCGAGAGGCCGCTGGCGACCTCCAAGGAGGAGTTGGTGTGCACGCCGCAG CAGTACCGCGCCGCCGGCAGCTACTTCGGCCTGGAGGAGAACGGCCGGCTCTTCGCGCCGCCCAGCCCGG AGACTGGCGAGGCGAAGCGTAGCGCCTTCGTGGAGGTGAAGAAGGCGGGCCGCGCGGTGGGCTTGCAGGA GGAGGCGGCGGCGACAGACGGCGCAGGGGGCACGGCCGAGGACCCCGACGCGGGAGGCGGTGTCGCCGGC GGCGGCAGCAACGGCTCGTCCACCCCCGCGGCGGGGTCGCCTGGGGCCCCCGAGAAGTTGCTGGCTCCGC GTCCCGGAGGCTCTCTGCCCGGACGGCTGGAGGGCGGGAGCCCCGCGCGCGGCAGCGCCTTCACCTCGGT GTCGCAGCTGGGCGGCGGTGGCGGCGCGGGGACCGCGGGGACTGCGGGGGGCTCGGGGGGCGGCCAAACG GCCGCATCGGACGAGCGGAAGAGCGCCTTCTCGCAGCCCGCGCGCTCCTTCTCGCAGCTGTCCCCGTTGG TCCTCGGCCAGAAGCTGGGCGCTCTCGAGCCGTGTCACCCGGGAGACGGCGTGGGTCCCACCAGACTCTA CCCGGCCGCCGCTGATCCGCTGGCTGTGAAGCTGCAGGGGGCAGCGGACCTGAACGGAGCCTGCGGGCCC CTGGCGAGCGGCGGTGGCGGTGGCTTGCCCAAGCAGAGCCCCTTCCTCTACGCCACCGCCTTCTGGCCCA AGAGCTCGGCTGCGGCGGCAGCGGCGGCGGCGGCCGCCGCCGGGCCCCTGCAGCTGCAACTGCCCTCCGC GCTCACCCTGCTGCCGCCCTCCTTCACTTCGCTGTGTCTGCCCGCGCAGAACTGGTGCGCCAAATGCAAC GCCTCCTTCCGCATGACCTCCGACCTGGTGTACCATATGCGGTCTCACCACAAAAAGGAGTATGCCATGG AGCCCCTGGTGAAGCGGCGGCGGGAGGAGAAACTCAAGTGCCCCATTTGCAACGAGTCCTTCAGGGAGCG TCACCACCTGTCCCGGCACATGACCTCGCATAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222292 representing NM_029947
Red=Cloning site Green=Tags(s) MEDSGIQRGIWDGDAKAVQQCLTDIFTSVYTTCDIPENAIFGPCVLSHTSLYDSIAFVALKSTDKRTVPY IFRVDTSAANGSSEGLMWLRLVQSARDKEEQNLEAYIKNGQLFYRSLRRIAKDEELLVWYGKELTELLLL CPSRAHKMNGSSPYTCLECSQRFQFEFPYVAHLRFRCPKRLHSTDANPQDEQGGGLGTKDHGGGGGGKEQ QQQQQQQQQEAPLIPGPKFCKAGPIHHYPASSPEASNPPGSAGAGSAKPSTDFHNLARELENSRGSSSCV AAPGVGSGGSGHQEAELSPDGVATGGCKGKRRFPEEAAAEGGGAGLAGGRARFSERPLATSKEELVCTPQ QYRAAGSYFGLEENGRLFAPPSPETGEAKRSAFVEVKKAGRAVGLQEEAAATDGAGGTAEDPDAGGGVAG GGSNGSSTPAAGSPGAPEKLLAPRPGGSLPGRLEGGSPARGSAFTSVSQLGGGGGAGTAGTAGGSGGGQT AASDERKSAFSQPARSFSQLSPLVLGQKLGALEPCHPGDGVGPTRLYPAAADPLAVKLQGAADLNGACGP LASGGGGGLPKQSPFLYATAFWPKSSAAAAAAAAAAAGPLQLQLPSALTLLPPSFTSLCLPAQNWCAKCN ASFRMTSDLVYHMRSHHKKEYAMEPLVKRRREEKLKCPICNESFRERHHLSRHMTSHN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029947 |
| ORF Size | 2064 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029947.2, NP_084223.2 |
| RefSeq Size | 3119 bp |
| RefSeq ORF | 2067 bp |
| Locus ID | 77630 |
| UniProt ID | Q8BZ97 |
| Gene Summary | Probable histone methyltransferase, preferentially acting on 'Lys-9' of histone H3 (PubMed:19646955). Histone methyltransferase activity has not been confirmed in other species. Involved in the control of steroidogenesis through transcriptional repression of steroidogenesis marker genes such as CYP17A1 and LHCGR (PubMed:19646955). Forms with BHLHE22 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation (PubMed:22284184). In the retina, it is required for rod bipolar and type 2 OFF-cone bipolar cell survival (PubMed:26023183).[UniProtKB/Swiss-Prot Function] |
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